Cell Biology (BIO 320)
Chapter 13 – Intracellular vesicular traffic
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Introduction (Page 749)
II.
Molecular mechanisms of vesicular traffic (pages 750-765)
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Transport from the ER through the Golgi apparatus (pages 766-779)
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IV.
Vesicular transport – budding and fusion of vesicles (figure 13-2)
Intracellular compartments involved in the biosynthetic-secretory and endocytic
pathways (figure 13-3)
Various types of coated vesicles (figures 13-4 and 13-5)
Clathrin-coated vesicles and structure of clathrin coat (figures 13-6 and 13-7)
Assembly and disassembly of a clathrin coat (figure 13-8)
Other types of coats – example of retromer assembly (figure 13-9)
Phosphoinositides mark organelles and membrane domains (figures 13-10 and 13-11)
Role of cytoplasmic proteins in pinching-off and uncoating of coated vesicles (figure 1312)
Monomeric GTPases control coat assembly
ARF proteins responsible for COPI coat assembly and clathrin coat assembly at
Golgi membrane
Sar1 protein responsible for COPII coat assembly at the ER membrane (figure 1313)
Rab proteins guide vesicle targeting (figures 13-14 and 13-15)
SNAREs mediate membrane fusion (figures 13-16 and 13-17)
Interacting SNAREs are separated before they can function again (figure 13-18)
Viral fusion proteins and SNAREs may use similar fusion mechanisms (figure 13-19)
Proteins leave the ER in COPII-coated vesicles (figure 13-20)
Only properly folded and assembled proteins can leave the ER (figure 13-21)
Transport from the ER to the Golgi Apparatus
Homotypic membrane fusion (figure 13-22)
Vesicular tubular clusters (figure 13-23)
The retrieval pathway to the ER uses sorting signals (figure 13-24)
Many proteins are selectively retained in the compartments in which they function
The Golgi apparatus (figures 13-25 and 13-27)
Oligosaccharide processing and glycosylation events in the Golgi apparatus (figures 1328, 13-30 and 13-31)
Proteoglycans are assembled in the Golgi apparatus (figure 13-32)
What is the purpose of glycosylation?
Transport through the Golgi apparatus – vesicular transport or cisternal maturation
(figure 13-35)
Golgi matrix proteins
Transport from the Trans Golgi Network to lysosomes (pages 779-786)
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Lysosomes are the principal sites of intracellular digestion (figure 13-36)
Multiple pathways deliver materials to lysosomes (figure 13-42)
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Mannose 6-phosphate receptor recognizes lysosomal proteins in the TGN (figure 13-43)
The M6P receptor shuttles between specific membranes (figure 13-44)
A signal patch in the hydrolase provides recognition for M6P addition (figure 13-45)
Defects in the GlcNAc phosphotransferase cause a lysosomal storage disease in humans.
Endocytosis (pages 787-793)
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VI.
Specialized phagocytic cells can ingest large particles (figures13-46 and 13-47)
Pinocytic vesicles form from coated pits in the plasma membrane (figure13-48)
Not all pinocytic vesicles are clathrin-coated (figure 13-49)
Receptor-mediated endocytosis and LDL (figures 13-50, 13-51 and 13-53)
Possible fates for transmembrane receptor proteins that have been endocytosed (figure
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Exocytosis (pages 799-809)
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The constitutive and regulated secretory pathways (figure 13-63)
Protein sorting in the TGN (figure 13-64)
Secretory vesicles bud from the TGN (figures 13-65 and 13-66)
Proteolytic processing of proteins during the formation of secretory vesicles (figure 1367)
Secretory vesicles wait near the plasma membrane until signaled to release their contents.
Synaptic vesicles can form directly from endocytic vesicles (figures 13-73 and 13-74)