PEER Teacher Requested Resource
Lesson Plan
Urinalysis
A Yellow Stain on an Otherwise Immaculate Lesson
Plan
Summary:
Ag students will get a crash course in urinalysis in this lesson. They will learn to do
everything from urine collection to looking at urine sediment under a microscope.
Keywords: urinalysis
Grade Level: 9-12
Agriculture TEK:
Veterinary Medical Applications 130.6
 (12) The student examines various aspects of clinical hematology. The student is
expected to:
o (A) describe laboratory tests and explain the importance of proper
laboratory procedures;
o (B) demonstrate the procedures used in collecting, handling, preparing,
and examining fecal, blood, and urine specimens;
o (D) explain sensitivity testing and how to read testing results; and
o (E) prepare microscope slides, preserve specimens, and perform several
of the most common laboratory tests.
Learning Objectives:
 Students will be able to look at slides of urine samples and be able to determine
what sediments/impurities are seen.
 Students will be able to look at the sediment seen and be able to determine the
medical cause of that sediment.
 Students will know the proper procedure for collecting urine samples.
 Students will be able to read a urinalysis stick test.
A product of the Partnership for Environmental Education and Rural Health at
College of Veterinary Medicine & Biomedical Sciences, Texas A&M University
Funding support from the National Center for Research Resources, National Institutes of Health
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Students will be able to read specific gravity on a refractometer.
Students will be able to perform a centrifugal sedimentation.
Time Required: 1-2 class periods
Materials:
 PowerPoint presentation
 Quiz PowerPoint
 Urinalysis sticks URS-10
 Refractometer
 Centrifuge
 Beaker/urine sample cup
 Distilled water
 Disposable tissues
 Gloves
 A refrigerator or cooler
 Test tubes
 Microscopes
 Pipettes
 Microscope slides
Reusable Activity Cost Per Group [in dollars]: ~400.72
Expendable Activity Cost Per Group [in dollars]: ~25.28
Background and Concepts for Teachers:
 Should be able to look at urine sample slides and identify and sediment seen and
know what the cause of that particular sediment.
 Should know how to read a urinalysis stick.
 Should know the proper procedure for collecting urine samples.
 Should know how to read the specific gravity on a refractometer.
 Should know how to perform a centrifugal sedimentation on a urine sample.
Vocabulary / Definitions:
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Urinalysis- the physical, chemical, and microscopic examination of urine. It
involves a number of tests to detect and measure various compounds that pass
through the urine.
Refractometer- A lab tool used for measuring the refractive index of a substance
like water or alcohol.
Specific Gravity- a lab test that measures the concentration of all chemical
particles in the urine.
Centrifugal Sedimentation- removing solids from liquids by causing particles to
settle through the liquid radially toward or away from the center of rotation by use
of a centrifuge.
A product of the Partnership for Environmental Education and Rural Health at
College of Veterinary Medicine & Biomedical Sciences, Texas A&M University
Funding support from the National Center for Research Resources, National Institutes of Health
Lesson Introduction / Motivation:
At this point in the lesson plan have the students begin practicing both free catch and
cage floor collection. Take this urine and store it in the cooler or refrigerator to be used
during the lesson.
Presentation/Explanation:
Show the students the PowerPoint presentation. Ask them questions about the pictures.
Have the students take notes and make drawings of the images shown.
Activity/Application:
For directions on the different activities, look at the activity plan.
Lesson Closure:
Show the students the Case Study Quiz PowerPoint. Have them write their answers on a
piece of paper to be turned in.
Assessment/Evaluation:
How well did they do on the quiz? This will show that they were paying attention to the
information provided and to the images shown. You can also grade their labs to see if
they grasped the concepts and procedures.
Safety Issues:
Be very careful when dealing with live animals. Wash hands thoroughly and wear gloves
as you will be handling urine. Be careful if using glass or if something breaks.
References:
 http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/150220.htm
 http://ahdc.vet.cornell.edu/clinpath/modules/ua-rout/rbcsed.htm
 http://irvingcrowley.com/cls/urin.htm
 http://ahdc.vet.cornell.edu/clinpath/modules/ua-rout/epithsed.htm
 http://www.vet.uga.edu/VPP/clerk/frost/index.php
 http://ahdc.vet.cornell.edu/clinpath/modules/ua-rout/castssed.htm
 http://www.enjoypath.com/cp/Chem/Urine-Morphology/Urine-morphology.htm
 http://meded.ucsd.edu/isp/1994/im-quiz/urine.htm
 http://renalfellow.blogspot.com/2009/06/urine-crystals-pattern-recognition.html
 http://ahdc.vet.cornell.edu/clinpath/modules/ua-rout/crystsed.htm
 http://www.proprofs.com/quiz-school/story.php?title=hematology-urinalysis
 http://www.2ndchance.info/kidney.htm/Ebook-And-Screensaver.html
 http://curiousanimals.net/pets/everything-about-persian-cats/
 http://www.acvim.org/websites/acvim/index.php?p=205
 http://www.advancesinhealth.com
 http://www.ehow.com/about_5332949_signs-symptoms-kidney-infectioncats.html
 http://www.kittyzom.com/
 http://www.revenuecurve.com/tag/best-girl-dog-names-2011
A product of the Partnership for Environmental Education and Rural Health at
College of Veterinary Medicine & Biomedical Sciences, Texas A&M University
Funding support from the National Center for Research Resources, National Institutes of Health
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http://www.nlm.nih.gov/medlineplus/ency/article/003579.htm
http://www.ehow.com/about_5408889_refractometer-definition.html
http://www.nlm.nih.gov/medlineplus/ency/article/003587.htm
http://encyclopedia2.thefreedictionary.com/centrifugal+sedimentation
Authors:
Undergraduate Fellow Name: Katie Clark
Please email us your comments on this lesson:
E-mail to ljohnson@cvm.tamu.edu
Please include the title of the lesson, whether you are a teacher or college faculty and
what grade you used it for.
A product of the Partnership for Environmental Education and Rural Health at
College of Veterinary Medicine & Biomedical Sciences, Texas A&M University
Funding support from the National Center for Research Resources, National Institutes of Health