STEM32 Lesson Plan Template Teacher’s Name: Nancy Azcona School: MS 88 STEM32 PD Attended: STEM Training with Wildlife Conservation Society (WCS) Lesson Title Hatching Brine Shrimp Grade Level NYS Standards 6 to 8 5.1a Animals and plants have a great variety of body plans and internal structures that contribute to their ability to maintain a balanced condition. 5.1b An organism’s overall body plan and its environment determine the way that the organism carries out the life processes. Objective(s) Students will develop an understanding of how the growth and survival of an organism (brine shrimp) depends on physical conditions. Students will design an experiment to determine the optimum salinity of water needed to hatch brine shrimp. Materials and Websites Smart Start Mini-Lesson Application (Group Work) Magnifying glasses Containers in which to hatch brine shrimp (e.g., plastic 16-20 ounce water or soda bottles, from which the labels can be peeled so students can see inside the bottles, and the brine shrimp can get light) Spring water or aged tap water Rock or sea salt Growlite bulb—strong illumination is necessary for hatching; a standard growlite bulb, available in an aquarium supply store, should be sufficient Brine shrimp—these can be obtained from many pet or aquarium Provide students with a nature video or picture book to describe saltwater wetlands. Discuss the characteristics of saltwater wetlands. Animals that live in salt marshes, mangrove swamps, and tidal flats all have built-in adaptations to deal with saltwater and changing levels of salinity. For example, brine shrimp can survive in seawater and brackish water and can be found in salt lakes and brine ponds all over the world. However, brine shrimp have no defense against predation, so can best thrive in conditions in which their predators cannot, such as high salinity and temperature. 1. Place some brine shrimp eggs on a large piece of paper and have students view the eggs through a magnifying glass and describe what they see. What do they think they are seeing? 2. Tell students that they are looking at the eggs of an organism that lives in saltwater lakes and, like the animals that live in saltwater wetlands, is adapted to salty conditions. Explain that the eggs they are looking at can be hatched in saltwater, and that they will design an experiment to figure out just how salty the water should be in order to hatch the most eggs. 3. Ask students to brainstorm about ways in which they can determine the optimum level of salt in the water for hatching (i.e., the level of salt that allows the most eggs to hatch). 4. Next, have students work in groups to design an experiment to determine this optimum level of salinity for hatching. For example, students could set up several containers of water, putting freshwater in one and water that's increasingly salty in the others. 5. Have students answer the following questions: a) In this experiment, what is the dependent variable? (It is the number of eggs to hatch.) b) What is the independent variable? (Salinity.) c) How many different salinities will you test? d) What other variables will you keep constant? (Temperature, the number of eggs, etc.) e) How will you keep those variables constant? 6. Now have students carry out their experiments. Students should use only spring water or aged tap water in the mixtures, as brine shrimp are sensitive to poor water quality. In addition, instruct students to label all containers with the ratio of salt (rock salt) to water used. After the containers have been filled with salty water and labeled, have students add some eggs to each container and observe what happens (it will take a day or two for the eggs to hatch). Remember that strong illumination is necessary for hatching. Conclusion FYI: One tablespoon [15 ml] of salt mixed with one cup [240 ml] of water is usually a good mixture for hatching the brine shrimp eggs. Once students have completed their experiments, have them present their results to the class, highlighting what they found to be the optimum salinity for hatching (i.e., the salinity that allowed the most eggs to hatch). Students should display summaries of their experiments and conclusions on poster boards and accompany these with written reports. They should include tables or graphs to show the hatching rate for the various ratios of salt to water tested. Power Point presentations, Excel graphs, etc. can be used to enhance these reports. Extensions: In addition, have students provide written responses to the question: "How could this have been a better experiment?" You should expect students to note things such as better control of variables and more precise equipment, for example.