AP Biology Final exam SG Semester 1 2011 Biochemistry: Nucleotides: Building block of nucleic acids, made of a 5 carbon sugar, phosphate group and a nitrogen base pH: measure of the concentration of H+ ions, each value is 10x smaller that the previous value; the more H+ ions the stronger the acid why is water cohesive: H bonds form between the oxygen end of 1 water molecule and the hydrogen end of another; separation of charges is because oxygen is more electronegative energy source for cells: hydrolysis of ATP how can you determine the rate of enzyme reactions: measure the disappearance of the substrate or the production of the product what is the C source for plants: carbon dioxide taken in and “fixed” in photosynthesis proteins: made of chains of amino acids at the ribosome carbohydrates: made of glucose units; monosaccharides, disaccharides, polysaccharides (cellulose, chitin, glycogen) nucleic acids: carry the genetic code lipids: made of glycerol and fatty acids, used for insulation and long term energy storage steroids: lipids that are used to make cholesterol and hormones coenzymes: non-protein helper molecules, needed for some enzymatic reactions to take place competitive inhibitors: block the active site of an enzyme characteristics of enzymes: made of chains of amino acids, specific, not used up, have optimal temperature and pH ranges, decreases the activation energy to start a reaction, combine with a substrate to help a reaction Cells: endosymbiotic theory: mitochondria and chloroplasts used to be free living organisms; both have their own DNA prokaryotes vs eukaryotes: P: no nuclear membrane or membrane organelles, single circular chromosome, E: has a nucleus and membrane organelles; both have DNA and ribosomes chloroplast: site of photosynthesis golgi: packages proteins for transport nucleus: contains the DNA nucleolus: makes ribosomes lysosomes: contains digestive enzymes mitochondria: site of cellular respiration ER: tranports proteins in the cell Ribosomes: make proteins Tonoplast: membrane that surrounds the central vacuole Osmosis: movement of water from a high concentration to a low concentration active transport: movement from a low concentration to a high concentration; requires energy facilitated diffusion: movement from high to low using a transport protein, a type of passive transport plasmodesmata: pores in the cell wall that allow plant cells to communicate with each other tight junctions: connections between animal cells that don’t allow passage of materials desmosomes: anchoring intercellular junction between animal cells osmotic pressure: the higher the solute concentration the higher the osmotic pressure animal vs plant cells: plant cells have a large central vacuole and cell walls difference between cancer cells and normal cells: cancer cells have a shorter cell cycle and can stop at any place; no control mechanisms hypertonic: solution that has more solutes (less water) than another; cells will shrink in this solution hypotonic: solution that has less solutes (more water) than another; water always goes hypo to hyper; cells will swell in this solution isotonic: equal concentration of solutes; no net movement of water or change in cells Photosynthesis and Cellular Respiration: Light reactions vs Calvin Cycle: ATP and NADPH are made in the light reactions and used in the Calvin Cycle end products of light reactions: light energy is captured by pigment molecules and the energy from the excited electrons is stored as ATP and NADPH; some of the energy is used to split water to release oxygen enzymes in Calvin Cycle: Rubisco fixes carbon from carbon dioxide C3 vs C4 plants: C4 plants are better at “fixing” carbon since they have to store it until the plant is ready for photosynthesis reactants and products of each set of reactions—where do the products come from: Light reactions: use water, produce ATP, NADPH, and oxygen (comes from the water); Calvin cycle: uses carbon dioxide, ATP, NADPH, produces glucose best wavelength light for photosynthesis: red light chloroplast vs mitochondria: both have DNA, carry out chemiosmosis to make ATP, are double membrane organelles, ways to increase rate of photosynthesis: increase carbon dioxide levels, use optimal temperature, increase stomata number, increase # of enzymes glycolysis: occurs in the cytoplasm, splits glucose into 2 pyruvate molecules, produces 2 ATP, 2 NADH and 2 pyruvate molecules, uses the energy from 2 ATP to get started Krebs cycle (Citric Acid cycle): occurs in the mitochondria; uses acetyl CoA to produce 2CO2, ATP, NADH and FADH2 ETC: occurs in the inner mitochondrial matrix, makes 32-34 ATP per glucose Chemiosmosis: uses a H+ ion concentration gradient to make ATP, occurs in the mitochondria and chloroplast Cytochromes: proteins in the electron transport chain in both photosynthesis and cellular respiration FADH2: carries electrons to the ETC in Cellular Respiration; adds them at a lower energy level than NADH NAD+: picks up electrons and H ions to form NADH in Cellular Respiration and carries them to the ETC; drops them off at the top of the ETC NADP+: picks up electrons and H ions to form NADPH in photosynthesis; used to power the Calvin cycle oxygens role in cellular respiration: final electron acceptor to form water; most ATP is made if oxygen is present Cell respiration—steps: Glycolysis, Krebs cycle, ETC reactants and products of each: see above answers why/what forms most ATP in CR: chemiosmosis and ETC substrate level phosphorylation: adds P from molecules; forms ATP in glycolysis and Krebs Cycle pyruvic acid: same as pyruvate, formed in glycolysis acetyl CoA: 2 carbon fragment that enters the Krebs cycle proton gradient: concentration difference of H+ ions across a membrane, when they even out they move through ATP synthase and make ATP why is ATP a good energy source: its energy is easily stored and released ATP structure: ribose, adenine, 3 phosphate groups Fermentation (anaerobic respiration): produces only 2 ATP (those produced in glycolysis), products can enter aerobic respiration when oxygen becomes available; 2 types: alcoholic fermentation and lactic acid fermentation Genetics: polygenic inheritance: controlled by many genes, examples are eye color and skin color in humans genetic problems: monohybrid, dihybrid, sex-linked, blood types, fruit flies: see the practice genetics problems page probability rules: having a boy = ½, having 2 boys in a row = ½ x ½ = ¼ testcross: allows you to determine the genotype of an organism; mating an organism with a dominant phenotype but unknown genotype with a homozygous