Βιογραφικό Σημείωμα Antonia V. Charchanti Born: February 24, 1969 Trikala, Greece Citizenship: Greek Office Address : Department of Anatomy-Histology-Embryology University of Ioannina School of Medicine 45110 Ioannina, Greece Tel. 26510-97832 FAX: 26510-97861 E-mail: acharcha@cc.uoi.gr Education 11/1994: Medical Degree University of Ioannina School of Medicine 12/1994-07/2001: Residency, Pathology Dept of Pathology, Univeristy of Ioannina School of Medicine 05/1998-05/1999: Residency, Cytology Dept of Pathology, Univeristy of Ioannina School of Medicine 03-04/1999: Research Fellow, Electron Microscopy Christie Hospital NHS Trust,Manchester, U.K Dr. Brian Eyden Head of Diagnostic Electron Microscopy Department of Histopathology Christie Hospital NHS Trust Manchester-U.K. (grant) 07/1999: Research Fellow, Electron Microscopy Christie Hospital NHS Trust,Manchester, U.K Dr. Brian Eyden Head of Diagnostic Electron Microscopy Department of Histopathology Christie Hospital NHS Trust Manchester-U.K. (grant) 01-03/2003: Research Fellow, Electron Microscopy Dept of Electron Microscopy, Research Center of «G Papanikolaou» at St. Savas Hospital, Athens 06/2002: Degree, Doctor of Science Dept of Pathology, Univeristy of Ioannina School of Medicine 2 Post-Doctoral Training 1. “Borderline lesions and tumors" Postgraduate Course, organized by the European School of Oncology 6-8 April 1995, Athens, Greece 2. ΄΄Stromal reaction in preneoplastic and early cancerous lesions΄΄ European Course of Cellular Pathology supported by the European Society of Pathology (ESP) and the European Cell Biology Organization (ECBO), in collaboration with the European School of Oncology (ESO), 9-14 May 1997, Metsovo, Ioannina / Greece * 3. Part I-General Pathology Postgraduate Course in theoretical and diagnostic histopathology organized by the departments of Pathology of the Democritus University of Thrace and the University of Ioannina / Greece in collaboration with the St.Bartholomew’s and the Royal London School of Medicine and Dentistry of the University of Sheffield Medical School, U.K. 10-14 September 1998, Alexandroupolis, Greece 4. ΄΄Lung Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 13-17 October 1998, Ioannina * 5. ΄΄Breast Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 11-15 May 1999, Ioannina * 6. ΄΄Prostate Pathology – Oncology Ioannina University Courses in Pathology (IUCP) 12-16 October 1999, Ioannina * 7. ΄΄Thyroid Pathology – Oncology΄΄ Ιoannina University Courses in Pathology (IUCP) 22-24 May 2000, Ioannina * 8. ΄΄Salivary glands Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 18-20 May 2000, Ioannina * 9. ΄΄Oesophagus-Stomach Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 15-17 May 2001, Ioannina * 10. ΄΄Duodenum-Biliary tract-Pancreas Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 17-19 May 2001, Ioannina * 11. ΄΄Small intestine Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 3 28-29 May 2002, Ioannina * 12. ΄΄Large intestine Pathology – Oncology΄΄ Ioannina University Courses in Pathology (IUCP) 30 May -01 June 2002, Ioannina * Professional Appointments 03/2003-9/2003: Consultant, Pathologist General Hospital – Medical Center Filiaton Thespotria 09/2003-2009: Lecturer Department of Anatomy-Histology-Embryology University of Ioannina School of Medicine 2009-today Assistant Professor Department of Anatomy-Histology-Embryology University of Ioannina School of Medicine Medical Societies and Associations 1. European Society of Pathology 2. Hellenic Anti-Cancer Society Research Grants 08/1999-10/1999: Collaborator ''Development of Pathology Training System» Work-program Νο 665 TEMETEN Towards a European medical and teleworking network, 06/2006-04/2009: Prinicipal Investigator Pro-herbs Work-Program Interreg IIIB Archimed (Ε.