Mass Spectrometry
Sample Preparation
For low resolution mass spectrometry (LRMS)
1.
2.
3.
4.
5.
0.5-1 mg/mL in water or in organic solvents preferably acetonitrile or methanol.
DCM or CHCl3 or EtOAc are less favourable.
Use 2 mL HPLC glass vials (dimension 12x32 mm (diameter x height)).
Make the final volume 1 mL approximately.
Always have your expected molecular weight ready and the chemical formula for filling
up the log-sheet prior to sample analysis.
For high resolution mass spectrometry (HRMS)
1. Sample must be pure.
2. Make 0.1-0.5 mg/mL of the pure sample in water or organic solvents mentioned in
LRMS section.
3. Provide sample as solid (~1 mg) or oil (~1 mL) if the sample degrades over time. In such
case, mention the appropriate solvent that the sample dissolves in.
4. ChemDraw structure along with Exact Mass to 4 decimal points must be provided.
5. Please mention whether sample is hazardous or requires any special handling.
Sample preparation for mass spectrometry
NOTE: Samples cannot contain the following:
1. SALT BUFFERS (e.g. phosphate, citrate, borate, etc.)
2. DETERGENTS
3. INORGANIC ACIDS (e.g. H2SO4, H2PO4)