Biotechnology Lab Topic Schedule
The following schedule will serve as a general guideline; some lab sections may be moved.
Quarter 1
Week Date
1
8/21-24
Source
Lab Manuel 1A
and 1B
2
8/27-31
Lab Manuel 4E
& 4F
3
9/4-7
4
9/10-14
5
9/18-21
6
9/24-28
Lab Manuel
2B
Lab Manuel
3A & 3B
Lab Manuel
3D
Lecture
Lab Manuel 2E
7
8
10/1-5
10/8-12
(Magic
Mtn)
10/15-19
9
10/22-26
Lab Lesson Focus
Scientific Notebook
Laboratory Safety
MSDS
Media Prep
Sterile Technique
Bacteria Cell Culture
Model Organisms
Pipeting
Micropipeting
Checking Accuracy
Pipet Skill Test
The Cell (Structures, Functions,
Cycle)
Organic Chemistry
Lectures
DNA/RNA
Lab Manuel 4A, Isolating DNA, DNA spooling,
4B, 4C, 4D
Testing for DNA, RNA & Protein,
Quick DNA test
Electrophoresis Lecture
Loading Gel Practice
Lecture Polymerase Chain Reaction
Quarter 1 Exam
Fall Break – End of Quarter 1
Text Reading
Biotechnology Book Chapter 1
Biotechnology Book Chapter 4
Biotechnology Book Chapter 2
Biotechnology Book Chapter 3
Biotechnology Book Chapter 3
Biotechnology Book Chapter 2
Biotechnology Book Chapter 4
Biotechnology Book Chapter 4
Biotechnology Book Chapter 4
Quarter 2
Week Date
10
10/2911/2
11
11/5-9
12
11/13-16
13
11/19-20
Source
BIO-RAD
PV92 PCR Informatics Kit
BIO-RAD
Lambda DNA Kit &
AP Bio Lab 6B
BIO-RAD
Forensic DNA Fingerprinting Kit
Lab Lesson Focus
DNA goes to the Races
mtDNA isolation, PCR, Electrophoresis,
Hardy-Weinberg, Bioinformatics
DNA Scissors Activity
Restriction Enzymes, Electrophoresis, standard
curve
Restriction Enzymes, Electrophoresis
Text Reading
Biotechnology Book
Chapter 13
Thanks-giving
14
11/26-30
15
12/3-7
16
12/1012/14
17
12/17-19
17
12/20—
1/2
1/3-4
18
1/7-11
19
1/14-18
20
1/22-25
BIO-RAD
GMO Investigator Kit
Carolina SNPs
BIO-RAD
Crime Scene Investigator PCR
Basics Kit
Carolina Handouts
EZ Gene Splicer
Genetically Modified Food, DNA isolation,
PCR, Electrophoresis, Bioinformatics
Single Nucleotide Polymorphorism, DNA
isolation, PCR, Electrophoresis
Short Tandem Repeats (STR), PCR,
Electrophoresis
Ligation, Transformation
Winter Break
Lab Manual 13a, b, c, d or
KIT???
Lab Manual 13a, b, c, d or
KIT???
BIO-RAD
pGLO Bacterial Transformation
Kit & Green Fluorescent Protein
Chromatography Kit
BIO-RAD
pGLO Kit SDS-PAGE Extension
Southern Blot
Southern Blot
Transformation
Biotechnology Book
Chapter 8
Purification Protein, chromatography
PAGE
Biotechnology Book
Chapter 9
Quarter 3
Week Date
21
1/29-2/1
22
2/5-8
23
2/11-14
24
2/19-22
25
2/25-3/1
26
3/4-8
27
3/11-15
28
3/18-22
29
3/25-28
4/1-4/5
Source
Carolina Fish
Protein
Fingerprinting
on
Polyacrylamide
Gels
Coastal Marine
Biolabs
Coastal Marine
Biolabs
Coastal Marine
Biolabs
Coastal Marine
Biolabs
Coastal Marine
Biolabs
Coastal Marine
Biolabs
Lab Manual 2F
AP Bio Lab 2
Lab Lesson Focus
PAGE, analyzing proteins, evolution
Ecotechnology: Barcoding Life’s
Matrix
Ecotechnology: Barcoding Life’s
Matrix
Ecotechnology: Barcoding Life’s
Matrix
Ecotechnology: Barcoding Life’s
Matrix
Ecotechnology: Barcoding Life’s
Matrix
Ecotechnology: Barcoding Life’s
Matrix
Lectures: Protein Folding, Enzymes
Protein Folding
Enzyme Catalysis
Spring Break
Text Reading
Biotechnology Book Chapter 5
Biotechnology Book Chapter 2
Quarter 4
Week Date
30
4/8-12
31
4/15-19
32
4/22-26
33
4/29-5/3
(testing)
34
35
5/6-10
(testing)
5/13-17
36
5/20-24
Source
Lab Manual 5AG
Lab Manual 5AG
Lab Manual 5AG
Carolina
Assay for
Antibiotic
Resistance
Lab Manual
7A--I
Lab Manual
7A--I
Lab Manual 14B
Lab Lesson Focus
Enzymes, Presence of Proteins, PAGE
Text Reading
Biotechnology Book Chapter 5
Enzymes, Presence of Proteins, PAGE
Biotechnology Book Chapter 5
Enzymes, Presence of Proteins, PAGE
Biotechnology Book Chapter 5
37
5/28- 31
Lab Manual 14A ELISA
38
6/3-7
Color Assays, pH
Spectrophotometer, measuring pH,
Buffer Efficacy, Assay Techniques
Spectrophotometer, measuring pH,
Buffer Efficacy, Assay Techniques
Western Blot
Graduation Practice 