Ε. 63/2077) 2008-2009 Collaborator University Structural Support 2007-2008, Programs of Academic Excellence University of Ioannina “Neural cell death – examination of molecular mechanisms of apoptosis using tissue microarrays”. 251,200.00Ε 4 Publications 1. Ioachim E., Charchandi A., Stavropoulos N.E., Athanassiou E.and Agnantis N. J.. Expression of the cell adhesion molecule CD44 in transitional cell carcinoma of the urinary bladder. Journal of Tumor Marker Oncology 1997; 12 (4): 27-36, 2. Skopelitou Α., Mylonakis E,. Charchanti A, Kappas A.. Cellular Neurilemoma (Schwannoma) of the Descending Colon Mimicking Carcinoma. Dis Colon Rectum 1998; 41(9): 1193-96 3. .Eyden B, Yamazaki K., Menasce L.P, Charchanti A., Agnantis N. J. Basement-membranerelated peri-vascular matrices not organised as a basal lamina: distribution in malignant tumours and benign lesions J. Submicrosc. Cytol. Pathol. 2000; 32 (4), 515-523, 4. Ioachim E., Charchanti A., Stavropoulos N. E.,. Skopelitou A, Athanassiou E. D. and Agnantis N. J. Immunohistochemical expression of Retinoblastoma gene product (Rb), p53 protein, MDM2, c-erbB-2, HLA-DR and proliferation indices in human urinary bladder arcinoma Histol Histopathol 2000; 15: 721-727 5. Tatsioni A., Charchanti A, Kitsiou E, Ioannidis PA J. Appendicectomies in Albanians in Greece: outcomes in a highly mobile immigrant patient population BMC Health Services Research 2001 1:5 6. .Ioachim E,.Charchanti A,. Stavropoulos N, Athanassiou E, Michael M., and Agnantis N. Localization of metallothionein in urothelial carcinoma of the human urinary bladder: An immunohistochemical study including correlation with HLA-DR antigen p53, and proliferation indices. Anticancer Research 2001 May-Jun;21(3B):1757-61. 7. Stavropoulos N, Ioachim E, Charchanti A, Michael M., Kitsiou E., Mihailidis I., Hastazeris K. and Agnantis N.. Tumor Markers in Stage P1 Bladder Cancer Anticancer Research 21: 1495-1498 (2001) 8. Ioachim E., Charchanti A., Briasoulis E., Karavasilis V., Tsanou H., Arvanitis D. L., Agnantis N.J and Pavlidis N. Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: Their prognostic value and role in tumor invasion and progression. Eur J Cancer 2002 Dec; 38 (18): 2362-70 9. Ioachim E., Charchanti A., Stavropoulos N., Athanassiou E., Bafa M. and. Agnantis N. J. Expression of cathepsin D in urothelial carcinoma of the urinary bladder: An immunohistochemical study including correlations with extracellular matrix components, CD44, p53, Rb, c-erbB-2 and the proliferation indices. Anticancer Research 2002 Nov-Dec; 22 (6A): 3383-8 10. Kalef-Ezra J., Michalis L.K., Malamou-Mitsi V., Tsekeris P., Katsouras C., Boziari A., Toumpoulis I., Bozios G., Charchanti A., Sideris D.A.. External beam irradiation in angioplasted arteries of hypercholesterolemic rabbits. The dose and time effect. Cardiovasc Radiat Med. 2002 Jan-Mar; 3 (1): 20-25 11. Ioachim E., Tsanou H.,. Briasoulis E, Batsis Ch., Karavasilis V., Charchanti A., Pavlidis N.and Agnantis N.J.. Clinicopathological study of the expression of hsp27 and pS2, Cathepsin D and metallothionein in primary invasive breast cancer. Breast 2003 Apr; 12(2):111-9. 