Automated Cycle Sequencing - Albert Einstein College of Medicine

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SAMPLE INFORMATION FORM
DNA SEQUENCING FACILITY
Albert Einstein College of Medicine
713 Ullmann, x2657
Facility e-mail:[email protected]
David Reynolds: [email protected]
PROVIDE 2.0 l OF DNA TEMPLATE DISSOLVED IN H2O OR EB
BUFFER AND 3.2 l OF PRIMER DISSOLVED IN H2O
Investigator information
Principal Investigator
Department
MIXED TOGETHER FOR A TOTAL VOLUME OF 5.2 l
IN A 0.2 ML MICROAMP TUBE
AT THE CONCENTRATIONS LISTED BELOW
Date Submitted
Contact Person
Telephone Number
E-Mail Address
Rm/Bldg Address
Grant #
9 - 526 -
- 431
PURIFICATION: DNA for the automated sequencing facility
should be prepared with a QIAGEN spin column and dissolved in
H2O or EB buffer (do NOT dissolve in TE buffer). This will assure
the most consistent and highest quality sequence data.
QUANTIFICATION: You must quantify your DNA by running 1ul
on a gel adjacent to a DNA concentration standard. In addition, we
recommend you take an OD reading of your sample.
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The purity and concentration of both the template and the
primer is critical for automated sequencing.
Template Type
Size (kb)
DNA
TEMPLATE:
Plasmid (double-stranded DNA)
Cosmid DNA or  DNA
BAC DNA
PCR product
PRIMER:
For Plasmid or PCR product
For cosmid DNA or  DNA
For BAC DNA
Concentration
Volume
200 ng/l
600 ng/l
1200 ng/l
15 - 90 ng/l
2.0 l
2.0 l
2.0 l
2.0 l
1 pmol/l
5 pmol/l
15 pmol/l
3.2 l
3.2 l
3.2 l
SEQUENCE RESULTS: The facility provides a printout of
the sequence chromatogram and an e-mail text file.
Special Request: ___________________________________
Primer Name
Primer Tm
Notes
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