Supplementary Table 1. Tumour cell lines and culture media. All cells were maintained at 37°C and 5% CO
2
.
Cell line Media Cancer type Reference
U87MG
HT1080
A549
Capan 1
PC-3
LNCaP
MEM + 10% FCS, 2mM L-glutamine,
1% NEAA, 1% Sodium Pyruvate,
1.5g/L sodium bicarbonate
Brain
MEM + 10% FCS + 2mM L-glutamine
1% NEAA
Fibrosarcoma
DMEM + 10%FCS+2mM L-glutamine Lung carcinoma
RPMI + 10%FCS + 2mM L-glutamine
Ham F12 + 10% FCS + 2Mm Lglutamine
Pancreatic adenocarcinoma
Prostate carcinoma
RPMI + 10% FCS + 2mM L-glutamine Prostate carcinoma
DLD-1
COLO205 RPMI + 2mM L-glutamine 10mM
HEPES, 1mM Na Pyruvate, 1/100 glucose, 10% FCS
T98G
RPMI + 10% FCS + 2mM L-glutamine
+ 10mM HEPES + 1mm Na Pyruvate +
1/100 glucose
Hela
T47D
MEM + 10% FCS + 2mM Lglutamine+ 1% NEAA + 1% Sodium
Pyruvate
MEM + 10% FCS + 2mM L-glutamine
+ 1% NEAA
RPMI + 2mM L-glutamine + 1%
Colorectal adenocarcinoma
Colorectal adenocarcinoma
Glioblastoma
Cervix adenocarcinoma
Breast ductal
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U266
SHEP-1
1847
OVCA433
SKOV-3
JAMA2
Sodium Pyruvate + 10% FCS +
0.2U/ml Insulin
RPMI + 10% FCS + 2mM L-glutamine Blood myeloma
DMEM: EMEM 50:50 + 10% FCS +
2mM L-glutamine
SH-SY-5Y DMEM + 10% FCS + 2mM Lglutamine
IMR32 RPMI:DMEM 50:50 + 10% FCS +
2mM L-glutamine
SK-N-SH DMEM:EMEM 50:50 + 10% FCS +
2mM-glutamine
TR175 RPMI + 10%FCS +2mM glutamine
RPMI + 10%FCS +2mM glutamine
DMEM + 10% FCS + 2mM L-Glut
DMEM + 10% FCS + 2mM L-Glut
DMEM + 10% FCS + 2mM Lglutamine carcinoma
Neuroblastoma
(epitheloid subclone of SK-N-SH)
Neuroblastoma
Neuroblastoma
Neuroblastoma
Ovarian carcinoma
Ovarian carcinoma
Ovarian carcinoma
Ovarian adenocarcinoma
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SMYM-
PRGP
MMG1
MelJuso
SkMel5
RPMI + 10% FCS + 2mM L-glutamine
RPMI + 10% FCS +2mM L-glutamine
Primary Acral melanoma
Primary Acral melanoma
Murata H, Ashida A, Takata M, Yamaura M, Bastian BC, Saida
T (2007). Establishment of a novel melanoma cell line SMYM-
PRGP showing cytogenetic and biological characteristics of the radial growth phase of acral melanomas. Cancer Sci 98: 958-963.
A kind gift from Professor Akifumi Yamamoto (Saitama
Medical University, International Medical Center, Saitama,
Japan).
RPMI + 10% FCS + 2mM L-glutamine Malignant melanoma Johnson JP et al. Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin. Eur J Immunol 11: 825-31, 1981.
RPMI + 10% FCS + 2mM L-glutamine Malignant melanoma Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci.
USA 73: 3278-3282, 1976.