UNIVERSITY OF MINNESOTA
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)
Appendix B
Protocol Specific Breeding
Note: If you intend to breed animals for use by multiple researchers or multiple protocols, complete
the Breeding Protocol Application Form for the use of the breeding, as opposed to this appendix.
You are required to keep accurate records of the number of animals produced and their ultimate
disposition. This information may be requested annually.
For more information on the costs and management of rodent breeding colonies see the RAR website.
1. Please explain the need for breeding on this protocol.
2. List the species and strains of animals to be produced in this breeding program:
3. Anticipated total number of offspring for each species that will be produced:
Animal Numbers
Species:
Breeders:
Species:
Breeders:
Species:
Breeders:
Total for 3 years
Offspring:
Offspring:
Offspring:
(Suggestion: Be sure this number matches the numbers provided in the Animal Request Table in Section 1 of the
Animal Care and Use Protocol form.)
4. Anticipated number of offspring that will be used for experiments:
5. What will be done with the surplus animals?
(Note: Certain animals may be donated to the Raptor Rehabilitation Center in lieu of euthanasia. These
animals are euthanized and used as food for the birds of prey in the rehabilitation program at the Gabbert
Raptor Center on the St. Paul Campus. See the RAR Web site for further details:
Breeding Genetically Modified Animals
Appendix B
Rev 3/11
UNIVERSITY OF MINNESOTA
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)
Note: If crossing pre-existing genetically modified strains, please contact the IBC (612-626-5654;
ibc@umn.edu) to determine if IBC approval is required.
6. DNA/transgene or gene to be disrupted:
7. Are there any inherent problems for the animal associated with the genetically modified
phenotype?
No.
Yes.
If yes, describe:
8. Method of monitoring presence of transgene in the animals. (Please note that if tail snipping is used it
must be performed according to IACUC guidelines .
9. Describe any special care or monitoring that may be required for these animals:
10. Describe who will perform this monitoring?
11. How long are the founders maintained?
12. What are the criteria for euthanasia of the founders?
Appendix B
Rev 3/11