12. Ioachim E., Charchanti A., Charalabopoulos K., Tsanou H., Briasoulis E., Karavasilis V., Pavlidis N.,. Agnantis N J. The prognostic evaluation of tumor angiogenesis in invasive 5 breast carcinoma Electronic Journal of Pathology and Histology Volume 8, Numbers 14/2002/2003 \ 13. Charchanti A., Ioachim E., Eyden B., and Agnantis N. J. Ultrastructural and immunohistochemical observations on amorphous matrices related to basal lamina in breast cancer: preliminary data. Electronic Journal of Pathology and Histology Volume 8, Numbers 1-4/2002/2003 14. Ioachim E, Charchanti A, Briasoulis E, Karavasillis V., Batsis C.,, Pavlidis N and Agnantis NJ. The prognostic value of vascular endothelial growth factor (VEGF) in invasive breast cancer: Correlation with microvessel density (MVD), ER, PgR, p53 and proliferative associated indices (Ki-67, PCNA) 2002;. Electronic Journal of Pathology and Histology 8 (4), pp. 24071-2400710 15. Tsanou E, Ioachim E, Briasoulis E, Damala K, Charchanti A, Karavasilis V, Pavlidis N, Agnantis NJ. Immunohistochemical expression of superoxide dismutase (MnSOD) antioxidant enzyme in invasive breast carcinoma. Histol Histopathol. 2004 Jul;19(3):807-13. 16. Tsanou E, Ioachim E, Briasoulis E, Charchanti A, Damala K, Karavasilis V, Pavlidis N, Agnantis NJ. Clinicopathological study of the expression of syndecan-1 in invasive breast carcinomas. Correlation with extracellular matrix components. J Exp Clin Cancer Res. 2004 Dec;23(4):641-50. 17. Kitsoulis P, Vlychou M, Papoudou-Bai A, Karatzias G, Charchanti A, Agnantis NJ, Bai M. Primary lymphomas of bone. Anticancer Res. 2006 Jan-Feb;26(1A):325-37. Review. 18. Polyzos PT, Arvanitis LD, Charchanti A, Galani V, Havaki S, Kallioras VA, Nakou M, Faros EG, Marinos E, Sgantzos MN, Kittas C. Decidualized and pre-decidualized normal endometrial stromal cells produce more O-linked N-acetylglucosamine containing epitope H than non-decidualized normal endometrial stromal cells. Histol Histopathol. 2006 Nov;21(11):1193-8. 19. Sgantzos MN, Galani V, Arvanitis LD, Charchanti A, Psathas P, Nakou M, Havaki S, Kallioras V, Marinos E, Vamvakopoulos NC, Kittas C.Expression of the O-linked Nacetylglucosamine containing epitope H in normal myometrium and uterine smooth muscle cell tumors. Pathol Res Pract. 2007;203(1):31-7. Epub 2006 Nov 28. 20. Ananiadou OG, Bibou K, Drossos GE, Charchanti A, Bai M, Haj-Yahia S, Anagnostopoulos CE, Johnson EO. Effect of profound hypothermia during circulatory arrest on neurologic injury and apoptotic repressor protein Bcl-2 expression in an acute porcine model.J Thorac Cardiovasc Surg. 2007 Apr;133(4):919-26. Epub 2007 Feb 22 21. Kitsoulis P, Charchanti A, Paraskevas G, Marini A, Karatzias G. Adamantinoma. Acta Orthop Belg. 2007 Aug;73(4):425-31. Review. 22. Johnson EO, Charchanti A, Soucacos PN. Nerve repair: experimental and clinical evaluation of neurotrophic factors in peripheral nerve regeneration. Injury. 2008 Sep;39 Suppl 3:S37-42. Epub 2008 Aug 23 23. Johnson EO, Charchandi A, Babis GC, Soucacos PN. Apoptosis in osteoarthritis: morphology, mechanisms, and potential means for therapeutic intervention. J Surg Orthop Adv. 2008 Fall;17(3):147-52. 24. Ananiadou OG, Bibou K, Drossos GE, Bai M, Haj-Yahia S, Charchardi A, Johnson EO. Hypothermia at 10 degrees C reduces neurologic injury after hypothermic circulatory arrest in the pig. J Card Surg. 2008 Jan-Feb;23(1):31-8. 25. , Charchanti A, Verillis P., Ioachim E., Ananiadou OG, Johnson EO. Electron Microscopy evidence of Apoptosis after hypothermic circulatory arrest in the pig. (Submitted) 6 Proceedings 1. Malamou-Mitsi V, Ioachim E.,. Kamina S,. Skopelitou A,. Charchanti A and Agnantis. N. J.Immunohistochemical study of cell proliferation and apoptosis markers in breast neoplasia. Anticancer Research, Vol. 17: 4012-4013, 1997 2. Ioachim E,. Charchanti A,. Stavropoulos N. E, and Agnantis. N. J. Immunohistochemical expression of extracellular matrix component and Cathepsin D: A correlation with p53, Rb, cerbB-2 and the proliferative activity in the urinary bladder cancer. Anticancer Research Vol. 18: (6) 4883-4884, 1998. 