SBI 3U Genetic Continuity Name: _______________________ Partner’s name: __________________________ Breeding Reebops Objective: To explore genetic variation in a species by constructing baby "Reebops" from two heterozygous parents. Once you have found your partner, and have collected all the materials needed, you may begin to breed your Reebops. Materials: -Complete set of mother chromosomes c) tooth picks -Complete set of father chromosomes d) nails -Materials need for construction of Reebop (number of each material dependent on genotype): e) pipe cleaners f) paper clip pieces a) marshmallows (large and small) g) markers (purple, red, orange, yellow) b) pushpins (coloured and colourless) h) Reebop decoder key Instructions: 1. Find your partner using the images on your envelopes. One partner will take the mother's chromosomes, and the other will take the father's chromosomes. 2. Remove the 16 chromosomes from the envelope. Match up the homologous pairs. A homologous pair will be the same length. You should have 8 pairs when you are done sorting them out. Record your parents' genotype in Table 2. 3. Lay the homologous pairs in a row, face down so the letters are not showing. 4. Look at the longest homologous pair of chromosomes. Choose ONE of the two. Place the rejected one back in the envelope. Do the same for the other chromosome pairs. 5. Place the chosen mother chromosomes along with the chosen father chromosomes in a separate baby pile. This new set will be your Reebop baby's chromosomes. 6. Each Reebop baby should have 16 chromosomes. Match up the homologous pairs. One chromosome in each pair should be from the mother and one should be from the father. Sort these chromosomes according to length to create a karyotype. 7. Predict what your baby's genotype might be and record your predictions in Table 2 (yes it is ok to guess!). 8. Discover your baby's genotype by turning over the chromosomes and record it in Table 2. 9. Discover what your baby looks like using the decoder key in Table 1. Record the phenotype in Table 2. 10. Construct your baby according to your chromosomes, using the materials provided. (DO NOT EAT ANY PART OF YOUR CHILD OR POINTS WILL BE DEDUCTED FROM YOUR LAB). 11. Once your baby is constructed, give it a name. On a piece of paper, write your Reebop baby's name, along with the names of the members in your group. As proud parents you will place your completed Reebop baby in the designated nursery. Table 1. Reebop decoder key. Use the following key to determine the genotype and phenotype of your Reebop parents and baby. Genotype Phenotype Materials used to represent the phenotype TT Tt tt curly tail curly tail straight tail pipe cleaners EE Ee ee 2 eyes 2 eyes 3 eyes purple marker DD Dd dd two body segments two body segments three body segments 2 marshmallows 2 marshmallows 3 marshmallows MM Mm mm 1 hump 2 humps 3 humps small marshmallows red nose orange nose yellow nose red marker orange marker yellow marker LL Ll ll 4 coloured legs 2 coloured, 2 colourless legs 4 colourless legs coloured push pins coloured and colourless push pins colourless push pins AA Aa aa 1 antenna 2 antenna no antenna nails XXd XdXd XdY small ears large ears large ears small paper clip pieces large paper clip pieces large paper clip pieces QQ Qq qq Results Table 2. Genetic variation. Use this table to record your observations. Phenotype Tail Eyes Body segment Humps Nose Legs Antenna Ears Parents' genotype Predict your baby's genotype (Guess) What is your baby's genotype? What is your baby's phenotype? How does this compare to the rest of the class? (examine those in the nursery) Why? Questions 1. Define the following terms using your own words and give examples. Term Meaning Chromosome Example Homologous pair Genotype Phenotype Haploid Diploid Genetic variation 2. Did the baby inherit any traits from the parents? 3. a) When you observed the other babies in the nursery, were any two exactly alike? b) Why do you think they were all different? c) What makes identical twins identical? 4. Were any mutations formed? If so, how could this happen in this activity? 5. What does it mean when an organism is homozygous for a trait or heterozygous for a trait? 6. What is the purpose of meiosis? Ticket out of class: Based on the introduction to genetics given in this activity, what are two ideas or concepts you would like to explore in the Genetic Continuity unit. i.e. Why is diversity important? What would happen to the population of Reebop babies if they were all the same?