NUS Institutional Animal Care and Use Committee
000/15
Date revised: Jun 2015
Form expiry date: 31 Dec 2015
Appendix 6
Changes in animal breeding protocol
Please check the appropriate box(es) and complete only the relevant Section(s).
1. Deleting existing colony species/strains
2. Adding new colony species/strains
3. Change in personnel
4. Change in colony housing location
5. Other changes
Complete the relevant section(s) applicable
1. Deleting existing colony species/strains
1.1
Please indicate the species/strains to be deleted:
1.2
Please explain why the change is required:
1.3
Disposition of the animal species/strains to be deleted:
N.A. Breeding of the species/strains to be deleted has not started.
Animals will be euthanized.
IACUC encourages sharing of tissues to minimise animal use. If you agree to provide
tissues from the euthanized animals, please submit the Tissue Sharing form.
Tissue sharing form attached.
Tissue sharing form not attached.
Others. Please describe their final disposition:
1.4
For each species/strains to be deleted, provide the number of desired and
undesired animals (phenotype) produced, the number used for experiment,
and the number of breeders used to obtained the species/strains since the last
annual protocol review:
N.A. Breeding of the species/strains to be deleted has not started.
Species/strains
No. of
breeders
used
Undesired produced
No. held
No.
and to be
euthanized
disposed
Desired produced
No. held
No. used /
and to be
euthanized
disposed
1.
2.
3.
4.
5.
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2. Adding new colony species /strains
2.1
Please explain why breeding of the new species/ strains is required: (Please
note that breeding is not allowed for species/strains that are commercially available
from local suppliers)
2.2
Information on animals to be bred and protocol served.
(a) Please complete the table below. If you are breeding genetically modified
animals, please indicate the exact modification and the rationale for its
use*. This rationale must include type of modification (e.g., KO, Tg, KI,
naturally occurring mutant), the construct and function of the construct or
gene to be modified).
Please provide information for each strain and or construct (submit a separate sheet if
necessary):
(1)
Species
Background Strain
Construct*
Modification*
Function of the
gene modified* or
the consequence of
the modification
IACUC protocol
number served1
Tg
KO
KI
Other:
My own research protocol (Protocol no.
)
Number of animals to be transferred to this protocol:
Other NUS PI ‘s protocol (Protocol No:
)
Number of animals to be transferred to this protocol:
Other institution PI’s protocol (Protocol No:
)
Name of PI and institution :
Number of animals to be transferred to this protocol:
(2)
Species
Background Strain
Construct*
Modification*
Function of the
gene modified* or
the consequence of
the modification
IACUC protocol
number served1
Tg
KO
KI
Other:
My own research protocol (Protocol no.
)
Number of animals to be transferred to this protocol:
Other NUS PI ‘s protocol (Protocol No:
)
Number of animals to be transferred to this protocol:
Other institution PI’s protocol (Protocol No:
)
Name of PI and institution :
Number of animals to be transferred to this protocol:
(3)
Amendment
Species
Background Strain
2
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NUS Institutional Animal Care and Use Committee
Construct*
Modification*
Function of the
gene modified* or
the consequence of
the modification
IACUC protocol
number served1
Tg
KO
000/15
KI
Other:
My own research protocol (Protocol no.
)
Number of animals to be transferred to this protocol:
Other NUS PI ‘s protocol (Protocol No:
)
Number of animals to be transferred to this protocol:
Other institution PI’s protocol (Protocol No:
)
Name of PI and institution :
Number of animals to be transferred to this protocol:
1
IACUC protocol served is the research protocol to which animals will be transferred for
experiment. No experimental procedure described in the research protocol served should be
conducted before the transfer.
(b) Principal Investigators are responsible for ensuring that the number of animals
bred should not grossly exceed the estimated number required by the protocol
served and to undertake the following:
An up to date record of the number of animals bred, used, euthanized and transferred has
to be maintained and produced on request. Please refer the Annual Protocol Review Form
for Breeding Colony for the information required from the record.
The number of animals transferred to a research protocol served must not exceed the
number approved for the research protocol. (If more animals are required for research
protocol, an amendment of the research protocol must be approved by IACUC before the
transfer).
A notification must be sent to IACUC when transferring animals to another NUS PI (email
dprnmya@nus.edu.sg specifying the species/strains, animal number and protocol number
served).
Comply with CM requirements for transfer of animals e.g., submission of the ‘Internal
Animal Transfer Record’ form to CM. Animal shipping out of CM must pass through CM.
All animals whether desired or undesired produced must be used in accordance with an
approved protocol. (e.g., undesired animals produced should not be used for practice
without IACUC approval).