3. Skopelitou A., Kamina S,. Charchandi A, and. Agnantis N. J. Immunohistochemical expression of the Retinoblastoma Gene Product in 110 Urinary Bladder Transitional Cell Carcinomas: A reliable prognostic marker in patients’ clinical outcome. Journal of Pathology Supplement 1998; 40A 4. Goussia A., Kordela V., Sintou–Mantela E., Zioga K., Panelos I., Charchanti A., Dimou S.,. Agnantis N.J, Bai M.. Expression of cell cycle proteins in bladder carcinomas Virchows Arch 447 (2): 700, 2005 5. Charchanti A., Goussia A., Balasi E., Zioga C., Fountzilas G., Kanavaros P., Agnantis N., Bai M.. Immunohistochamical expression of syndecan-1 protein in gastric cancer. Virchows Arch 448 (5): 723, 2006. 6. Lampri E., Goussia A., Sintou-Mantela E., Balasi E., Charchanti A., Kamina S., Capizzelo A., Tsekeris P., Briasoulis E., Malamou-Mitsi V.,. Agnantis N.J, Bai M.. Microvessel density and expression of angiogenic proteins VEGF, VEGFR1 and VEGFR2 in gastric adenocarcinoma. Virchows Arch 451 (2): 321, 2007. Chapters 1. Charchanti A., Soucacos P.N., Johnson E.O., Primary Histological Features in Acute and Chronic Infections, Infections of the Hand & Upper Limb, 2007, PMP(Paschalidis Medical Publications Ltd.) 2. Plissiti M. E, Charchanti A., Krikoni O., and Fotiadis D.I., “Automated segmentation of cell nuclei in PAP smear images,” IEEE, itab 2006, 26-28 October, Ioannina 3. Plissiti M.E., Tripoliti E.E., Charchanti A., Krikoni O. and Fotiadis D.I., Automated Detection of Cell Nuclei in PAP Stained Cervical Smear Images using Fuzzy Clustering, eMBEC 2008, 23-27 November, FC&CC, Antwerp, Belgium Chapters in Post-Doctoral Course Texts 1. Charchanti A., “Anatomy and Histology of the Endometrium, Ovaries and Accessories” Ιoannina University Courses in Pathology (IUCP)., Second Series, Endometrium, Ovaries and Accessories Pathology-Oncology, May 27-28, 2004, Ioannina 2. Charchanti A., “The normal breast: Anatomy, Histology and Function”, Ιoannina University Courses in Pathology (IUCP)., Second Series, Breast Pathology-Oncology, November 2-4, 2005, Ioannina 3. Charchanti A.,“The normal prostate: Histology and Function” Ιoannina University Courses in Pathology (IUCP)., Second Series, Prostate, Pathology-Oncology, May 25-27, 2005, Ioannina 4. Charchanti A.,“The anatomy of the Pleura and Mediastinum” Ιoannina University Courses in Pathology (IUCP)., Second Series, Pleura & Mediastinum PathologyOncology 4-5 June, 2009, Ioannina 7 Abstracts (International Meetings) 1. E, Kamina S, Charchanti A, Athanassiadou S and Agnantis N. J. Immunohistochemical glycoprotein CD44 expression in human breast cancer in correlation with the Basement Membrane antigens, Fibronectin, Cathepsin D, P53, C-erbB-2, proliferating indices, steroid receptor content as well as with the other conventional clinicopathological parameters in breast cancer. Euro cell Path - European School of Oncology Metsovo, Ioannina Greece 914 May 1997 (awarded) 2. Ioachim E, Kamina S, Athanassiadou S, Charchanti A and Agnantis N. J. Immunohistochemical expression of type IV Collagen, Laminin, Fibronectin and Cathepsin D: Correlation of proliferative activity, steroid receptors content and its clinicopathological significance in breast cancer. Euro cell Path - European School of Oncology Metsovo, Ioannina Greece 9-14 May 1997 3. V. Malamou-Mitsi, E. Ioachim, S. Kamina, A. Skopelitou, A Charchanti and N J. Agnantis. Immunohistochemical study of cell proliferation and apoptosis markers in breast neoplasia. 