Mystery Gene Animation Worksheet Name: After reviewing the

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Mystery Gene Animation Worksheet
Name: ________________________________________________
After reviewing the online animation about DNA sequencing technology
(http://www.dnalc.org/ddnalc/resources/cycseq.html), answer the following questions.
1) List FIVE key components you need in order to sequence a piece of DNA.
2) To start the DNA sequencing reaction, the mixture is heated. Why?
3) Next, the mixture is cooled. Why?
4) Then, the mixture is slightly heated again. Why?
5) What happens each time a dideoxynucleotide is incorporated into the new DNA
strand?
6) Why are “dideoxynucleotides” added to the DNA sequencing reaction mix?
7) How are the DNA fragments separated on the polyacrylamide gel?
8) What do the colors in the electropherogram represent?
BLUE =
RED =
GREEN =
BLACK =
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