Microbiology 2 Bacteria that can only live with or in the presence of free oxygen from the air. They require oxygen for respiration. aerobic bacteria Bacteria (micro-organisms) that can or must live in the absence of free oxygen from the air. anaerobic bacteria An agent that inhibits growth of micro-organisms but does not kill those already growing. antiseptic Methods used to prevent unwanted micro-organisms entering an area of an experiment. asepsis or aseptic techniques Free from all pathogens, i.e. organisms that cause disease. aseptic Bacteria that are self-nourishing, i.e. capable of making (synthesising) their own food from inorganic compounds. autotrophic bacteria Microscopic unicellular organisms, prokaryotic in nature. May be parasites or saprophytes. Some are autotrophic. Many cause disease or decay and some are used in industry. They are ubiquitous. Are composed of cell wall, cell membrane, genetic/nuclear material – DNA (not in a nucleus), plasmids, cytoplasm, flagella and capsule. bacterium (bacteria) A virus that attacks and kills bacterial cells. It is composed of DNA and protein only. bacteriophage A container in which a living thing is used in the production of something useful, e.g. during fermentation using yeast, yogurt production using bacteria, etc. A method of asexual reproduction where a new individual arises from an outgrowth that may or may not separate to form the parent, e.g. in yeast. This outgrowth may remain attached to the parent and form a colony or it may detach from the parent, undergo further reproduction and form a new colony itself. bioreactor budding Protective mucilage coat surrounding certain bacteria. capsule Human disease caused by a virus. Symptoms: red spots or lumps which eventually turn into scabs, sore throat and tiredness. chicken pox Tiny, hair-like cytoplasmic extensions of a cell which beat rhythmically. In lower animals they are for locomotion; in higher forms, for propulsion of substances, e.g. mucus from the trachea. cilia (cilium) A wall separating the sporangium from the sporangiophore of a reproducing Rhizopus (bread mould). columella Page 1 of 4 The fourth phase in a growth curve. Numbers decline due to increased competition for food and space and the build-up of toxic wastes. Unhealthy condition in which an organism or part of an organism is affected by some factor which interferes with normal growth, development or metabolism of its organs or tissues. A spore formed within a parent cell, e.g. bacterial spores. Formed in unfavourable conditions. Cell contents shrink and enclosed by a thick wall. When suitable conditions return the spore absorbs water, wall bursts and the spores divide by binary fission. death phase / decline phase disease endospore Whip-like process or appendage of a cell, e.g. bacteria. Used for propulsion. flagellum (flagella) An asexual method of reproduction where parts of an organism break off and are capable of developing into a new organism, e.g. seaweeds, bacteria, fungi (yeast). fragmentation Structures involved in reproduction in fungi (mushrooms) that produce spores. fruiting bodies Plant that has no stamens or pistils (hence no proper flowers or seeds) and no chlorophyll. Eukaryotic in nature. Feeds on organic matter. Found in a wide variety of habitats, e.g. salt water, fresh water, terrestrial, air-borne, e.g. mushrooms, yeasts, toadstools and moulds (Rhizopus). fungus (fungi) Common name for micro-organism. OR something capable of developing into a new organism, e.g. embryo. germ Graph showing population size of micro-organisms over a period of time. Has five phases: lag phase, log phase, stationary phase, death/decline phase, and survival phase. growth curve Bacteria that cannot make their own food. They may be saprophytic or parasitic. heterotrophic (bacteria) The capacity of an organism (the host) to withstand the action or effects of a pathogen or other chemicals or disease. host resistance Cause to develop/grow by supplying all the necessary requirements, e.g. temperature (using an oven), food (as a nutrient medium in the incubate case of bacteria), etc. A protein substance produced by cells which have been infected by a virus. It has the effect of stopping virus growth and protecting surrounding cells against viral infection. Can be produced by genetic interferon engineering and is used to treat a variety of conditions (hepatitis B and C) and some cancers. Particle or organism large enough to be visible to the naked eye. Page 2 of 4 macroscopic Organism, usually bacterium or virus, not visible to the naked eye. micro-organism Collective term for all the hyphal filaments that make up the vegetative part of a fungus. mycelium (mycelia) Symbiotic relationship between a fungus and plant roots. The mycelium forms a web over the surface of the roots (exotrophic mycorrhiza) and their hyphae enter the roots (endotrophic mycorrhiza). mycorrhiza An organism that causes a disease. pathogen Shallow round dish with lid into which is placed a nutrient medium for growing micro-organisms. petri-dish An infectious agent made of one or more protein molecules but no RNA or DNA. They seem to act as catalysts – converting normal protein into prions, e.g. BSE and CJD. prion Component of a virus surrounding one type of nucleic acid. protein coat A root-like filament which attaches the mycelium of some fungi to the substrate. Grows downwards into substrate. It functions like a root – it supports the fungus and is involved in absorption. Classification grouping of bacteria called Bacillus. Any bacteria called Bacillus ~ or B. ~ have this shape; also E. coli, found in the human colon, is similarly shaped and is responsible for the synthesis of some B vitamins. Also: cells in retina of eye stimulated by low light intensity, i.e. dim light. Responsible for monochromatic vision (can see in black and white or shades of grey). rhizoid rod(s) Term referring to fungal hypha with cross-walls. Has cells. septate A structure in which spores are produced, e.g. in Rhizopus. sporangium (sporangia) The third phase in a growth curve. Birth rate = death rate due to competition for food, space and the build-up of toxic wastes. stationary phase Fungal hyphae which grow horizontally on surface of substrate (e.g. bread). stolon The final phase in a growth curve. Growth settles at a level that the environment can support or in the case of bacteria, endospores may be formed due to the adverse conditions. survival phase Page 3 of 4 Noxious biochemical substance (poison of animal or vegetable origin) formed in the body by a pathogenic organism. toxin The infectious part of a virus consisting of the RNA or DNA and the protein coat. virion Unicellular fungus, ovoid or spherical cells found singly or in groups (e.g. on fruit skins such as apple and grape). Thin cell wall, granular cytoplasm, nucleus and storage vacuoles. Reproduce by a process called budding. yeast Page 4 of 4