曹鸿志
博士 副教授
一、个人情况简介：
出生年月：1974-10
工作单位：山东大学国家糖工程技术研究中心
通讯地址：济南市文化西路 44 号，
山东大学西校 87＃信箱
邮编:250012
E-mail: hzcao@sdu.edu.cn;
电话：0531-88382235(O); 15615515618
二、教育经历：
2000.9-2004.7
中国科学院上海有机化学研究所生命有机国家重点实验室，
理学博士，导师为俞飚研究员；
1997.9-2000.7
郑州大学化学系，理学硕士，导师为刘宏民教授；
1993.9-1997.7
郑州大学化学系，理学学士，论文指导老师为郭彦春教授。
三、工作经历：
2010.1－至今：
山东大学国家糖工程技术研究中心，副教授；
2005.12－2009.12 加州大学戴维斯分校化学系，博士后；
2005.6－2005.12：中美合资上海美迪西生物医药有限公司化学部，课题组长；
2004.7―2005.6： 中国科学院上海有机化学研究所生命有机国家重点实验室，
科研助理。
四、研究方向：
糖化学、糖化学生物学、复杂寡糖的化学生物合成、糖药物
五、承担的科研项目：
1.国家自然科学基金面上项目（21372145），2014.01-2017.12,负责人
2.国家自然科学基金面上项目（21172135），2012.01-2015.12,负责人
3.国家自然科学基金青年项目（20902087），2010.01-2012.12,负责人
4.山东省自然基金面上项目(ZR2010BM018),2010.11-2013.11，负责人
5.生命有机化学国家重点实验室开放课题，2011.11-2013.10，负责人
6.国家重点基础研究发展计划（973）(2012CB822100),2012.1-2016.12，
1
科研骨干
7.国家高技术研究发展计划（863）（2012AA021500），2012.1-2015.12，
科研骨干。
六、 发表论文：(*为通讯作者)
A 独立工作后发表论文：
（山东大学，2010 至今）
1. Zhang, Y.; Meng, C.; Chen, X.; *Wang, F.; *Cao, H. Chemoenzymatic synthesis of
-dystroglycan core M1 O-mannose glycans. submitted
2. Chen, C.; Zhang, Y.; Xue, M.; Liu, X.; Li, Y.; *Chen, X.; *Wang, P. G.; *Wang, F.;
*Cao, H. Sequential one-pot multienzyme (OPME) synthesis of
lacto-N-neotetraose and its sialyl and fucosyl derivatives. Chem. Comm. 2015, 51,
7689-7692.
3. Zhang, Y.; Chen, C.; Jin, L.; Tan, H.; *Wang, F.; *Cao, H. Synthesis of
unsymmetrical 3,6-branched Man5 oligosaccharide: a comparison between
one-pot sequential glycosylation and stepwise synthesis. Carbohydr. Res. 2015,
401, 109-114.
4. Yao, W.; Yan, J.; Chen, X.; Wang, F.; *Cao, H. Chemoenzymatic synthesis of
lacto-N-tetrasaccharide and sialyl lacto-N-tetrasaccharides. Carbohydr. Res.,
2015, 401, 5-10.
5. Sun, Y.; Sun, W.; Guo, J.; Hu, X.; Gong, G.; Huang, L.; Cao, H.; *Wang, Z.
Sulphation pattern analysis of chemically sulphated polysaccharide LbGp1 from
Lycium barbarum by GC-MS. Food Chem. 2015, 170, 22-9.
6. Lian, Q.; Cao, H.; *Wang, F. The cost-efficiency realization in the Escherichia
coli-based cell-free protein synthesis systems. Appl. Biochem. Biotechnol. 2014,
DOI: 10.1007/s12010-014-1143-4.
7. Meng, X.; Yao, W.; Cheng, J.; Zhang, X.; Jin, L.; Yu, H.; Chen, X.; *Wang, F.; *Cao,
H. Regioselective chemoenzymatic synthesis of ganglioside disialy
tetrasaccharide epitopes. J. Am. Chem. Soc. 2014, 136(14), 5205-5208.
8. Yan, J.; Chen, X.; *Wang, F.; *Cao, H. Chemoenzymatic synthesis of mono- and
di-fluorinated Thomsen-Friedenreich (T) antigens and their sialylated derivatives.
Org. Biomol. Chem. 2013, 11(5), 842-848.
9. Huynh, N.; Aye, A.; Li, Y.; Yu, H.; Cao, H.; Tiwari, V.; Shin, D.; Chen, X.; *Fisher, A.
Structureal
basis
for
substrate
specificity
and
mechanism
of
N-acetyl-D-neuraminic acid lyase from Pasteurella multocida. Biochemistry 2013,
52(47), 8570-8579.
10. Kikkeri, R.; Padler-Karavani, V.; Diaz, S.; Verhagen, A.; Yu, H.; Cao, H.; Langereis,
M.; De Groot, R.; Chen, X.; *Varki, A. Quantum dot nanometal surface energy
transfer based biosensing of sialic acid compositions and linkages in biological
samples. Anal. Chem. 2013, 85(8), 3864-3870.
