2010 Syllabus: PS 353 Plant Biotechnology, Genetics and Breeding (3) Genetic principles and techniques used in plant modification. Principles of molecular, and transmission quantitative genetics as applied to plant biotechnology. Prereq: Biology 111 and 112. Tuesdays and Thursdays 8:10-9:25; Ellington Plant Sciences 128 Neal Stewart, 4-6487, 320 Plant Biotechnology (nealstewart@utk.edu) Office hours 9:30-11:00 Tuesdays and Thursdays or by appointment. Guest Lecturers: Feng Chen, Dean Kopsell, David Mann, Vince Pantalone, Bob Trigiano, and Amy Yancey Text. Stewart, C.N., Jr. (Ed.) 2008. Plant Biotechnology and Genetics: Principles, Techniques and Applications, Wiley and Sons, Hoboken, New Jersey, 374 pp. Lecture powerpoints are at http://plantsciences.utk.edu/pbg/ Grading: 10 point scale (e.g., A-=90-91, A= 92-100), no curve. Each exam (2)= 32% (short answer format), Paper (1): 30%, class participation: 6%. The paper will focus on an application of plant biotechnology and/or breeding. It will take the form of a short scholarly article (1500 words) that is fully referenced Students can use Trends in Plant Science format. Two drafts of the paper will be submitted. The first is due March 28 and will be annotated and returned by April 6. The second version will be due on April 25. Each student will make a 10-15 minute presentation about his or her paper meant to teach. First and final drafts as well as the presentation will contribute to the paper grade. Lecture numbers 1 2 3, 4 5, 6 7 8, 9 10, 11 12, 13 14, 15 16, 17 18, 19 20, 21 22 23, 24 25, 26 8:00-10:00 am Date lecture lecturer Jan 14 Introduction Stewart Jan 19 Mendelian genetics & plant repro Trigiano Jan 21 & 26 Breeding Pantalone Jan 28 & Feb 2 Plant development & physiology Chen/Kopsell Feb 4 & Feb 9 Tissue culture Trigiano Feb 11 & 16 Molecular gene expression Stewart Feb 18 & 23 Recombinant DNA & vectors Stewart/Mann Feb 25 & Mar 2 Genes and traits of interest Stewart Mar 4 Midterm exam—through lectures 1-13 Mar 8-12 Spring break Mar 16 & Mar 18 Plant transformation Stewart Mar 23 & 25 Promoters and marker genes Stewart Mar 30 & Apr 1 Apr 6 & 8 Apr 13 Apr 15 Apr 20-22 Apr 27 & 29 May 6 Analysis of transgenic plants Regulations and biosafety Field testing and risks Intellectual property Controversies & future Student Presentations Comprehensive final exam reading Ch 1 Ch 2 Ch 3 Ch 4 Ch 5 Ch 6 Ch 7 Ch 8 Ch 10 Ch 9 Stewart Ch 11 Stewart Ch 12 Stewart Ch 13 Stewart/Yancey Ch 14 Stewart Ch 15, 16 In enrolling in this class student promises to abide by the UT Honor Statement “An essential feature of the University of Tennessee is a commitment to maintaining an atmosphere of intellectual integrity and academic honesty. As a student of the University, I pledge that I will neither knowingly give nor receive any inappropriate assistance in academic work, thus affirming my own personal commitment to honor and integrity.”