QX1:
Sodium Acetate
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.J.E.Turnbull
Assessor
Laine Wallace
Assessment Number*
JETLW2
#2049
Date of Assessment
27/06/02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
5th Floor BiosciencesTower
Laine Wallace, Dr.J.E.Turnbull,K.Drummond,Z.Schofield,Dr.E.Yates, Dr,S Guimond,
Dr.Z.Huang, Dr.A.Powell, J.Henstock, Dr. T. Kinnunen
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Gel Extraction
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
QX1:
1.2ml
Harmful
R22 : Harmful if swallowed
Sodium Acetate
10ul
N/A
N/A
Isopropanol
400ul
Irritant/flamma
ble
R11 : Highly Flammable
R36 : irritating to the eyes
R67 : Vapours may cause
drowsiness and dizziness
PE
Tris
750ul
50ul
N/A
Irritant
N/A
R36/37/38: Irritating to eyes,
respiratory system and skin
5
HAZDAT NO***
BIOSCIENCESNO***
INTENDED USE**
Give brief details and attach protocol/instructions
QX1 – to dissolve gel and release DNA
Isopropanol – precipitation of DNA
Tris – Buffer.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
QX1 – Risk of splashes to unprotected skin and eyes
Isopropanol – Risk of inhalation and splashes to unprotected skin and eyes. Small risk of fire from stock due to
vapour travelling towards sources of ignition.
Tris - – Risk of splashes to unprotected skin and eyes
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Due to the nature of some of the chemicals listed in this assessment there is a small risk . But due to the small
amounts used and as long as measures laid out in section 8 are followed then the level of risk is acceptable. Non
of the chemicals used can be replaced with other “ less hazardous” reagents.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Wearing of a lab coat. Wearing of chemical resistant gloves.
Wearing of eye protection.
Small risk of fire with use of Stock Isopropanol – use in a fume hood, be aware of sources of ignition as vapour
may travel towards them.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
One to one initial training.
10
MONITORING
Performance of control measures,
N/A
Personal exposure
Health Surveillance
N/A
N/A
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All reagents at levels used within this protocol can be disposed of down the sink with copious amounts of water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
27/06/07
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Small spills of reagents can be mopped up with tissue and disposed of in the contaminated waste bag for
incineration.
Large spills can be contained with absorbant material, swept up into a contaminated waste bag for incineration.
TO PROTECT PERSONNEL
Evacuation, protection for personnel involved in clean-up, Special First Aid
If large amount of Isopropanol spilt then immediate area needs to be evacuated. All sources of ignition
extinguished, breathing apparatus and rubber boots worn for mop up with absorbant material.
Contact with reagents within this protocol require immediate washing in copious amounts of water. If discomfort
persists then contact a physician.
TO RENDER SITE OF EMERGENCY SAFE
Clean-up/decontamination
Contaminated areas should be cleansed with a dilute decon solution and dried off.
CONTACT
Dr.J.E.Turnbull
PHONE
47527
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.
Gel extraction
1. Excise gel slice- watch screen to see where scaple cutting
2. Weigh in ependorf and add 3 vols QX1 to 1 vol gel
Max gel is 400mg
3. Incubate at 50C for 10’ (until gel slice dissolved) – vortex every 2-3’
4. Check color of mixture yellow (CF QX1 buffer) (If orange or violet add 10ul 3M NaOAc)
5. Add 1 gel volume of isopropanol & mix-do not centrifuge
6. Place QIAQuick spin column in 2ml collection tube
7. Load sample (max 800ul) and spin 1’
8. Discard flow-through. If more than 800ul repeat 7
9. Wash 0.75ml PE, Spin 1’
10. Discard flow through and spin for 1’
11. Elute into labelled ependorf : 50ul or 30ul 10mM Tris (pH 7-8.5) to centre column, leave 1’, spin 1’
12. DNA to –20C