Section B
RODENT BREEDING COLONY
For rodents several variables to consider: 1) expected litter size for the strain; 2) Genotype of breeders will affect
usable offspring; add an extra female or two as a buffer, etc.
B1.
List species to be bred, indicate the number of breeders and provide estimates for the numbers of offspring
expected over a 3 year period.
# of Breeders
Species
Male
Female
Expected # of
Offspring
B2:
List the background stock/strain (e.g. C57BL/6, BALB/c, Sprague Dawley):
B3.
Describe the breeding/mating system that will be used.
Total # of
Animals
(Breeders +
offspring)
Number of
Animals Born, but
not used on this
AUF
(include in table on pg. 4)
B4.
a. Identify the tissue collection site for genotyping:
Distal Tail
Distal End of Toe
(tail tissue biopsy should be <
2mm; general anesthesia
required after 28 days of age)
(toe sampling should be
performed within 5-7 days of
age in mice)
Ear Pinna
(< 2mm punch)
Hair/Fur
(2 tufts; ~20 follicles)
Fecal Pellet
(~3-5 pellets)
Buccal Swabs / Saliva
(swab from inner cheeks at >3
days of age)
b. Will you be following the CAR Guideline for genotyping and tissue collection?
http://animalcare.msu.edu/guides (CAR4: Rodent Genotyping)
Yes
B5.
If no, please justify and describe your technique:
Will you be breeding genetically modified rodents that contain more than 50% of an exogenous eukaryotic virus?
Yes
B6.
No
No
Will you be breeding genetically modified rodents whose transgene is under the control of a functional gamma
retroviral (MLV, FIV, etc.) long terminal repeat.
Yes
June 2015
No
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