2.3 Source, number and housing location of breeders.
(a) Please indicate the source of animal for initiating the breeding programme.
Please note that CM and/or IACUC have to be informed prior to any movement of animals
into or out of NUS, including research laboratories (e.g., importation, transfer from another
institution, delivery by local suppliers to the labs, etc).
Sources obtained through CM. Species/strains:
My approved breeding protocol No:
Species/stains:
Other PI’s approved breeding protocol No:
Species/stains:
Other sources obtained directly by PI. (Only for species caught from the wild by
researches. All laboratory animals including fish and frog should be obtained
through CM)
Please indicate the species/strains and source, including the names and addresses
of the suppliers, if applicable:
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(b) Please indicate the number of new breeders required for initiating the breeding
programme and for future replacement / backcross.
Note: Supply of animals by CM will be based on the information provided in Table below.
Inadequate or inaccurate information will result in delays in the animal acquisition
process. All animal imports must be made through CM for quarantine (where applicable)
and monitoring of animal use (an AVA requirement).
N.A. This is a protocol renewal. Breeding colonies already established in CM under
protocol No:
Name of species/ strains
No. required
for initiating
the breeding
programme
No. require from
source for
replacement /
backcross
No.
1
2
3
♀
♂
♀
♂
♀
♂
breeder:
breeder:
breeder:
breeder:
breeder:
breeder:
♀:
♂:
♀:
♂:
♀:
♂:
♀:
♂:
♀:
♂:
♀:
♂:
Animal housing
location
Frequency
(c) Indicate the breeding scheme to be used:
Note: Please read IACUC Policy on Mouse Cage Density on breeding schemes.
Paired breeding
IACUC approved trio breeding
Alternative to IACUC approved trio breeding. (Please indicate species/strains and
provide justification):
Others. (Please describe and justify):
(d) Age of breeders
(i) Age at which the breeders will be used for breeding:
(ii) Age at which the breeders will be replaced:
2.4
Estimation of number of offspring to be produced under this breeding
protocol:
The IACUC understands that you will not be able to provide accurate numbers. However, you
should be able to give an estimate of the number of animals you intend to breed in order to
obtain the desired offspring for your research protocol.
Note: You may not be able to provide an estimate of the number supply to other PIs. An
amendment should be submitted when the total number will be exceeded due to the
unexpected number you supply.
(a) Please provide an estimate in the table below:
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Estimated number of offspring per year
Name of offspring
(Strains/construct)
No. to be
used as
replacement
breeders
No. of
desired
(Animals that
will be
transferred to
protocol
served for
experimental
manipulations)
No. of
undesired
(Animals that
cannot be used
in experiments
e.g wrong
genotype or sex)
Total
Note: You are required to keep accurate records of the number of animals produced, culled
or transferred to research protocols. This information will be requested annually.
(b) Explain how the numbers of desired and undesired offspring are estimated.
2.5
Disposition of undesired animals produced:
Undesired animals will be euthanized.
(Note: undesired animals should not be used for practice, training or any other purposes
without the approval of IACUC)
IACUC encourages sharing of tissues to minimise animal use. If you agree to provide
tissues from the euthanized animals to other researcher, please submit the Tissue
Sharing form.
Tissue sharing form attached.
Tissue sharing form not attached.
Others. Please describe their final disposition:
2.6
Method of animal identification:
Note: Toe clipping is not acceptable as a method of animal identification.
Ear notch
Ear punch
Others (Please describe):
2.7
Ear tag
Tattoo
Cage card only
Method of genotyping:
Tail snipping according to CM SOP for tail snipping #401.02.
Blood sampling (Attach Procedure B
)
Ear punching
Others (Please describe):
2.8
Procedure to be conducted under the breeding protocol:
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Note: Only for procedure related to breeder manipulation. Animals should not be subjected to
experimental procedure described in the research protocol served. Please provide
details of procedure in Procedure A - F form)
N.A.
Administration of drugs, e.g., ovulation agent. (Attach Procedure B
)
Surgical procedure, e.g., vasectomy and caesarean section. (Attach Procedure A
)
Behavioural tests, e.g., for selection of suitable experimental model (Attach Procedure B
Blood/tissue/fluid collection. (Attach Procedure B
)
Others (Please describe and attach relevant Procedure form
):
2.9
)
Will the animals require any special handling or care (e.g., weaning before or
after the recommended 21 days, acidified water, special diet, etc.)
Please read the IACUC Policy on Mouse Cage Density on prevention of overcrowding
of animals.
No.
