REEBOPS LAB: Marshmallow Meiosis Background: Reebops are tiny creatures that live in discarded pop cans and shoe boxes. They are difficult to observe in the wild because they are shy and move very quickly. Reebops are prolific breeders in captivity and thrive with minimal care. They inherit traits in the same way as humans so they are excellent subjects for studying genetics. Chromosomal analysis has revealed that this unusual species has 16 chromosomes (8 homologous pairs). In this activity, you will simulate the reproduction of 2 Reebop parents and construct the resulting offspring. You will use concepts of meiosis to sort the parents’ chromosomes and form their gametes then use the information to determine the baby Reebop’s traits. After the baby Reebops are born, the siblings will be assembled in a nursery so that their traits can be analyzed. Purpose: to model and interpret the role of meiosis in sexual reproduction. Materials: Paper homologous chromosomes (Mom’s set=pink; Dad’s=blue) Large marshmallows, small colored marshmallows, push pins, pipe cleaners, thumbtacks, nails, toothpicks (to attach marshmallows to each other) Procedure: 1. Observe Mom and Dad Reebop and record their phenotypes. A. __________________________________________________________________ __________________________________________________________________ 2. Open the envelope, take out all the cards and turn them face down, keeping Mom’s (pink) and Dad’s (blue) separate as you sort them out. B. How many chromosomes does each parent have? _____________________ 3. Sort each set of chromosomes into pairs of the same length (homologous pairs). C. How many homologous chromosomes does each parent have? ______________ D. Why does each reebop have 2 alleles for any given trait? 4. Randomly take one chromosome from each pair of Mom’s chromosomes and place in a pile. This pile is the ‘female gamete’ (egg cell). E. Is the female gamete diploid or haploid? ___________How do you know? F. What type of cell division makes eggs? __________________ 5. Randomly take one chromosome from each pair of Dad’s chromosomes and place in another pile. This pile is the ‘male gamete’ (sperm cell). G. Is the male gamete diploid or haploid? _____________How do you know? H. What type of cell division makes sperm? __________________ 6. Put the chromosomes you haven’t used back into the envelope. 7. Fertilize the female gamete with the male gamete by mixing together the female and male gamete piles. This is now your selection of ‘baby genes’ (the zygote chromosome set). Sort out the chromosomes of your new individual into homologous pairs. I. How many chromosomes does your zygote have? __________ J. How does this number compare to the number that each parent has? ______ K. Is the zygote haploid or diploid? _____________ L. What has to happen to the zygote next for it to develop into a baby? H. Write down the letters you have obtained in Table 2 for your ‘baby Reebop’. For example, if you have one card with the letter A and another card with the letter a, your genotype is Aa. I. Use the ‘decoder key’ to decide what the phenotype of your baby Reebop will be based on your genotype description. Record in Table 1. J. Collect all the materials you need for your baby Reebop and build your baby Reebop. Join the marshmallows with toothpicks. Draw your baby reebop below. K. Take a look at all the reebops made by your classmates. You should notice that each is different. There are several processes that make genetic diversity possible. i. What process happens in meiosis? ii. How does fertilization increase further genetic diversity? iii. Why are brothers and sisters different even if they have the same parents L. Put your baby in the Reebop nursery with the other newborns! M. In Table 1, star the Reebop traits that show incomplete dominance. Explain why you picked these traits. Table 1: Decoder Key Trait antenna humps nose tail eyes legs body segments sex Genotype AA Aa aa MM Mm mm QQ Qq qq TT or Tt tt EE or Ee ee LL or Ll ll DD or Dd dd XX XY Table 2: Baby Reebop genotype and phenotype Chromosome Alleles Phenotype 1 antenna 2 antenna no antenna 1 green hump 2 green humps 3 green humps red nose orange nose yellow nose curly tail straight tail 2 eyes 3 eyes clear legs colored legs 3 body segments 2 body segments female male Genotype (also record if pure-bred or hybrid) 1 2 3 4 5 6 7 8 Parts used nails colored marshmallows colored marshmallows pipe cleaner silver tacks push pins large marshmallows bow on head or bowtie Phenotype Discussion and Conclusion: Answer in complete sentences! Most of the lab points will come from this section. 1. Reebops have a diploid number (2n) of 18. Explain how the diploid number of chromosomes is maintained from one generation to the next. In other words, why did the baby reebop have 18 chromosomes instead of 36? (4 pts) 2. For any given trait, how many alleles does the baby reebop have? Explain where these alleles came from. (2 pts) 3. Each parent reebop was heterozygous for each trait. Explain what this means. (2 pts) 4. Children from the same parents are not exactly the same. Explain why this is true based on the reebop activity and what you know about meiosis. (4 pts) 5. Fill in the following table: Trait Punnett square to predict % Curly tail Calculate actual % in nursery 3 humps 5. Write a paragraph (5 sentences minimum) to describe your predictions in #5 and explain why they were or were not the same as the actual results in #6. Were the actual results the same as the predictions you made in #5? Explain why or why not. (10 pts)