DNA template
Sequencing primer
Ready Reaction Mix
2.5xsequencing buffer,ie
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr S. Minchin
Assessor
Rita Godfrey
Assessment Number*
1794
Date of Assessment
11/4/01
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Lab G10 and Genomics Lab
All occupants of lab G10 (Jo Field, Dong Ling Zeng, Rita Godfrey, Laura Tate)and
the Genomics lab.
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
DNA template
Sequencing primer
Ready Reaction Mix
2.5xsequencing buffer,ie
200mM Tris-HCl [pH 9.0],
5mM MgCl2
Sterile Water
75% Isopropanol
70% Ethanol
Hi-Di Formamide [99.5%
formamide, 0.11% EDTA,
0.39% water]
Big Dye Terminator sequencing
AMOUNT
per sample*
Attach copies of data sheet(s)
HAZARD
200500ng
3.2pmol
4ul
4ul
NSH under
GMAG rules
NSH
NSH
NSH
up to 20ul
80ul
NSH
FLAMMABLE
IRRITANT
250ul
10ul
FLAMMABLE
TOXIC
IRRITANT
5
RISK PHRASES
R11- Highly Flammable
R36-Irritating to the eyes
R67-Vapours may cause
drowsiness and dizziness
R11- Highly Flammable
R61-May cause harm to
the unborn child.Category
2 risk to reproduction
HAZDAT NO***
BIOSCIENCESNO***
127
84
261
R36/37/38-Irritating to
eyes, respiratory system
and skin.
INTENDED USE
Give brief details and attach protocol/instructions
Sequencing plasmid DNA samples using the Big Dye Terminator
kit and the 3700 DNA Analyser in the Genomics lab
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
There is a fire risk from using 75% Isopropanol and 70% Ethanol, and a possible risk of irritation to the
eyes from the isopropanol. Large quantities of isopropanol used on the open bench may cause drowsiness
or dizziness. The Hi-Di formamide solution poses a risk to pregnant women especially, and there is also a
risk to eyes, skin and the respiratory system from it.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The fire risk needs to be controlled. The risk from the Hi-Di Formamide is very slight due to the very small
volumes used, although the risk increases when aliquoting from the main stock into tubes.
8
CONTROL MEASURES
Additional to Good Chemical Practice
All sources of ignition should be removed when working with isopropanol or ethanol. Large volumes of
isopropanol should not be left out on the open bench next to the worker for more than a few minutes.
Tops should be replaced on bottles and tubes as soon as possible. Open bottles and tubes containing
Hi-Di Formamide should not be used in large quantities in the viscinity of pregnant women. Pregnant women
themselves who have to use this ought to handle it in a fume hood if there are a large number of
samples. The fume hood should be checked before use to make sure that it is working properly.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All waste isopropanol and ethanol should be collected in a waste non-halogenated solvent bottle which
when full should be taken down to the Stores for specialist disposal. All of the sequencing sample is
loaded onto the 3700 DNA Analyser and so is not returned to the user for disposal. Should there be any
Hi-Di formamide to dispose of, it should also be placed in the Waste Non-Halogenated Solvent bottle.
REVIEW
12
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
April 2006
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Isopropanol / Ethanol - Shut off all sources of ignition. Soak up small spills with the lab spill kit. Prevent material
from entering drains, and guard against static electricity. Transfer solvent to a suitable container.
Hi-Di Formamide – soak up with lab spill kit
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Personnel not involved in the clean up should stay out of the area. The affected area should be ventilated
in cases involving all the chemicals used in this assessment.
Ethanol or isopropanol – avoid build up of static electricity.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Thoroughly wash the contaminated area with lots of water in all cases.
CONTACT
Dr S. Minchin
0121 414 5438
PHONE 0121 414 5438
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.