1 • Review questions for first Trends in Biotechnology classroom test. • The real test will be much shorter The fundamental unit of life is the ______. A) organism B) organ system C) organ D) tissue E) cell 3 Which type of microscope has a useful magnification limit of about 1,000X? A) light microscope B) transmission electron microscope C) scanning electron microscope D) scanning-probe microscope 4 ______ is the ability to distinguish between two adjacent points. A) Magnification B) Resolving power C) Ionization D) Emulsification E) Multiplication 5 Electrons have a shorter wavelength than visible light and therefore will have a lower resolving power. A) True B) False 8 Multicellular organisms are composed of many cells. A) True B) False 9 The cell theory states that all living things are made of atoms. A) True B) False 11 The ______ membrane separates the contents of the cell from the surrounding environment. A) plasma B) nuclear C) Golgi D) mitochondrial E) lysosomal 14 A threadlike material called chromatin is located within the ______. A) nucleus B) nucleolus C) mitochondria D) lysosome E) Golgi apparatus 19 At the time of cell division, rodlike structures called chromatin will form within the nucleus. A) True B) False MATCH the number with the letter. 1. Anton van Leeuwenhoek 2. Aristotle 3. Francesco Redi 4. John Needham 5. Louis Pasteur 6. Robert Brown 7. Robert Hooke A. Suggested that living organisms could develop from non-living matter. B. First person to suggest the rejection of idea of living organism developing form nonliving matter, but not all scientists accepted his results. C. Suggested that some microorganisms could develop from non-living material, if these microbes were small enough. D. Ended the idea of living material from non-living material by using better designed lab equipment, and gave rise to today’s cell theory. E. First person to observe cells under a microscope (invented microscope). F. Worked a lot at designing the early compound light microscopes and expanded cell theory; first person to observe living cells. G. First person to observe nucleus inside cell. III. MATCHING. 1. anaphase 2. cell cycle 3. cell division 4. DNA replication 5. interphase 6. metaphase 7. prophase 8. reproductive 9. somatic 10. telophase A. an intermediate stage of division when chromosomes pairs align along the equatorial plane of the cell spindle B. an intermediate stage of division when chromosome pairs move along spindle fibres to opposite ends of the cell C. complete duration or life-span of a cell D. consists of 6 phases, beginning with final interphase and ending with cytokinesis E. major portion of the life cycle of a cell F. occurs inside nucleus during final part of interphase in preparation for mitosis G. the early stage of division during which chromosomes condense and become visible H. the final stage of mitosis or meiosis during which the chromosomes of daughter cells are grouped in two new nuclei I. type of cell that reproduces asexually by meiosis, resulting in four new “halfcells” that contain half the number of chromosomes as the original cell J. type of cell that reproduces asexually by mitosis, resulting in two new cells that are exact duplicates of the original cell Use this diagram to label the numbered diagram. Match the numbers with the names. Chemicals of life 60 ______ are lipids containing phosphorus that are very important in cell membranes. A) Glycerol B) Fatty acids C) Triglycerides D) Steroids E) Phospholipids 63 Fats do not mix with water because they are polar molecules. A) True B) False 66 The polar portion of the phospholipid molecule (head) is soluble in water, whereas the two hydrocarbon chains (tails) are not. A) True B) False 68 Proteins are polymers of ______. A) amino acids B) nucleotides C) glycerol and fatty acid D) monosaccharides E) disaccharides 71 The bond that occurs between the acid group of one amino acid and the amino group of another amino acid is termed a (an) ______ bond. A) hydrogen B) weak C) peptide D) ionic E) covalent 73 