6th Hellenic Congress on Senology Alexandroupolis Greece, 12-16 June 1997 4. V. Malamou-Mitsi, E. Ioachim, S. Kamina, A. Skopelitou, A Charchanti and N J. Agnantis. Immunohistochemical study of cell proliferation and apoptosis markers in breast neoplasia. Ιinternational Conference Organized and Supported by the International Institute of Anticancer Research. Apoptosis meeting Athens, Greece 2-5 October 1977. 5. E. Ioachim, A. Charchandi, N.E. Stavropoulos, A. Christodoulou and N. J. Agnantis. Immunohistochemical expression of extracellular matrix component and Kathepsin-D:a correlation with P53, Rb, C-ERBB-2 and the proliferative activity in the urinary bladder cancer. XXVth Balkan Medical Week Ioannina 1-4 July 1998, Greece. 6. E. Ioachim, A. Charchandi, N. E. Stavropoulos and N. J. Agnantis Localization of metallothionein in human urinary bladder carcinoma: An immunohistochemical study including correlation with HLA-DR antigen, p53, Rb and proliferating indices. XXVth Balkan Medical Week Ioannina 1-4 July 1998, Greece. (Η εργασία αυτή βραβεύτηκε από την επιστημονική επιτροπή του συνεδρίου) 7. E. Ioachim, A. Charchandi, N. E. Stavropoulos, A. Christodoulou and N. J. Agnantis. Immunohistochemical expession of extracellular matrix component and CathepsinD: a correlation with P53, Rb, C-ERBB-2 and the proliferative activity in the urinary bladder cancer. 6th International Conference of Anticancer Research Halkidiki 21-25 October 1998, Greece. 8. A.S.Skopelitou, S.Kamina, A. Charchanti, N.J.Agnantis. Immunohistochemical expression of the Retinoblastoma gene product in 110 Urinary Bladder Transitional Cell Carcinomas: A reliable prognostic marker in patients’ clinical outcome. Pathological Society of Great Britain and Ireland, 176th Meeting, 7-9 January 1998, Synopses of papers, pp 40A 9. E. Ioachim, A. Charchanti, N.E. Stavropoulos, A. Skopelitou, E. Athanassiou and N. J. Agnantis. Ιmmunohistochemical expression of Retinoblastoma gene product (RB), p53 protein, MDM2, C-ERBB-2, HLA-DR and proliferation indices in human urinary bladder carcinoma. Pathological Society of Great Britain and Ireland Dundee, Summer Meeting 7th –9th July 1999 10. A.Charchanti , E. Ioachim, B. Eyden and N.J. Agnantis Ultrastructural and immunohistochemical observations on amorphous matrices related to basal lamina in 8 breast cancer. Euroworkshop I on Interdisciplinary Perspectives of Diagnostic Pathology, Cell Biology, and Morphometry Heidelberg, December 8-10, 2000 11. Α. Charchanti, E. Ioachim, N.J. Agnantis Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: The prognostic value and their role in tumor expansion and progression Euroworkshop II on Interdisciplinary Perspectives of Diagnostic Pathology, Cell Biology, and Morphometry Bruxelles, October 5-7, 2001 12. Charchanti A, Ioachim E, Briasoulis E and NJ Agnantis. Angiogenesis in invasive breast carcinoma: Association with extracellular matrix components and proteolytic enzymes. Euroworkshop II onInterdisciplinary Perspectives of Diagnostic Pathology, Cell Biology, and Morphometry Brussels, October 5-7, 2001 13. Ioachim E, Charchanti A, Briasoulis E, Karavasilis V, Τsanou H and N.J. Agnantis Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: The prognostic value And their role in tumor expansion and progression. HSBCR 5th INTERNATIONAL CONGRESS ‘where we stand with breast cancer research’ Athens, 1-2 November, 2001 14. Α. Charchanti, E Ioachim, Eyden B, and N.J. Agnantis Ultrastructural and immunohistochemical observations on amorphous