METHOD VALIDATION FOR THE ANALYSIS OF PHARMACEUTICALS AND PERSONAL CARE PRODUCTS IN BIOLOGICAL TISSUES Platform Presentation Prepared by C. Hamilton and B. Chandramouli AXYS Analytical Services Ltd. 2045 Mills Road, Sidney, BS V8L 5X2 Canada Contact Information: [email protected]; 250-655-5802 Abstract A method for the analysis of biological tissues for 104 PPCP compounds has been developed and validated. The method is based on EPA Method 1694A but also includes the additional 32 compounds that are being added in an upcoming revision of this method (Method 1694B). The tissue method incorporates all aspects of the LC/MS/MS analysis, quantification and QA/QC but the extraction has been modified to accommodate the tissue matrix. In this presentation we will discuss various extraction and extract cleanup options that were investigated, the effects of extraction solvent and sample size. Method performance data for the finalized method will be presented along with typical data for the analysis of fish and mussel samples.