Which of the following types of organic molecules have their monomers joined by a peptide bond? A) proteins B) lipids C) carbohydrates D) nucleic acids E) salts 82 What are the components of a nucleotide? A) glucose, cholesterol, triglyceride B) amino acid, fatty acid, phosphate C) phosphate, glucose, fatty acid D) phosphate, hexose sugar, nitrogen-containing base E) phosphate, pentose sugar, nitrogen-containing base 83 When nucleotide monomers join together, the ______ polymer is formed. A) protein B) carbohydrate C) lipid D) nucleic acid E) cholesterol http://www.questionpapers.net.in/question_papers/biology/biotechnology_1.html 3. The molecular formulae of deoxyribose sugar and ribose sugar are (A) C5 H10 O4 and C5H10O6 (B) C5 H10 O4 and C5H10O5 (C) C5 H10 O5 and C5H10O4 (D) C5 H10 O5 and C6H10O4 Answer: (B) 4. The nitrogen bases which pair with two hydrogen bonds are (A) Adenine and thymine (B) Adenine and Cytosine (C) Cytosine and guanine (D) Cytosine and adenine Answer: (A) 5. DNA differs from RNA in (A) Presence of deoxyribose sugar (B) Presence of thymine base (C) Property of replication (D) All the above Answer: (D) 6. DNA molecules makes a complete turn after every (A) 20 Å (B) 34 Å (B) 3.4 Å (D) 10 base pairs Answer: (D) 7. The distance between two successive nitrogenous base pairs is (A) 34 Å (B) 36 Å (C) 20 Å (D) 3.4 Å Answer: (D) 8. In nucleoside, nitrogen base is attached to pentose sugar at (A) Carbon – 1 of pentose sugar (B) Carbon – 2 of pentose sugar (C) Carbon – 4 of pentose sugar (D) Carbon – 5 of pentose sugar Answer: (A) 9. If the strand of DNA has 35 nucleotide how many phosphodiester bonds would exist (A) 34 (B) 35 (C) 24 (D) 70 1. __________ is the commercial use of living organisms or their components to improve animal and human health, agriculture, and the environment. a. Bioconversion b. c. d. Biotechnology DNA fingerprinting Bioremediation 2. __________ is the breakdown of organic compounds by cells or organisms in the absence of oxygen to generate ATP. a. Complementation b. Fermentation c. Electroporation d. Dehalogenation 3. __________ in the stomach worked with the natural bacteria in milk to cause the casein to precipitate and form curds, which were subsequently dried. a. Chromatids b. Enzymes c. Amino Acids d. Crustaceans 9. The renowned French Chemist __________ eventually established that yeast and other microbes are directly linked to fermentation. a. b. c. d. Louis Pasteur Anton van Leeuwenhoek Shen Nan CGIAR 13. __________ is added to fruit juice to improve taste. a. Lysine b. Methionine c. Cysteine d. Alanine 14. __________ is used in Japan to make bread a more complete protein. a. Cysteine b. Alanine c. Lysine d. Methionine 15. __________ prevents rancidity in various foods. a. Tryptophan b. Methionine c. Alanine d. Cysteine The following questions from http://highered.mcgrawhill.com/sites/0073031208/student_view0/chapter13/multiple_choice.html 1 Advantages to using the garden pea for Mendel's experiments included all listed below except A) true-breeding varieties were scarce. B) he could expect to observe segregation of traits among the offspring. C) they have relatively short generation time. D) sex organs of the pea are enclosed within the flower. 2 Pea plants can 3 A) self-pollinate, but are not self-compatible B) self-fertilize C) only cross-fertilize D) reproduce without pollination In Mendel's F2 generation, one out of four plants had white flowers because A) the trait is sex-linked B) both parents were heterozygous purple C) one parent was homozygous recessive D) both parents were heterozygous white 6 In the F2 generation, the 3:1 ratio is really a disguised A) 1:2:1 B) 2:1:1 C) 1:1:2 D) 4:0 E) 4:1 7 The ratio often referred to the Mendelian ratio is A) 1:3:3:1 B) 9:7 C) 1:3:1 D) 1:1 E) 3:1 8 When Mendel crossed pure-breeding purple and white flowered pea plants, the dominant to recessive ratio in the F1 generation was A) 3:1 B) 4:0 C) 4:1 D) 4:0 E) 9:3:3:1 9 On which of the following chromosomes are sex-linked traits carried? A) 13 B) 