Syllabus for Developmental Biology Laboratory
Biology 374 -- Fall 2005
Monday 3:15 - 6:05 Rei 401
Reading materials:
 Wright SJ (2005) A Photographic Atlas of Developmental Biology [PADB]
 Handouts [HO]
 Lab manual [LM]
 Developmental Biology ONLINE website [DBO]
o http://www.uoguelph.ca/zoology/devobio/dbindex.htm
Course Outline
The lab is divided into three sections:
Section 1
Descriptive development (Developmental Anatomy)
Section 2
Experimental development
Section 3
Independent project
Tentative Schedule
Lab
Date
9/05
Topic

Reading
Labor day
1
9/12




Model organisms
Fish
Frog
Sea squirt
[PADB]
Ch 1
Ch 2 p 9-11
2
9/19


Fertilization of Frog
embryos
UV and LiCl treatment
[PADB] p6365,
p 77-80
[LM]– protocol



Fix embryos
Cleavage slides
insect development
[PADB] p 1016, Ch 6
[DBO]cleavage
[LM]- pp14-17
fly cycle CD
3
9/26
Goals
Learn:
 Course requirements
 Rules of dev bio lab
 the anatomical terms and
planes of section
 why different organisms are
studied
 how to use dissecting
microscope
 how to stage embryos

experimental analysis of
frog embryos at different
conditions
Turn in:
 UV or LiCl embryos
 Fertilization/cleavage
write up

View insect development
4
10/03

Cell adhesion
10/10

Columbus day
5
10/17


Finish last weeks lab
Gastrula slides –chick,
starfish, frog
6
10/24

Manduca brain

Morphology of the four to
fourteen somite chick
embryo (24-34 hours)
Morphology of the 24-38
somite chick embryo (48 85 hours)
7
10/31

8
11/07

chick wet lab
9
11/14

More chick lab
10
11/21

Planaria regeneration
11/28

Check on planaria
[LM] pp20-23
[PADB]
pp 87-101


Turn in manduca write up
Observe aggregation in
sponges
Turn in:
 aggregation lab
 gastrula slide ID sheet
[HO]- to be
handed out
[PADB]
pp116-123,
pp184-225

Turn in manduca write up


turn in chick ID
Observe chick embryo
development
Learn to remove and
manipulate embryo
HO – protocol
Ch 7
DBO- the
chick embryo

[HO]
[VM]
flatworms
Grades:
There are 9 lab write ups/ questions sets and 6 prelabs that that must be turned in. The write ups are
worth 25 points each and the pre-labs worth 5 points. This is a total of 255 (225+30). You will also be
given 25 points for in class participation for a total of 280 points.