DETERMINATION OF SELECTED PSYCHOTROPIC DRUGS IN HUMAN SERUM BY LIQUID
CHROMATOGRAPHIC-MASS SPECTROMETRY.
Uřinovská R1., Brozmanová H.1, Šištík P.1, 2, Grundmann, M.1
1
Department of Clinical Pharmacology Faculty Medicine, University of Ostrava and University Hospital
Ostrava
2
Department of analytical chemictry, Faculty of science, Palacky University, Olomouc
Introduction: Psychiatric disorders such as depression and schizophrenia contribute significantly to
worldwide morbidity and mortality. Currently, hundreds of psychotropic drugs are available, with more or less
specific effects on symptoms of particular mental disorders. Despite advanced therapeutic and diagnostic
possibilities, still significant number of patients responds poorly to the treatment. The introduction of routine
therapeutic drug monitoring of certain psychotropic drugs could help to optimize and personalize
pharmacotherapy according to the needs of particular patient. Today, liquid chromatography and gas
chromatography are basic techniques for determination of psychotropic medication.
Method: A rapid and simple ultra performance liquid chromatography – tandem mass spectrometry
method was developed to simultaneously determine sertraline, N-desmethylsertraline, flouxetine, Ndesmethylsertraline, citalopram, desmethylcitalopram, didesmethylcitalopram, paroxetine, mirtazapine,
venlafaxine, N-desmethylvenlafaxine, O-desmethylvenlafaxine, clozapine, N-desmethyllozapine, olanzapine, Ndesmethylolanzapine, 2-hydroxyolanzapine, risperidone, 9-hydroxyrisperidone and trazodone. The preparation
of serum samples included precipitation protein and as internal standard was used alprenolol. Response of each
drug was compared both by electrospray in positive mode and atmospheric pressure chemical ionization.
Chromatographic separation was carried out on RP column BEH C18, using gradient mobile phases. The
detection was performed on a triple-quadrupole tandem mass spectrometry by multiple reaction monitoring
mode using electrospray in positive mode as ionization mode. Time of analysis was 5 min.
Results: Correlation coefficients of calibration curves were 0.995 – 1.0. Coefficients of variation were 4.2
-9.5% for intra-assay and 3.0-11.9% for inter-assay. Recoveries were 87.1 – 110% for intra-assay and 88.1 –
108.2% for inter-assay.
Conclusion: This method was fully validated and can be successfully applied for rutine analyses.