18 C) Y D) 15 E) X 11 In Mendel's F2 generation of the purple and white flower crossing, the dominant to recessive ratio was A) 1:3:1 B) 3:1 C) 1:1 D) 9:7 E) 9:3:3:1 15 A individual who has two of the same allele is said to be A) homozygous B) heteromologous C) homologous D) heterozygous E) diplozygous 16 Which of the following represents a dihybrid? A) WWSs B) WwSS C) WwSs D) WWss E) wwSs 17 Where two alternatives for a trait are tall and short, and tall is dominant, the genotype of a heterozygous individual would be expressed A) sS B) ss C) SS D) Ss E) tall 19 The appearance resulting from a given gene combination is referred to as the A) genotype B) phenotype C) phototype D) alleleotype E) stereotype 22 The two alternatives for a trait are red and white and red is dominant. However, white is the most common trait. What is the genotype of a homozygous dominant individual? A) RR B) rr C) WW D) ww E) red 24 A genetic disorder caused by a dominant allele is A) sickle-cell anemia B) PKU C) Tay-Sachs disease D) hemophilia (royal) E) Huntington's disease 27 In garden peas, height is determined by a single gene with tall being dominate to short. If two heterozygous plants are crossed, what proportion of the tall progeny will be homozygous dominant? A) 3/4 B) 2/3 C) 1/2 D) 1/3 E) 1/4 28 In the cross MMnn x mmNN, what proportion of the resulting F1 would be homozygous dominant for both genes? A) none B) 1/16 C) 3/16 D) 9/16 E) all 31 The model for the dihybrid cross of heterozygotes predicts a phenotypic ratio of 9:3:3:1. A) True B) False 35 A trait caused by a recessive autosomal allele will result in a pedigree that shows affected individuals having at least one affected parent. A) True B) False Morgan and Drosophila When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation included both red- and white-eyed flies. Remarkably, all the white-eyed flies were male. What was the explanation for this result? a) The gene involved is on the Y chromosome b) Other male-specific factors influence eye color in flies c) The gene involved is on an autosome d) The gene involved is on the X chromosome Thomas Hunt Morgan's choice of Drosophila melanogaster has been proven to be useful even today. Which of the following has/have continued to make it a most useful species? I. its four pairs of chromosomes II. a very large number of visible as well as biochemically mutant phenotypes III. easy and inexpensive maintenance IV. short generation time and large number of offspring A) I and IV only B) II and III only C) I, II, and III only D) II, III, and IV only E) I, II, III, IV, and V Pedigrees 17 Pedigrees for human genetic traits are important because: A) Humans tend to have small numbers of offspring. B) Matings between humans cannot be established by experimenters. C) individuals. Knowledge about family history can help determine probabilities for D) All of the above. E) None of the above. 18 Laurie met her husband Jim at a support group for families dealing with PKU (phenylketonurea), a recessive genetic disorder. Laurie and Jim both have one sister who is affected with PKU and no other family history of the disorder. They come to you as a genetic counselor to ask what their risk is of having an affected child. What do you tell them? A) They must both be heterozygotes so their risk of having an affected child is ¼. B) They might both be heterozygotes so they should get tested to see if they carry the disease allele or not. C) Since neither one of them is affected, they are not at risk of having an affected child. D) genotypes. You can't give them any firm answers because you don't know their 19 Which of the following statements about a pedigree would be most definitive in determining a recessive pattern of inheritance? A) Two affected individuals have three affected children. B) Two unaffected individuals have one affected and two unaffected children. C) There are four instances where at least one affected parent has an affected child. D) Two known heterozygotes have two affected and one unaffected child.