OIE Collaborating Centre Reports
Activities in 2011
Title of Collaborating Centre:
Address of Collaborating Centre:
New and Emerging Diseases
Australian Animal Health Laboratory
Geelong, Victoria 3220, AUSTRALIA
Tel.:
(61 3) 5227 5000
Fax:
(61 3) 5227 5555
e-mail address:
website:
martyn.jeggo@csiro.au
www.csiro.au
Name of Director of Institute
(Responsible Official):
Dr Martyn Jeggo
Name (including Title and
Position) of Head of the
Collaborating Centre (formally
OIE Contact Point):
Dr Martyn Jeggo
Name (including Title and
Position) of writer of this report
(if different from above)
Dr Peter Daniels
Annual reports of OIE Reference Centres, 2011
1
New and Emerging Diseases
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
1.
Activities as a centre of research, expertise, standardisation and dissemination of techniques within
the remit of the mandate given by the OIE
Research
Research
a.
AAHL is a national laboratory facility for the biocontainment of foreign animal diseases and new and emerging
animal diseases and viral zoonoses, operating at BSL3 (enhanced) and BSL4. It provides a rapid diagnostic
capability and outbreak diagnostic surge capacity through a Diagnosis, Surveillance and Response group and
conducts research in to the major transboundary animal diseases and viral zoonoses under the theme of
transforming animal biosecurity. Work is directed towards 3 main objectives: Reduction of Disease Emergence or
Invasion Risk, Mitigation of Disease Impact through Decision Support for Limiting Disease Spread and Mitigation
of Disease Impact through Modification of the Host Response. This year has seen a considerable enhancement (5
times) of the available PC4 space with the addition of 350 sq metres, including areas for small animals, insects and
live cell imaging. Additionally a new large insectary will be opened by midway through this current year.
b.
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Research dealing specifically with emerging infectious zoonotic diseases includes:
development of novel antiviral treatments for Henipavirus infections including passive immunotherapies
optimization and commercialisation of Hendra vaccine for use in equines
development of an improved diagnostic capability for Henipavirus infections including stall-side tests
use of reverse genetics systems for further henipavirus studies
Use of continuous bat cell lines for host-virus interaction and immunological studies
comparative pathogenesis study on different strains of Hendra and Nipah viruses with a focus on transmission
mechanisms
Further use of the ferret model for henipavirus research and vaccine/therapeutics trials
Studies on Paramyxovirus infections in poultry and in pigeons
pathogenesis studies of SARS Coronavirus in bats
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2
AAHL is continuing to work on AI pathogenesis and studies of AI vaccines and vaccination.
Challenge models of H5N1 infection have been developed in chickens, ducks, ferrets and mice. Vaccine efficacy
testing has continued using a number of commercial vaccines.
Comparative pathogenesis studies are being conducted both with field strains of H5N1 from various countries and
species and with AI viruses constructed using reverse genetics (in collaboration with US and European research
organisations).
Two major studies with an international poultry breeding company have continued; one using transgenic
techniques to develop poultry with an enhanced immune system that will be substantially resistant to AI
infection and another to develop anti-viral therapeutics. Both projects are now well underway and are likely to
substantially achieve their overall objectives.
Wild bird isolates of avian influenza viruses from Australia have been sequenced and the data used to better
understand the movement of these strains both within Australia and in neighbouring countries. .
Epidemiological studies in Indonesia and Vietnam have continued to investigate AI transmission in markets, AI
infection in ducks, and the impact of vaccination on AI transmission in both commercial and village sectors.
d.
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Work on foot and mouth disease includes:
development of new diagnostic approaches and reagents
evaluation of vaccines contained in the Australian FMD bank using challenge studies in Vietnam (in pigs)
Epidemiological studies of effectiveness of foot and mouth disease control programs in Vietnam
Management of classical swine fever and foot and mouth disease at the village level in Laos
e.