matrices related to basal lamina in breast cancer. HSBCR 5th INTERNATIONAL CONGRESS ‘where we stand with breast cancer research’ Athens, 1-2 November, 2001 15. Charchanti A, Ioachim E, Briasoulis E and NJ Agnantis. Angiogenesis in invasive breast carcinoma: Association with extracellular matrix components and proteolytic enzymes. HSBCR 5th INTERNATIONAL CONGRESS ‘where we stand with breast cancer research’ Athens, 1-2 November, 2001 16. A. Charchanti, E. Ioachim and N.J. Agnantis Clinical significance of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in breast cancer. Euroworkshop III on Interdisciplinary Perspectives of Diagnostic Pathology, Cell Biology, and Morphometry Madrid, December 6-8, 2002 17. Charchanti A., Ioachim E and Agnantis NJ. Clinical significance of vascular endothelial Growth Factor (VEGF) and Microvessel Density (MVD) in Breast Cancer2.2nd International Mediterranean Course in Mastοlogy, September 25-26, 2003, Ioannina (Η εργασία αυτή βραβεύτηκε από την επιστημονική επιτροπή του συνεδρίου) 18. Goussia A., Kordela V., Sintou–Mantela E., Zioga K., Panelos I., Charchanti A., Dimou S.,. Agnantis N.J, Bai M.. Expression of cell cycle proteins in bladder carcinomas (Περ. αρ. P375).20th European Congress of Pathology September 3-8, Paris, France 19. Charchanti A., Goussia A., Balasi E., Zioga C., Fountzilas G., Kanavaros P., Agnantis N., Bai M. .Immunohistochemical expression of syndecan-1 protein in gastric cancer (Περ. αρ. P207).2nd Inter-Congress of the European Society of Pathology May 24-27, Ioannina 20. Bibou K, Ananiadou OG, Charchanti A, Drossos GE, Bai M, Johnson EO. Profound hypothermic circulatory arrest is associated with increased Bcl-2 immunoreactivity in selectively vulnerable brain regions, 2nd Inter-Congress of the European Society of Pathology May 24-27, Ioannina 21. Doukas M, Papoudou-Bai A, Lampri E, Kamina S., Charchanti A., Rontogianni D., Agnantis NJ., P. Kanavaros, , M. Bai. Apoptosis Profile of normal human thymus. 2nd Inter-Congress of the European Society of Pathology May 24-27, Ioannina 9 22. Plissiti M. E, Charchanti A., Krikoni O., and Fotiadis D.I., “Automated segmentation of cell nuclei in PAP smear images,” in proc. IEEE International Special Topic Conference on Information Technology in Biomedicine, Greece, Oct. 26-28, 2006. 23. Lampri E., Goussia A., Sintou-Mantela E., Charchanti A., Capizzelo A., Tsekeris P., Briasoulis E., Malamou-Mitsi V., Agnantis N.J., Bai M.. Microvessel density and expression of angiogenic proteins VEGF, VEGFR1 and VEGFR2 in gastric adenocarcinoma (Περ. αρ. P28)1st Hellenic-Jordanian Congress of Pathology April 29-30, Crete 24. Lampri E., Goussia A., Sintou-Mantela E., Balasi E., Charchanti A., Kamina S., Capizzelo A., Tsekeris P., Briasoulis E., Malamou-Mitsi V., Agnantis N.J., Bai M.. Microvessel density and expression of angiogenic proteins VEGF, VEGFR1 and VEGFR2 in gastric adenocarcinoma (Περ. αρ. PP2-103). 21st European Congress of Pathology September 813, Instabul, Turkey 25. Koutsogiannidis H.,. Ananiadou O.G,. Bibou A.N, Charchanti A., Bai M., Drossos G.E., Johnson E.O., Neuroprotection with profound hypothermia to 10 C during hypothermic circulatory arrest in an acute porcine model measures of apoptosis in the primary cortex. 14th World Congress on Heart Disease, Toronto, On, Canada, July 26-29, 2008 26. Plissiti M.E., Tripoliti E.E., Charchanti A., Krikoni O. and Fotiadis D.I., Automated Detection of Cell Nuclei in PAP Stained Cervical Smear Images using Fuzzy4th European Congress for Medical and Biomedical Engineering, 23 – 27 November 2008, Antwerp, The Netherlands. 10