SBI 4U/AP Ms. Girvan Grade 12/AP Biology 2014 Exam Review Unit #1 – Biochemistry (~ 22%) 1. Matter: Atoms (composition), Elements (atomic mass and number), Compounds, Microelements 2. Isotopes (radioisotopes) 3. Ions (cation and anion), Lewis-Dot diagrams, Bohr-Rutherford diagrams (valence shell electrons) 4. Electronegativity, bonding patterns (covalent, ionic, polar and non-polar covalent), polarity and partial charges 5. Molecular shape and polarity 6. Properties of water 7. Intermolecular bonds 8. Solubility of substances in water (hydrophilic vs. hydrophobic regions) 9. Acids, bases, and neutralization reactions 10. Buffers 11. Composition of organic molecules 12. Types of isomers (structural, geometric, enantiomers) 13. Functional groups 14. Types of chemical reactions (hydrolysis and dehydration synthesis) 15. Types of macromolecules (structure and function) and bonding patterns [carbohydrates, lipids, proteins, nucleic acids] 16. Be able to identify the structural diagrams of the following molecules: amino acid, steroid, monosaccharide, nucleic acid, fat or oil, phosphate group, glycerol, disaccharide, dipeptide, phospholipid, polysaccharides 17. Indicators (Benedict’s, Iodine, Biuret, Sudan III) 18. Enzyme structure, function, specificity 19. Induced fit model of enzyme function 20. Catalytic cycles (enzyme-substrate complex) 21. Factors affecting enzyme function (temperature, pH, cofactors & coenzymes, inhibitors) 22. Allosteric Regulation 23. Control of metabolism (feedback inhibition and structural order) 24. Prokaryotes vs. Eukaryotes 25. Animal Cell vs. Plant Cell 26. Organelles 27. Cell Membranes: structure and function 28. Transport Across the Cell Membrane: diffusion, osmosis, facilitated diffusion, active transport, bulk transport Unit #2 – Metabolic Processes (~ 22%) 1. Metabolism: anabolic and catabolic reactions 2. Potential vs. Kinetic Energy 3. Laws of Thermodynamics 4. Free Energy – endergonic and exergonic reactions 5. ATP 6. Understand how energy flows. 7. Know the difference between an oxidative and reductive reaction. 8. What are NAD+ and FAD? 9. What is the formula for cellular respiration? 10. Be able to sketch and label a diagram of a simple mitochondria. 11. What are the four major steps in cellular respiration? Where does each reaction occur? What are the reactants and products of each reaction? 12. Be able to distinguish between the two types of ATP production: substratelevel phosphorylation and chemiosmotic phosphorylation. 1 13. What is the net amount of energy produced for one molecule of glucose through respiration? 14. Be able to compare aerobic and anaerobic respiration. 15. What is the formula for photosynthesis? 16. Be able to draw and label a diagram of a chloroplast. 17. What is the electromagnetic spectrum? What portion of it is relevant for the study of photosynthesis? 18. What are pigments? What is special about chlorophyll’s structure? Name some secondary pigments. 19. What are the main stages of photosynthesis? 20. What are the reactants and products of the ‘LIGHT’ reactions? 21. Be able to follow the path of electrons through cyclic and non-cyclic electron pathways. 22. Be able to follow the path of CO2 through the Calvin cycle. 23. C3 is a common metabolic pathway, what are other pathways that can be used by plants? 24. What is photorespiration? 25. Understand the factors that influence photosynthesis 26. Be able to compare and contrast photosynthesis and cellular respiration. Unit #3: Homeostasis (~ 22%) 1. Introduction of Homeostasis 2. Feedback Systems (positive and negative feedback systems) 3. Maintaining Human Body Temperature 4. Stimulus, Receptor, Integrator/Regulator, Effector and Response 5. The Urinary System (know organs and structures involved) 6. Pathway of Human Urinary System 7. Kidney: Structure and Function 8. The Nephron: Structure and Function 9. Formation of Urine (Filtration, Re-absorption and Secretion) 10. Urine Output (ADH, Blood pH and buffers) 11. Kidney Diseases and Urinalysis 12. The Structure of the Nervous System (CNS and PNS – divisions) 13. Types of Nerve Cells (glial and neurons) 14. Sensory, Motor and Interneurons 15. Structure of a Neuron 16. Reflex Arc (5 essential components) 17. Resting Potential of a Neuron 18. Action Potential (initiation, refractory period, threshold level, all or none response, depolarization, repolarization) 19. How an action potential moves along the axon of a neuron 20. Synaptic Transmission (synaptic cleft, neurotransmitters, etc…) 21. The Brain: Structure and Function 22. The Spinal Cord 23 .Peripheral Nervous System (somatic, autonomic, sympathetic, parasympathetic) 24. Endocrine vs. Exocrine Glands 25. Glands and Hormones of the Endocrine System - definition of hormone - hormone action on target cells - steroid vs. water-soluble hormones - negative feedback loops and hormonal regulation 26. Effect of Endocrine Hormones - hypothalamus, pituitary gland, thyroid, parathyroid, pancreas, adrenal glands, testes and ovaries - related disorders 27. Male and Female Reproductive Systems and Hormones 28.The Menstrual Cycle Unit #4: Molecular Genetics (~ 22%) 1. Scientists and Experiments - Griffith, Avery and Colleagues, Hershey-Chase, Chargaff, Watson and Crick, Franklin 2. DNA Structure 3. The Structure and Organization of Genetic Material in Prokaryotes vs. Eukaryotes 4. DNA Replication – Semi-conservative theory (Meselson-Stahl Experiment) - initiation, elongation, termination, proofreading and correction 5. Comparing DNA Replication in Prokaryotes vs. Eukaryotes 6. One Gene-One Polypeptide Hypothesis - Garrod, Beadle and Tatum 7. The Genetic Code and Gene Expression: An Overview - central dogma, types of RNA, DNA vs. RNA, the genetic code 8. Transcription: From DNA to RNA - initiation, elongation, termination, posttranscriptional modification of mRNA 9. Translation: From RNA to protein - tRNA, ribosomes, initiation, elongation, termination 10. Mutations and Mutagens - types (point vs. chromosome), causes, repair mechanisms 11. Regulation of Gene Expression - the operon model in prokaryotes - lac and trp operon - gene regulation in eukaryotes 12. Recombinant DNA 13. Restriction Enzymes 14. Plasmids 15. Bacterial Transformation 17. Gel Electrophoresis 18. The Polymerase Chain Reaction 19. Restriction Fragment Length Polymorphism (RFLP) Analysis 20. Production and Regulation of Genetically Engineered Organisms Unit #5: Population Ecology (~ 12%) 1. Characteristics of populations 2. Measuring Populations (quadrat and mark-recapture methods) 3. Dispersion Patters (clumped, uniform, random) 4. Life Histories and Populations (survivorship curves) 5. Changes in Population Size (growth rate, exponential & logistic growth) 6. Factors that Regulate Natural Populations 7. Growth Model Calculations