AP Biology Quarter 2: Weeks 10-12
October 24 – November 10, 2011
DATE
IN CLASS
HOMEWORK
Tuesday
(2,3; 95 min.)
10/25/11
Patterns of Inheritance: X2 analysis
Thursday
(2,3; 95 min.)
10/27/11
Assessment: Patterns on Inheritance
Molecular Genetics
Monday
(2,3; 95 min.)
10/31/11
Molecular Structure: DNA Structure and Replication 1. Ch 10.6-16 SG
DUE: W 11/2
* How does the structure of DNA allow for its precise 2. Prearrange/Permission Slip
replication?
DUE: F 11/4
Wednesday
(2,3; 80 min.)
11/2/11
Molecular Genetics: Central Dogma
Protein synthesis: prokaryotes vs. eukaryotes
* How does the reproduction of organisms result in
genetic diversity? Of what value is genetic
diversity? How does statistical analysis aid our
understanding of genetics?
* Demonstration of Understanding
* How does the molecular structure of DNA allow for
its precise duplication?
* How does the molecular structure of DNA allow it
to code for proteins?
Friday
(2,3; 95 min.)
11/4/11
Gene Regulation: prokaryotes vs. eukaryotes
Tuesday
(2,3; 95 min)
11/8/11
Introduction to Biotechnology techniques
Lab 6a: Bacterial Transformation
C. Gay 10/23/11
* How do organisms regulate the production of
proteins? Why is gene regulation important for
survival?
* How does the structure and function allow us to
manipulate molecular processes? How can DNA be
transferred from one organism to another? What
are the risks of doing this?
1. Epigenetics Webquest
DUE: Th 10/27
3. Genetics Assessment
DUE: Th 10/27
1. Ch 10.1-5 SG or Notecard
DUE: M 10/31
2. DNA to Protein Webquest
DUE: M 10/31
1. Ch 11 SG
DUE: F 11/4
2. Prearrange/Permission Slip
DUE: F 11/4
1. AP Lab 6a Prelab (Green+Blue)
DUE: T 11/8
2. Ch 12.1-10 SG
DUE: T 11/8
3. All Biotech Postlabs
DUE: F 11/18
1. AP Lab 6b Prelab (Gold + Pink)
DUE: M 11/14
2. Ch 12.11-21
DUE: M 11/14
3. All Biotech Postlabs
DUE: F 11/18
SSHS AP Biology
Knowledge:
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Structure of Genetic Material
o Experiments of Griffith, Hershey and Chase
o DNA structure: nucleotides, nitrogenous bases, purines, pyrimidines, A, T, C, G, Watson, Crick, Franklin, double
helix, Chargoff’s rule
DNA Replication
o Helicase, DNA Polymerase, DNA Ligase, replication forks, replication bubbles, 3’-5’, Okazaki fragments,
Messelson and Stahl, semi-conservative replication
Protein Synthesis
o Beadle and Tatum: one gene, one polypeptide hypothesis
o Triplet code, codons, redundancy, non-ambiguous, start and stop codons
o Transcription: promoter, RNA polymerase, terminator, initiation, elongation, termination
o MRNA processing: polyA cap and tail, removal of introns, splicing exons, RNA as an enzyme
o Transfer RNA, anticodon, amino acid attachment site
o Ribosomes: large and small subunits, P site, A site
o Translation: initiation and ribosomal subunit binding, elongation (codon recognition, peptide bond formation,
translocation), termination
o Mutations: insertion and deletion (frame shift), mutagenesis
Viruses
o Lytic vs lysogenic cycles, Prophage
o Viral structure
o Plant and animal viruses, retroviruses, reverse transcriptase
Gene Regulation in Prokaryotes
o Operon: regulatory genes, promoter, operator, functional genes, repressor
o Types of operons: repressor (lac and tryp), activators
Cellular Differentiation and Cloning in Eukaryotes
o Cellular differentiation
o Cloning and regeneration
o Nuclear transplantation
o Embryonic stem cells vs. adult stem cells
Gene Regulation in Eukaryotes
o DNA Packaging: histones, nucleosomes, supercoils, centromere, kinetocore, telomeres
o X chromosome inactivation, Barr bodies
o Control of transcription: transcription factors, enhancers, silencers, alternative RNA splicing
o Control of translation: breakdown of mRNA, initiation of translation, protein activation, protein breakdown
Genetic Control of Embryonic Development
o Cascade of gene expression, cell-to-cell signaling, homeotic genes, regulatory protein gradients
o Signal-transduction pathway
o Homeobox sequences
Genetic Basis of Cancer
o Oncogenes, proto-oncogene, tumor suppressor genes, multiple hit hypothesis
o Environmental factors: carcinogens, mutagens, lifestyle choices
Bacteria as Tools for Manipulating DNA
o Bacterial transfer of DNA: transformation, transduction, conjugation
o F factor, plasmid, vector, plasmids
o Bacterial transformation: restriction enzymes, DNA ligase, recombinant DNA
o Gene cloning, genomic libraries
Tools of DNA Technology
o Reverse transcriptase and cDNA
o Nucleic acid probes
o DNA microarrays
o Gel Electrophoresis and RFLP analysis, blotting (southern, western, northern), autoradiography
o PCR
Human Genome
o Genetic (linkage) mapping, physical mapping, DNA sequencing, organisms sequenced
Bioethics
o DNA Fingerprinting
o Pharmacogenetics and recombinant organisms, therapeutic hormones, diagnosis and treatment of disease, vaccines
o GMO and agriculture
o Gene therapy
o Issues in biotechnology
Skills:
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Laboratory skills of biotechnology
Construction of restriction maps
Analysis and interpretation of RFLP analysis
C. Gay 10/23/11
SSHS AP Biology