CONCERN
NANOINDUSTRY
20, building 4, Nauchnyy passage, Moscow 117246, Russia.
E-mail: nanotech@nanotech.ru Tel. +7 (495) 332-88-11, 332-88-22, 332-88-33.,
http://www.nanotech.ru
RESULTS OF STUDYING BIOCIDAL ACTIVITY
OF AgBion-2 PREPARATION BASED ON SILVER NANOPARTICLES
AND ITS POTENTIAL DANGER FOR HUMAN
Complex studies of materials and products developed in Russia and
manufactured using nanoparticles of various natures are carried out in several
authoritative scientific and medical organization. The studies are fulfilled for the
purpose of detecting possible application areas of these products as well as their
potential danger for human health and environment.
AgBion-2 colloidal water solution of silver nanoparticles (Technical
specifications 2499-003-44471019-2006) developed in INAT ICF was one of study
object. The way of obtaining colloidal solution of silver nanoparticles is protected by
patent of Russian Federation.
Sanitary-microbiological estimation of biocidal effect of materials used
for ballet shoes manufacturing and modified with working solution based on
"AgBion-2" preparation upon the following microorganisms: E.coli (ATCC
11229), Pseudomonas aeruginisae (ATCC 15442), Staphylococcus aureus,
Bacillus Subtilis, Candida albicans has been carried out at Test Laboratory
Centre of A.N. Sysin R&D Institute of Human Ecology and Environment
Hygiene
of
Russian
Academy
of
Medical
Sciences (Certificate
№ГСЭН.RU.ЦОА.234). Results of experimental studies demonstrated that cheap
cotton goods samples as well as two-layer material samples modified with
working solution based on "AgBion-2" preparation produce pronounced biocidal
effect upon a wide range of microorganisms. It makes it possible to recommend
using this preparation as an active inactivating agent against pathogenic micro
flora in various environment objects including modification of materials applied for
ballet shoes manufacturing.
Biocidal effect of preparations based on "AgБион-2" diluted at ratio of 10, 30,
100 and 300 times upon E.coli, Pseudomonas aeruginosa, RNA-containing phages
MS-2, Staphylococcus aureus, Bacillus subtilis and Candida albicans
microorganisms at their initial concentration in water of n=102/ml to 104/ml was
studied at the same centre.
It has been demonstrated that studied preparations based on "AgBion-2"
produce a pronounced biocidal effect upon a wide range of microorganisms. It
resulted in their inactivation at various intensity levels, from full inactivation to
reducing concentration of microorganisms by 10000 times.
Biological activity tests of AgBion-2 preparation as well of materials based on
it have been fulfilled in other scientific and medical organizations as well: N.F.
Gamaleya Institute of Epidemiology and Microbiology, Institute of Nutrition of the
Russian Academy of Medical Sciences, Central R&D Institute for Epidemiology of
Federal Service on Supervision in the Field of Consumer Rights and Human Wellbeing Protection, D.I.Ivanovsky Research Institute for Virology, State R&D Institute
of Restoration and in Institute of Solutions Chemistry of Russian Academy of
Sciences.
Antimicrobial effect of "AgBion-2" preparation upon the following
microorganisms of collection of N.F. Gamaleya Institute of Epidemiology and
Microbiology of Russian Academy of Medical Sciences and international ATCC
collection:
Staphylococcus aureus (ATCC 6538),
E.coli (ATCC 11229),
Pseudomonas aeruginisae (ATCC 15442), Klebsiella pneumoniae, Candida
albicans (ATCC 10231), Salmonella spp., Acinetobacter spp., Enterobacter spp.
has been tested at Test Laboratory "Finist" Self-governing Non-profit
Organization (certified test laboratory, certificate №РОСС.1Ш.001.2ШМ28) and
N.F. Gamaleya Institute of Epidemiological Medicine of Russian Academy of
Medical Sciences. Studies demonstrated that "AgBion-2" preparation produces
bactericidal effect upon all tested microorganisms taken in doses of 10 6, 104
and 102 colony-forming units.