11. Zong, A.; Zhao, T.; Zhang, Y.; Song, X.; Shi, Y.; Cao, H.; Liu, C.; Cheng, Y.; Qu, X.;
Cao, J.; *Wang, F. Anti-metastatic and anti-angiogenic activities of sulfated
polysaccharide of Sepiella maindroni ink. Carbohydr. Polym. 2013, 91(1),
403-409.
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12. Yin, X.; Yan, J.; *Ji, S.; *Wang, F.; *Cao, H. Recent advances in the synthesis of
heparin sulfate oligosaccharides. Chin. J. Org. Chem., 2012, 32(8), 1388-1400.
13. Zong, A.; Cao, H.; *Wang, F. Anticancer polysaccharides from natural resources:
a review of recent research. Carbohydr. Polym. 2012, 90(4), 1395-1410.
14. *Cao, H.; *Chen, X. General consideration on sialic acid chemistry. Methods
Mol. Biol., 2012, 808, 31-56.
15. *Cao, H.; Hwang, J.; *Chen, X. Carbohydrate-containing natural products in
medicinal chemistry. In Opportunity, Challenge and Scope of Natural
Products in Medicinal Chemistry. 2011, 411-431.
B．研究生及博士后期间发表论文：
16. Li, Y.; Yu, H.; Cao, H.; Muthana, S.; *Chen, X. Pasteurella multocida CMP-sialic acid
synthetase and mutants of Neisseria meningitidis CMP-sialic acid synthetase with
imporved substrate promiscuity. Appl. Microbiol. Biotechnol. 2012, 93(6), 2411-2423.
PMID: 21968653.
17. Khedri, Z; Li, Y.; Cao, H.; Qu, J.; Yu, H.; Muthana, M.; *Chen, X. Synthesis of selective
inhibitors against V. cholerae sialidase and human cytosolic sialidase NEU2. Org. Biomol.
Chem. 2012, 10, 6112-6120.
18. Khedri, Z; Muthana, M.; Li, Y.; Muthana, S.; Yu, H.; Cao, H.; *Chen, X. Probe sialidase
substrate specificity using chemoenzymatically synthesized sialosides containing
C9-modified sialic acid. Chem Comm. 2012, 48 (27), 3357-3359.
19. Song, X.; Yu, H.; Chen, X.; Lasanajak, Y.; Tappert, M.; Air, G.; Tiwari, V.; Cao, H.;
20.
21.
22.
23.
24.
Chokhawala, H.; Zheng, H.; *Cummings. R.; *Smith, D. A sialylated glycan
microarray reveals novel interactions of modified sialic acids with proteins and
viruses. J. Biol. Chem. 2011, 286 (36), 31610-31622.
Li, Y.; Yu, H.; Chen, Y.; Lau, K.; Cai, L.; Cao, H.; Tiwari, V. K.; Qu, J.; Thon, V.; Wang,
P. G.; *Chen, X. Substrate promiscuity of N-acetylhexosamine 1-kinases. Molecules 2011,
16, 6396-6407. Special Issue "Enzyme-catalyzed reactions".
Yu, H.; Cao, H.; Tiwari, V. K.; Li, Y.; *Chen, X. Chemoenzymatic synthesis of
C8-modified sialic acids and related alpha 2-3- and alpha 2-6-linked sialosides. Bioorg.
Med. Chem. Lett. 2011, 21, 5037-5040. PMID: 21592790
Li, Y.; Cao, H.; Dao, N.; Luo, Z.; Yu, H.; Chen, Y.; Baumgarth, N.; Cardona, C.; *Chen,
X. High-throughput neuraminidase substrate specificity study of human and avian
influenza viruses. Virology 2011, 415, 12-19. PMID: 21501853.
Chen, G.-Y.; Chen, X.; King, S.; Cavassani, K. A.; Cheng, J.; Zheng, X.; Cao, H.; Yu, H.;
Qu, J.; Fang, D.; Wu, W.; Bai, X.-F.; Liu, J.-Q.; Woodiga, S. A.; Chen, C.; Sun, L.;
Hogaboam, C. M.; Kunkel, S. L.; *Zheng, P.; *Liu, Y. Amelioration of sepsis by
inhibiting sialidase-mediated disruption of CD24-Siglec G interaction. Nat. Biotechnol.
2011, 29, 428-435. PMID: 21478876.
Padler-Karavani, V.; Hurtado-Ziola, N.; Pu, M.; Yu, H.; Huang, S.; Muthana, S.;
Chokhawala, H. A.; Cao, H.; Secrest, P.; Friedmann-Morvinski, D.; Singer, O.; Ghaderi,
D.; Verma, I. M.; Liu, Y.-T.; Messer, K.; Chen, X., *Varki, A., Schwab, R. Human
xeno-autoantibodies against a non-human sialic acid serve as novel serum biomarkers and
immunotherapeutics in cancer. Cancer Res. 2011, 71, 3352-3363. PMID: 21505105
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25. Li, Y.; Cao, H.; Yu, H.; Chen, Y.; Lau, K.; Qu, J.; Thon, V.; Sugiarto, G. *Chen, X.
