BERGEN COMMUNITY COLLEGE
DIVISION OF MATH, SCIENCE AND TECHNOLOGY
Department of Biology and Horticulture
Student Course Outline
SEMESTER: Fall 2014
Instructor: John V. Smalley
Telephone: (201)-879-3622
Email: jsmalley@bergen.edu
Website: http://ww3.bergen.edu/faculty/jsmalley
Office# S-215
Office Hours: Monday: 2:00 P.M. to 3:00 P.M.
Wednesday: 4:45 P.M. to 5:45 P.M.
Thursday: 2:00 P.M. to 3:00 P.M.
----------------------------------------------------------------------COURSE TITLE:
BIO 202 Embryology
COURSE COORDINATOR:
Professor John V. Smalley
PREREQUISITES:
Any College Level Biology Course
COURSE CREDITS/HOURS:
4 Credits/3 Lecture, 3 Lab
GENERAL EDUCATION:
No
COURSE DESCRIPTION:
Embryology is the study of embryonic development
from gametogenesis and fertilization to the
development of organs, organ systems, and whole
organisms. Additional topics include regeneration
TEXTBOOK:
LABORATORY MANUAL:
and differentiation at both the cellular and
tissue level. Laboratory exercises include
experiments with living sea urchins, Japanese
Medaka fish, Zebra fish, Drosophila, Planaria,
Hydra, Dictyostelium, and carrot, as well as
microscopic examination of serial sections which
illustrate the embryonic development of selected
model organisms.
Principles of Developmental Biology, Fred H. Wilt
& Sarah C. Hake, Norton Publishing
ISBN 0-393-97430-8
A Photographic Atlas of Developmental Biology,
Shirley J. Wright, Morton Publishing Company
ISBN 0-89582-629-1
STUDENT LEARNING OBJECTIVES
The student will be able to:
1.
Identify the process and investigate the
critical events of embryonic development.
2.
Identify the origin and analyze the function of sex
cells.
3.
Distinguish the basic principles of embryonic
development.
4.
5.
6.
Survey the embryonic origin and development of various
body organs.
Summarize the genetic, biochemical and physiological
events of the embryonic development.
Demonstrate the methods and techniques employed in
embryological research using living material.
Lecture CONTENT:
Chapter
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
Page
Overview of Development
Gametogenesis, Fertilization and Lineage Tracing
Oogenesis and early development of Drosophila
Amphibian Development
Amniote Development
Development of Ectodermal Derivatives in Vertebrates
Development of Mesodermal and Endodermal Derivates in Verts
Metamorphosis
Plant Meristems
Reproduction in Plants
Cell Specification and Signaling in Plants
Cellular Associations, Environments, and Behaviors
Tissue Interactions and Morphogenesis
The levels of Regulation of Gene Expression in Development
Development Regulatory Networks I
Development Regulatory Networks II
Evolution and Development
3
17
39
59
79
101
129
159
177
192
209
231
253
279
302
334
363
LABORATORY SCHEDULE*:
*Laboratory schedule subject to change due to availability of living
organisms.
1.
Introduction, Basic principles governing embryonic development
(computer simulations).
Observations of Dictyostelium (living material).
2.
Grasshopper spermatogenesis and Ascaris oogenesis, maturation,
fertilization and development (slides).
Arbacia Starfish Development (slides).
Set up Spongilla aggregation experiment (living material).
LABORATORY SCHEDULE*:
*Laboratory schedule subject to change due to availability of living
organisms.
3.
Sea urchin Fertilization (living material).
Set up carrot callus experiment for continued observation
throughout the semester (living material).
4.
Drosophila Life Cycle and examination of developmental stages
(living material). Gel electrophoresis of proteins from various
developmental stages of Drosophila (living material).
Examination of Drosophila antennapedia mutants (living material).
5.
Japanese Medaka Fish Development (living material) and
observation of development throughout the semester.
6.
Frog Development - Testes, ovary, cleavage and blastula
slides). Frog Development - Gastrula and neurulation (slides).
Frog Development hatching stage (slides).
7.
Frog Development - 5-7 mm Tadpole(slides)
Frog Development - 10 mm Tadpole (slides).
8.
Set up carrot callus differentiation experiment (living
material).
Examination of Spongilla aggregation experiment (living
material).
9.
Introduction to Hydra.
(living material).
Set up Hydra regeneration experiment
10.
Introduction to Planaria. Set up Planaria regeneration
experiment(living material).
11.
Chick Embryo- ovary, spermatogenesis, 13-16 hour chick embryo
(slides).
Chick Embryo - 18 hour, 20-22 hour and 33 hour embryo (slides)
12.
Chick Embryo - 24-48 hour embryo (slides)
Chick Embryo - 56-72 hours embryo (slides)
13.
Chick Embryo - 96 hour embryo (slides).
14.
Examination of results of Hydra and Planaria regeneration
experiments (living material).
15.
Examination of carrot clones (living material).
EVALUATION:
A.
Unit Examinations. . . . . . . . . . . . . . .
55
%
B.
Laboratory Work
. . . . . . . . . . . . . . .
30
%
C.
Report/Project . . . . . . . . . . . . . . . .
10
%
D.
Class Participation
5
%
E.
. . . . . . . . . . . . .
Other . . . . . . . . . . . . . . . . . . . .
TOTAL . . . . . . . . . . . . . . . . . . . .
%
100%
CLASS PROTOCOL
Make-up Testing: Any student who misses an exam without prior arangements will be required to take a
cumulative final at the end of the semester to replace the missed grade. Any missed lab quiz will result in a zero
unless prior argangments have been made. In either case if an exam or quiz is missed due to an emergency a
make-up will be permited if the student can provide WRITTEN documentation to confirm the nature of the
emergency. Once the first student has left the exam NO students will be allowed to start the exam.
Class Assignments Late assignments will not be accepted unless prior arrangements have been made.
Class Attendance: There is no attendance policy for lecture classes. Success in class is related to attendance
and it is hoped that the students will attend classes. If you miss a class it is YOUR responsibilty to find out
what you missed either from a classmate or from me. Absence is no excuse.
Lab Attendance: If you find it necessary to miss a laboratory meeting, you should make arrangements, upon
your return to school, to make up the laboratory class. There is no make-up for missed quizzes unless prior
arrangements have been made. If you miss a Lab it is YOUR responsibilty to find out what you missed either
from a classmate or from me. Absence is no excuse.
Lateness: Lateness to class or lab sometimes cannot be avoided. Continued lateness is frowned upon.
Smoking Policy: As of January 1, 1992, Bergen Community College facilities are smoke free. Smoking is not
allowed in any building on campus.
Eating & Drinking: Eating and drinking in the classrooms, lecture halls, laboratories and passageways is
forbidden. Eating and drinking are permitted in the cafeteria and vending areas only.
Faculty Absence: If a class finds the instructor is absent, a class representative should report to Divisional
Office(A325) or the evening office (L-113) after ten minutes of the period have elapsed. The class should
remain until the representative returns with instructions. If class has been cancelled whatever we were going to
do that day will be done at the next meeting time and the students should sign in and slip the sign in sheet under
my door S-230
Laboratory Safety: Your laboratory instructor will review safety precautions prior to each laboratory session.
Careful adherence to these precautions is essential in order to prevent injury to yourself or to others working
around you.
BS202sco.fa10
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----------------------------------------------------------------------I have carefully read and fully understand the addenda.
Signed
Date