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Wildlife disease research is continues on:
the role of Australian bats in Hendra virus maintance
Koi herpesvirus as a potential biological control agent
Annual reports of OIE Reference Centres, 2011
New and Emerging Diseases
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Research on other diagnostic technologies and test development includes
Use of molecular detection and characterisation systems for characterising unknown viruses isolated in Australia
Rapid characterisation of unknown viruses using microarray hybridisation and PCR-select-subtraction and mass
(454) sequencing
Development and production of immunological reagents and tests for emerging infectious diseases using
recombinant antibody technology
Use of diagnostic tests for abalone herpesvirus and applying these in transmission trials and surveillance
Research has commenced on a number of arbovirus infections using newly acquired facilities and expertise in
insects that act as vectors
Studies on the development of a vaccine against ephemeral fever (three day sickness) in cattle
Studies on bluetongue virus isolates from insects in Australia and their full characterisation
Studies on innate immunity in insects
Studies in insect transmission of important arboviruses
Expertise
2.
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In addition to the specific missions by AAHL staff listed under section 3 below, AAHL’s Dr. Linfa Wang serves
as a member of the WHO SARS Reference and Verification Laboratory Network
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With respect to terrestrial animal disease, AAHL is a designated OIE Reference Laboratory for Hendra and Nipah
Virus Diseases, Bluetongue Disease, Newcastle Disease and Avian Influenza. See separate reports.
Proposal or development of any procedure that will facilitate harmonisation of international
regulations applicable to the surveillance and control of animal diseases, food safety or animal
welfare
Through OIE and FAO AAHL has been collaborating in the development of initiatives to harmonize veterinary
laboratory testing for emerging infectious and zoonotic diseases. Training in systems to improve the strategic planning
of laboratory activities within the animal health sector operations are being developed, as well as management systems
that help laboratories fine tune their quality assurance systems and their laboratory biorisk management procedures.
AAHL continues to assist the OIE in developing approaches and standards to diagnostic test validation. Dr Axel Colling
contributed to the OIE ad hoc Group Meeting on validation of diagnostic assays during 2010 as well as discussions of
the recent developments in the area of test validation during an FAO/IAEA/OIE consultants meeting at the IAEA/FAO
Joint Division in Vienna. During 2011 the outcomes of these processes, draft new sections and approaches in the OIE
Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, have been circulated to member countries for their
consideration and responses.
3.
Networking
a)
Maintenance of a network with other OIE Collaborating Centres designated for the same
specialty, and
At the invitation of the OIE AAHL collaborates with other OIE Collaborating Centres including the Center for
Epidemiology and Animal Health, USDA, Fort Collins, the Collaborating Centre for Diseases at the
Animal/Human Interface, IZSV, Padova, the Collaborating Centre for Emerging and Re-Emerging Zoonotic
Diseases at the National Center for Emerging and Infectious Diseases, CDC, Atlanta. These Collaborating Centres
come together for activities such as The Technical Consultation in Support of the OIE Emerging Pandemic Threats
Program held at OIE HQ 25-27 January 2011.
b)
Should the need arise, maintenance of a network with Collaborating Centres in other disciplines
AAHL participates in the Global Outbreak Alert and Response Network (GOARN) and the Emerging and
Dangerous Pathogens Laboratory Network (EDPLN) under the auspices of WHO.
Annual reports of OIE Reference Centres, 2011
3
New and Emerging Diseases
4.
Placement of expert consultants at the disposal of the OIE
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4
Wilna Vosloo participated in meetings as the agreement holder for the International Atomic Energy Agency
Project entitled Development of Molecular and Nuclear Technologies to FMD, Rome, Italy 8-16 Jan 11.
Peter Daniels participated in The 3rd Meeting of OIE Bluetongue Network in Teramo, Italy on 21 January 2011
Martyn Jeggo participated in the Animal Health Stakeholders Awareness Meeting in South East Asia 20-21,
Bangkok Thailand - 19-22 Jan 11
Peter Daniels participated in The Technical Consultation in Support of the OIE Emerging Pandemic Threats
Program in Paris, 25-27 January 2011
Peter Daniels participated the WHO/OIE/FAO International Meeting on Influenza Interspecies Transmission
sponsored by CDC and held in Treviso, Italy, 1-3 February 2011
Glenn Marsh attended the 6th international Conference on Emerging Zoonoses, Cancun, Mexico, 24-27 Feb 11
John Allen participated in the first meeting for the lab strengthening working group for the Australian Indonesia
Partnership for Emerging Infectious Diseases 2010-2014, Jakarta, Indonesia, 21-25 Feb 11.