Identifying specific sialidase inhibitors for human cytosolic sialidase NEU2 by
substrate specificity studies. Mol. Biosyst. 2011, 7, 1060-1072, PMID: 21206954.
DOI: 10.1039/C0MB00244E.
26. Pham, T.; Gregg, C.; Karp, F.; Chow, R.; Padler-Karavani, V.; Cao, H.; Chen, X.;
Witztum, J. L.; Varki, N. M.; *Varki, A. Evidence for a novel human-specific
xeno-auto-antibody response against vascular endothelium. Blood 2009. 114,
5225-5235, PMID: 19828701.
27. Cao, H.; Li, Y.; Lau, K.; Muthana, S.; Yu, H.; Cheng, J.; Chokhawala, H. A.;
Sugiarto, G.; Zhang, L.; *Chen, X. Sialidase substrate specificity studies using
chemoenzymatically synthesized sialosides containing C5-modified sialic acids.
Org. Biomol. Chem. 2009, 7, 5137-5145.
28. Cao, H.; Muthana, S.; Li, Y.; Cheng, J.; *Chen, X. Parallel chemoenzymatic
synthesis of sialosides containing a C5-diversified sialic acid. Bioorg. Med. Chem.
Lett. 2009, 19, 5869-5871. PMID: 19740656
29. Muthana, S.; Yu, H.; Cao, H.; Cheng, J.; *Chen, X. Chemoenzymatic synthesis of
a new class of macrocyclic oligosaccharides. J. Org. Chem. 2009, 74, 2928-2936.
Featured article. PMID: 19296596.
30. Muthana, S.; Cao, H.; *Chen, X. Recent progress in chemical and
chemoenzymatic synthesis of carbohydrates. Invited review. Curr. Opin. Chem.
Biol. 2009, 13, 573-581. PMID: 19833544.
31. Padler-Karavani, V.; Yu, H.; Cao, H.; Chokhawala, H. A.; Karp, F.; Chen, X.; Varki,
N.; *Varki, A. Diversity in specificity, abundance and composition of anti-Neu5Gc
antibodies in normal humans: Implications for disease. Glycobiology 2008, 18,
818–830. PMID: 18669916. PMC2586336.
32. Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V. K.; Son, B., *Chen, X.
Pasteurella multocida sialic acid aldolase: a promising biocatalyst. Appl. Microbiol.
Biotech. 2008, 79, 963–970. PMID: 18521592. PMC2588431.
33. Cao, H.; Huang, S.; Cheng, J.; Li, Y.; Muthana, S.; Son, B.; *Chen, X. Chemical
preparation of sialyl Lewis x using an enzymatically synthesized sialoside building
block. Carbohydr. Res. 2008, 343, 2863–2869. PMID: 18639240.
34. Chokhawala, H. A.; Cao, H.; Yu, H.; *Chen, X. Enzymatic synthesis of fluorinated
mechanistic probes for sialidases and sialyltransferases. J. Am. Chem. Soc. 2007,
129, 10630-10631.
35. Lewis, A. L.; Cao, H.; Patel, S. K.; Diaz, S.; Ryan, W.; Carlin, A. F.; Thon, V.; Lewis,
W. G.; *Varki, A.; Chen, X.; Nizet, V. NeuA sialic acid O-acetylesterase activity
modulates O-acetylation of capsular polysaccharides in Group B Streptococcus. J.
Biol. Chem. 2007, 282, 27562–27571. PMID: 17646166. PMC2588433.
36. Ni, L.; Chokhawala, H. A.; Cao, H.; Henning, R.; Ng, L.; Huang, S.; Yu, H.; *Chen,
X.; *Fisher, A. J. Crystal structures of a Pasteurella multocida sialyltransferase
complexes with acceptor and donor analogs reveal substrate binding sites and
catalytic mechanism. Biochemistry 2007, 46, 6288–6298. PMID: 17487984.
37. Cao, H.; *Yu, B. Synthesis of a S-linked heparan sulfate trisaccharide as the
substrate mimic of heparanase. Tetrahedron Lett. 2005, 46, 4337-4340.
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38. Yu, B.; Yang, Z. Y.; Cao, H. One-Pot Glycosylation (OPG) for the chemical
synthesis of oligosaccharides. Curr. Org. Chem. 2005, 9, 179-194
39. Yang, Z. Y.; Cao, H.; Hu, J.; Shan, R. L.; *Yu, B. 12 Migration and concurrent
glycosidation of phenyl 1-thio--mannopyranosides via 2,3-O-cyclic dioxonium
intermediates. Tetrahedron 2003, 59, 249-254.
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