Yes. Please identify the affected strains and describe in detail and discuss these
expectations with the facility manager:
2.10 Measures to lessen impact of genetic drift while maintaining the breeding
colony.
Please read the information on genetic drift from the Jackson Laboratory.
Please indicate the measure(s) you will take:
The colony maintained by non-sib matings is not expanded beyond three generations.
The colony maintained by sib matings is not expanded beyond ten generations.
Backcross with animals from the source every
generations.
Refresh the breeders from the source every
generations.
Others. Please describe:
2.11 Are there any known or suspected pathologic susceptibilities inherent to the
animal or strain that may cause pain and suffering? This includes natural
susceptibilities resulting from genetic modification, such as carrying a
transgene: (Please attach the strain data sheet from the supplier)
No.
Yes. Please provide:
(a) Description of the pathologic susceptibilities of each affected strains:
(b) Special husbandry procedures for these animals:
(c) Provide the criteria for early euthanasia (premature termination due to the
pathologies) in 2.12 below:
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2.12 Do the animals have a known potential to generate a pathogen or hazardous
material/compound that could be transmitted to humans or other animals?
No
Yes. Please identify the affected strains and describe the containment requirements and
any necessary precautions for animal caretakers in detail:
2.13 Criteria for early euthanasia of animals (breeders and offspring before transferring to
research protocol) if they are experiencing unrelieved pain or distress.
Criteria
e.g. Body weight loss
→
Specific clinical signs
How assessed
20% body weight loss compared to
control. The veterinarian will be informed
when the weight loss has reached 10% →
Frequent weighing of 3
times a week (on alternate
days)
1
2
3
4
5
2.14 Method of euthanasia
(a). Method of euthanasia:
Species
Agent
Dose
Volume
Route of admin.
(b). Method of ensuring that animals are dead:
Bilateral thoracotomy of the euthanized animal
Cervical dislocation of the euthanized animal
Others:
2.15 What is the estimated steady state breeding colony size at any one time?
Please provide the estimated number of cages required
Number of cages:
2.16 IACUC office maintains a list of genetically modified mice available in NUS for
NUS researchers. Would you like to be included in the list?
No.
Yes.
3. Change in personnel
3.1 Adding personnel
Please provide the following information for the new person who will be
primarily responsible for the care and maintenance of the breeding colony.
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Please note: Prior to submitting an amendment to add personnel, please ensure these
individuals have completed the RCULA training course and have a medical history
questionnaire on file with the infirmary, under the OSHE Occupational Health programme for
Personnel with Laboratory Animal Contact.
1. Full Name:
Status:
Faculty
Staff
Graduate student
Others:
Role:
PI
Co-PI
Visiting Scientist
Others:
Email:
NUS ID:
Phone:
Department:
RCULA Cert No;
OH Programme serial No:
Which species will this person handle in this
protocol?
Will this person perform any procedure listed in Section 2.7 above?
No
Yes Please list the procedures:
Please describe the person’s qualifications, training and experience with breeding
techniques and colony maintenance:
Is this person involved in any animal work outside NUS or working on animals from
outside NUS?
Yes. Please provide name of institution:
No. Please note that IACUC office must be inform if any of the personnel will be
involved in animal work outside NUS or working on animals from outside NUS.
2. Full Name:
Status:
Faculty
Staff
Graduate student
Others:
Role:
PI
Co-PI
Visiting Scientist
Others:
Email:
NUS ID:
Phone:
Department:
RCULA Cert No;
OH Programme serial No:
Which species will this person handle in this
protocol?
Will this person handle animal tissue in this protocol?
No
Yes
Will this person perform any procedure listed in Section 2.7 above?
No
Yes Please list the procedures:
Please describe the person’s qualifications, training and experience with breeding
techniques and colony maintenance:
Is this person involved in any animal work outside NUS or working on animals from
outside NUS?
Yes. Please provide name of institution:
No. Please note that IACUC office must be inform if any of the personnel will be
involved in animal work outside NUS or working on animals from outside NUS.
3.2 Removing personnel
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List the personnel to be removed and attach the CM Revocation form, a copy of which should
also be faxed to CM Office.
Full Name
Staff /student ID
3.3 Change in PI or person responsible for the protocol.
Please submit Appendix 4
4. Change in colony housing location
4.1
Please specify the location:
(Note: Please note that amendment is NOT required for change in housing location within CM
facilities without change in ABSL and approved by CM)
New location
Approved location
Building:
Building:
New location is additional location.
New location is replacing the approved location.
4.2
Reason(s) for the change:
5. Other changes
5.1
Please describe the change(s) in details:
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Appendix 6