recessive organism; if any recessive offspring are produced the parent is heterozygous dominant vs recessive: if you get 1 dominant gene you show the dominant trait; you need 2 recessive alleles to show the recessive trait linkage: genes are found on the same chromosome epistasis: 1 gene influences the expression of another; usually a 9:3:4 ratio in the offspring locus: location of a gene on a chromosome Evolution: heterozygote advantage: heterozygote is better off than either of the homozygotes; example: sickle cell anemia—heterozygote is resistant to malaria but doesn’t show the symptoms of sickle cell punctuated equilibrium: long periods of no evolutionary change followed by periods of rapid change gradualism: slow steady change in a species over time species: group of organisms that can mate and produce fertile offspring under natural conditions Hardy-Weinberg problems: see lab #8 why are hybrid organisms sterile: hybrids are normally sterile due to chromosome differences convergent evolution: organisms that are not closely related evolve similar structures because they are advantageous in their environment; for example—fins/flippers in dolphins, fish and penguins divergent evolution: speciation reproductive isolation: keeps gene pools separate and allows species to become or remain separate temporal isolation: differences in timing of reproduction habitat isolation: live in the same place but don’t meet so cant mate behavioral isolation: species-specific mating rituals geographic isolation: populations are separated by a geological formation (mountains, streams, etc) that keep their gene pools separate; can lead to allopatric speciation adaptive radiation: a population can evolve into many different species in a relatively short period of time given enough variation in the population and time Hardy-Weinberg conditions: large population, no mutation, no migration, equal reproductive success, random mating Microevolution: change in allele frequency of a population over time homologous structures: similar structures in closely related organisms; examples—forelimbs of mammals feeding response: a mother bird regurgitates her meal at the sight of her offspring altruism: aiding another organism at your own expense Kin selection: altruism among related organisms genetic drift: change in allele frequency due to random chance; affects a small population more than a large one polyploidy: containing more than 2 sets of chromosomes; can explain some speciation events in plants allopatric speciation: speciation when groups are geographically separated from each other ,sympatric speciation: speciation without geographic separation Mutation: change in the DNA sequence of an organism sexual selection: female selection for the best genes in her mate; can lead to extreme forms in the males like in peacocks Natural Selection—statements: variation exists in organisms, there is competition for resources, more individuals are born than can survive, evolution takes long periods of time, individuals best adapted to their environment survive, reproduce and pass on their genes to the next generation gene flow: movement of genes into or out of a population bottleneck event: most organisms in a population die, those that are left may not have the same allele frequency as the original population but they are the only ones to create the next generation hybrid sterility: there are barriers to reproduction between different species Plants: Mesophyll: middle layer of the leaf, site of photosynthesis Transpiration: evaporation of water from the stomates, with cohesion allows water to move to the top of trees angiosperm reproduction—microspore: male gametophyte, becomes the pollen grain megaspore: produces the female gametophyte (embryo sac) gametophytes: haploid structure that produces gametes why are angiosperms so successful: don’t need water for fertilization, produce flowers for pollination, produce fruit for seed dispersal endosperm: triploid food in seeds for embryonic plants hypocotyl: in dicots the first part of the stem to be visible above ground ovary: contains ovules, becomes the fruit auxin (IAA): hormone that is responsible for elongation of shoots and apical dominance in plants gibberellins: hormone that is responsible for germination cytokinens: hormone responsible for cell division phytochromes: protein isomers that control flowering in short day plants ethylene: hormone that controls ripening of fruit, only hormone that is a gas what controls flowering in short day plants: see above answer double fertilization: in angiosperms 1 sperm nucleus fertilizes the egg, the other fertilizes the diploid cell to become the triploid endosperm pollen tubes: produced by the pollen tube nucleus, allows the sperm nuclei to reach the embryo sac fruit function: seed dispersal root hairs: increase surface area for absorption of water and minerals Casparian strip: controls entry of substances into the vascular cylinder of a root root cap: protects the apical meristem zone of maturation: plant takes on its adult function here apical meristem: produces new plant cells for the life of a plant, allows a plant to have indeterminant growth translocation: movement of sugars in plant phloem vessels companion cells: found outside sieve tube element cells, controls their functions tracheids: xylem vessel cells, dead at functional maturity trichomes: small hairs on a plant that help control water loss and prevent herbivory sieve tube elements: phloem vessel cells, are alive at maturity vessel elements: xylem vessel cells cohesion: attraction of water to other water molecules, contributes to water movement up a plant root pressure: movement of water a short distance into a root capillary action: movement of water a short distance into a narrow tube why do stems bend toward the light: auxin causes elongation of the cells in the dark why does sugar move: pressure and osmotic differences between the source and the sink results of stomates closing: reduction in CO2 levels in the plant so a decrease in photosynthetic rate parenchyma: general plant cell, gives rise to all other types sclerenchyma: forms xylem vessels, contains lignin for extra support of the plant collenchyma: support cells in young plants xylem: transports water and minerals phloem: transports sugars what factors increase transpiration: heat, wind, dry conditions gymnosperms: “naked” seed plants, conifers mosses: gametophyte generation is dominant, produces a sporophyte consisting of a stalk and a capsule containing a sporangium ferns: most primitive vascular plant algae: water-dwelling autotrophs