Predictive Toxicological Approach and High Throughput Screening for Nanosafety Assessment Zhaoxia Ji UC Center for Environmental Implications of Nanotechnology University of California, Los Angeles, USA Engineered nanomaterials (ENM) provide tremendous opportunities for enabling new technologies that could address urgent societal needs. However, there are concerns over potential environmental, health and safety impacts of these materials as a result of the novel physicochemical properties that may generate hazardous biological outcomes. In order to assess ENM hazard, reliable and reproducible screening approaches are needed. At UC Centre of Environmental Implications of Nanotechnology, we propose a predictive toxicological approach that utilizes mechanism-based high-throughput screening (HTS) in vitro to make predictions about the physicochemical properties of ENMs that may lead to the generation of pathology or disease outcomes in vivo. In this talk, I will review the tools required for establishing predictive toxicology paradigm and discuss some of the major injury paradigms that have emerged through the use of well characterized ENM libraries and mechanism-based HTS assays.