LB Broth
P1 buffer:TrisEDTARnase
P2 buffer:SDSNaOH
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.J.E.Turnbull
Assessor
Laine Wallace
Assessment Number*
JETLW3
(#2047)
Date of Assessment
18/6/2002
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Laine Wallace, Dr.J.E.Turnbull,K.Drummond,Z.Schofield,Dr.E.Yates, Dr,S Guimond,
Dr.Z.Huang, Dr.A.Powell, J.Henstock, Dr. T. Kinnunen
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
5th Floor Biosciences Tower
DNA Preparation from Bacteria ( Maxi prep with Qiagen kit .)
AMOUNT
max/stored
LB Broth
705ml
P1 buffer:
Tris
10ml
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
N/A
Irirtant
R36/37/38: Irritating to eyes,
respiratory system and skin
EDTA
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
Rnase
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
Harmful
R22 : Harmful if swallowed
R36/38 : Irritating to eyes and
skin
Corrosive
R35 : Causes severe burns
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
P2 buffer:
SDS
10ml
NaOH
P3 buffer:
KAc
10ml
HAZDAT NO***
BIOSCIENCESNO***
QBT :
750nM NaCl
10ml
Irritant
R36 : irritating to the eyes
50m MOPS
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
15%Isopropanol
Irritant/flamm
able
R11 : Highly Flammable
R67 : Vapours may cause
drowsiness and dizziness
0.15% Triton x-100
Harmful
R22 : Harmful if swallowed
R41 : Risk of serious damage
to eyes
Irritant
R36 : irritating to the eyes
50mM Mops
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
15% Isopropanol
Irritant/flamma
ble
QC :
1M NaCl
QF :
1.25M Nacl
60ml
R11 : Highly Flammable
R36 : Irritating to the eyes,
R67 : Vapours may cause
drowsiness and dizziness
15ml
Irritant
R36 : irritating to the eyes
50mM Tris-Cl
Harmful
R20/22: Harmful by
inhalation and if swallowed
R42 : May cause sensitisation
by inhalation
R41 : Risk of serious damage
to eyes
R36/37/38: Irritating to eyes,
respiratory system and skin
15% Isopropanol
Irritant/Flamm
able
R11 : Highly Flammable
R36 : irritating to the eyes
R67 : Vapours may cause
drowsiness and dizziness
Isopropanol
(2-Propanol)
10.5ml
Irritant
Flammable
R11 : Highly Flammable
R36 : irritating to the eyes
R67 : Vapours may cause
drowsiness and dizziness
Ethanol
5ml
Irritant
Highly
Flammable
R11 : Highly Flammable
5
INTENDED USE**
Give brief details and attach protocol/instructions
LB – growth media for bacteria
P1- Destabilises membranes via Chelation of divalent cations,Rnase removes RNA
P2 – Solubilises and denatures protien, seperates chromosomal strand DNA but not Plasmid DNA
P3 - Renaturation of plasmid DNA
Isopropanol – Precipitation of DNA
Ethanol – Washing of DNA
QBT – Equilibration Buffer
QC – Wash Buffer
QF – Elution Buffer
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
P1 – Risk of skin and eye contact by solution splashes
P2 – Risk of skin and eye contact by solution splashes
P3 – – Risk of skin and eye contact by solution splashes
QBT- – Risk of skin and eye contact by solution splashes, risk of vapour inhalation
QC - – Risk of skin and eye contact by solution splashes, risk of vapour inhalation
QF – – Risk of skin and eye contact by solution splashes, risk of vapour inhalation
Isopropanol –, – Risk of skin and eye contact by solution splashes. Flammable – vapour may travel towards
sources of ignition and flash back, risk of vapour inhalation.
Ethanol – Risk of skin and eye contact by solution splashes , Flammable- vapour may travel towards sources of
ignition and flash back. Risk of vapour inhalation
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Due to the nature of some of the chemicals listed in this assessment there is a small risk . But due to the small
amounts used and as long as measures layed out in section 8 are followed then the level of risk is acceptable.
Non of the chemicals used can be replaced with other “ less hazardous” reagents.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Gloves, lab coat, safety glasses to be worn
Be aware of and avoid sources of ignition
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
One to one initial training.
10
MONITORING
Performance of control measures,
N/A
Personal exposure
Health Surveillance
N/A
N/A
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All chemicals ,at amounts listed once decontaminated with 1%virkon can be safely disposed of down the sink
with copious amounts of water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
18/6/2007
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Extinguish all ignition sources . Due to small amounts of any individual chemicals used mopping up spills with
absorbant material ( tissue ) is sufficient.
Large spills of stock reagents of Ethanol and Isopropanol warrant evacuation. Use of self-contained breathing
apparatus, rubber boots and heavy duty chemical resistant rubber gloves. Spill should be mopped up with
absorbant material, swept up into a bag, held for specialist waste disposal by Departmental Stores.
Ventilate area and wash spill with diltue decon solution after material pick up is complete.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Contact with any reagent in this protocol warrants immediate cleaning of area with copious amounts of water..
Remove all contaminated clothing.
Seek medical advice.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Bacteria containing spillages should be cleaned up with 1% Virkon then a dilute decon solution.
Other spillages should be cleansed with dilute decon solution.
CONTACT
Dr.J.E.Turnbull
PHONE
47527
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.