Sam McCullough participated in the Global Outbreak Alert and Response Network (GOARN) Training, Kampot,
Cambodia, 24 Feb to 5 Mar 11.
Wilna Vosloo was invited by OIE to attend the 17th meeting of the OIE sub-commission for FMD control in SE
Asia, Denpasar, Indonesia, 7-11 Mar 11.
Ross Lunt facilitated the OIE/FAO BTV Workshop in Bogor, Indonesia, 5-11 Mar 11
Linfa Wang was invited as a speaker at the XII International Symposium on Respiratory Viruses, Rome, Italy, 1117 Mar 11
Peter Daniels attended the OIE ad hoc Group Meeting on Epizootic Haemorrhagic Disease, Paris, France, 13-17
Mar 11.
Linfa Wang provided a seminar at the Pasteur Institute, Paris, France 20-23 Mar 11
John Allen attended the OIE twinning Network Meeting, Paris France, 27 Mar to 8 Apr 11
Martyn Jeggo attended meetings with Prof Borriello, Chief Executive AHVLA, to discuss Twinning Projects,
Surrey, UK, 28-29 Mar 11.
Martyn Jeggo was invited to participate in the OIE Twinning Program Workshop, Paris, France, 30-31 Mar 11
Frank Wong attended the OFFLU Technical Meeting – Influenza in Swine, OIE Headquarters Paris, France 5-8
Apr 11.
John Allen attended the OIE workshop on Laboratory Gap Analysis, Paris, France 12-13 May 11
Martyn Jeggo presented at the NFID 2011 Annual Conference on Vaccine Research, Baltimore, MD, USA -14-21
May 11
Greg Smith attended the 14th IVBW Conference in Copenhagen, Denmark, 14-24 May 11.
Paul Selleck and Gemma Carlile visited Regional Laboratories as part of the OIE Twinning Project to establish PT
panel methodology with lab staff in country, Ipoh, Malaysia, 15-28 May 11.
Peter Daniels attended the DISCONTOOLS FP7 project meeting of the Nipah Virus Expert Group, Brussels,
Belgium, 28 May – 1 Jun 11.
Martyn Jeggo was invited to present at the conference for Emerging and Persistent Infectious Diseases: Focus on
Prevention convened by the Institute on Science for Global Policy (ISGP) in San Diego, 03-08 Jun 11
Chris Morrissy was involved meetings to prepare for linking assessment missions and gap analysis in Viet Nam, 519 Jun 11.
Wilna Vosloo attended the Global FMD Research Alliance Annual Workshop in Buenos Aires, Argentina 14-16
Jun 11
Frank Wong and Mai Hlaing Loh conducted sequencing training and to attend a sequence network meeting as part
of the OIE Twinning Project, Ipoh, Malaysia, 20 -25 Jun 11
John Allen and Chris Morrissy were invited to undertake a scoping mission for a Lab-Field Epidemiology
Training Course for FAO, Bangkok, Thailand, 26 Jun to 2 Jul 11
Serge Corbeil was invited to present at the OIE Global Conference on Aquatic Animal Health, Panama City,
Panama, 27 Jun to 1 Jul 11
Wilna Vosloo attended OIE FMD Meeting in Paris, France 27-29 Jun 11
Martyn Jeggo was invited to attend the GREP Symposium and Rinderpest Eradication Declaration, Rome, Italy,
27-28 Jun 11
Martyn Jeggo attended the first meeting of the International Society of One Health (ISOH), London, UK – 29 Jun
11
Frank Wong travelled to Surabaya in Indonesia to provide sequencing training and to attend a sequence network
meeting as part of the OFFLU Project, Indonesia, 3-9 Jul 11
Annual reports of OIE Reference Centres, 2011
New and Emerging Diseases
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Alex Hyatt was invited to present as the keynote speaker at the 1st International Ranvirus Symposium,
Minneapolis, USA, 6-14 Jul 11.