Study of biocidal effect of water acryl paints and acryl paints based on organic
solvent modified with AgBion preparation at percentage of 1% upon the following
group of disease-producing microorganisms: Staphylococcus aureus ( ATCC 6538),
E.coli (ATCC 11229), Pseudomonas aeruginisae (ATCC 15442), Klebsiella
pneumoniae, Candida spp., Enterococcus spp., Bacillus spp. has been studied at the
same laboratory. Data obtained as the result of fulfilled studies confirm the full
elimination of mentioned microorganisms deposited at modified paints.
Studies on evaluation of application efficiency of AgBion-2 preparation for
ensuring biological activity of flax-containing materials have been carried out
at the Institute of Solutions Chemistry of Russian Academy of Sciences. Biological
activity of AgBion-2 preparation and cellulose materials treated with it was
estimated based on growth suppression zone of test cultures Staphylococcus and
E.coli at dose of 106 colony-forming units and more. Research results confirm high
biological activity of AgBion-2 preparation and possibility of its application for
manufacturing materials for medical purposes. Besides, mentioned preparation
reduces biodegradability of flax-containing materials and can be used for obtaining
biologically active flax fibers for technical purposes.
The State R&D Institute of Restoration has carried out tests of AgBion-2
preparation as a disinfection agent for treating cellar rooms and walls of
architectural monuments, infected by micromycetes: Penicillium species,
Aspergillus versicolor, Cladosporium sp., Sporotrichum sp. and Мycelia sterilia.
Studies were carried out at sarcophagi and walls in underground chambers of
Arkhangelsky Cathedral of Moscow Kremlin as well as at ground floor walls of a
XVIII century palace. It has been demonstrated that preparation consisting of
AgBion-2 concentrate diluted by water at ratio of 1:10 produces suppressing impact
upon mold fungi. Experimental sites remained not infected for almost two months.
Mold fungi concentration was 100 times lower to the end of the second month than
before treatment.
Studies fulfilled at the Central R&D Institute for Epidemiology of Federal
Service on Supervision in the Field of Consumer Rights and Human Well-being
Protection demonstrated that AgBion-2 preparation is an effective agent for
suppressing tuberculosis, dermatophytosis and mold fungi.
It has been demonstrated in D.I.Ivanovsky Research Institute for Virology that
AgBion-2 eliminates poliomyelitis and HIV-1BRU viruses.
Dynamics of silver emission from nonwoven material of ion-exchange fiber
treated with AgBion-2 preparation during water infiltrating through it was studied at
Sector of Applied Water Ecology of Institute of General and Non-organic Chemistry
of Russian Academy of Sciences. It has been demonstrated that studied materials
emit silver into water at rate up to 0,05 mg/l. This value meets requirements of
Sanitary Rules and Norms 2.1.4.1074-01 regarding silver percentage in potable
water. Studied materials ensure uniform silver emission into solution. It makes it
possible to use them as a component of bactericidal additives in water purification
systems in principle.
Expert conclusion provided by A.N. Sysin R&D Institute of Human
Ecology and Environment Hygiene of Russian Academy of Medical Sciences
recommends state registration of "AgBion-2" preparation as an agent for disinfecting
surfaces in various institutions disinfecting water in technical water supply
systems and recycled water in equipment cooling systems.
Toxicological characterization of "AgBion-2" preparation has been
fulfilled during experiments with laboratory animals in the Institute of Nutrition
of the Russian Academy of Medical Sciences. Studies were carried out at seven
groups of growing male rats of Vistar line. Materials to be tested were injected
into stomach with the help of stomach pump for 28 days. Data obtained in the
tests indicate the absence of pronounced negative impact of metallic silver
nanoparticles of AgBion-2 preparation upon state of health of laboratory animals.
Thus, positive conclusions of organizations mentioned above confirm
that colloidal solutions of nanoparticles at low concentration can be used as
disinfection agents of a wide effect spectrum at public accommodation
places and materials modified with "AgBion-2" preparation really have
high biocidal properties and are harmless for people.