Brian Meehan conducted training for the Diagnosis of CSF and PRRS under the FAO PRRS/CSF project, Ho Chi
Minh City, Viet Nam, 7-24 Jul 11.
Greg Smith was invited to attend the US National Academies Workshop on Anticipating Biosecurity Challenges
of the Global Expansion of High Containment Biological Laboratories, Istanbul, Turkey, 10-15 Jul 11
Gemma Carlile and Paul Selleck conducted follow-up meetings and analysis of test results as part of the OIE
Twinning Project, Ipoh, Malaysia, 24-29 Jul 11.
Peter Daniels presented a plenary paper at the Sustainable Animal Agriculture for Developing Countries
Conference, Bangkok, Thailand, 24-30 Jul 11.
Peter Daniels attended the FAO/OIE Regional Laboratory Network Technical Advisory Group Meeting, Bangkok,
Thailand 2-6 Aug 11
Chris Morrissy was invited to attend the FAO LabNet meeting in Bangkok, Thailand, 4-5 Aug 11.
Greg Smith attended the National Academies Meeting, Washington DC, USA, 4-8 Sept 11.
Nick Moody visited the CEFAS Laboratory to workshop development of professional networks and diagnostic
protocols, UK, 4-9 Sept 11.
Nick Moody attended the EAFP 15th International Conference on Diseases of Fish and Shellfish, Split, Croatia,
10-17 Sept 11.
Martyn Jeggo and Nick Lombardi attended the 4th GOHLD meeting of Directors of High Security Biocontainment Facilities, Winnipeg, Canada, 12-16 Sept 11.
Peter Daniels chaired the OFFLU Steering Committee Meeting and attended the ad hoc Group meeting on
Biosafety and Biosecurity in Veterinary Laboratories, Paris, 14-21 Sept 11
Nick Moody visited the EU Reference Laboratory for Fish Diseases to workshop development of professional
networks and diagnostic protocols, Aarhus, Denmark, 18-23 Sept 11.
John Allen attended the WHO Regional Workshop on Emerging and Dangerous Pathogens Laboratory Network
(EDPLN) in Jakarta, Indonesia, 20-22 Sept 11.
Martyn Jeggo presented at the RELU Conference - Living with uncertainty in animal disease management,
London, UK, 20-22 Sept 11.
Sam McCullough presented an invited paper at the 23rd Malaysian Veterinary Association Annual Meeting, Ipoh,
Malaysia, 23-26 Sept 11.
Nick Moody visited the IFREMER Laboratory to enable development of professional networks and diagnostic
protocols, La Tremblade, France, 25-27 Sept 11.
Martyn Jeggo presented at Hong Kong Agriculture, Fisheries and Conservation Department Workshop - One
Health - The Road Ahead - Hong Kong, 26-28 Sept 11
Chris Morrissy attended FAO meetings at Pirbright to plan the 4 Way Linking Mission initiative, UK, 10 Oct 2011
John Allen attended the Annual International Symposium for Biosecurity and Biosafety: Future Trends and
Solutions in Milan, 12-14 Oct 11
Martyn Jeggo was invited to participate in the preparation meeting for PMAC 2013 One Health - Bangkok, 15-17
Oct 11
Chris Morrissy attended a 4 Way Linking Meeting as the OIE representative with FAO and WHO, Hanoi,
Vietnam, 15-19 Oct 11
Chris Morrissy and Gemma Carlile attended the OIE meeting on Laboratory Strengthening in Kuala Lumpur,
Malaysia, 19-21 Nov 11.
Martyn Jeggo participated in discussions on One Health Governance & the development of a One Health Global
Network in Atlanta, 30 Oct – 03 Nov 11
Wilna Vosloo attended OIE/FAO meetings at Pirbright UK and the OIE FMD Committee Meeting in Paris, 11-26
Nov 11
Chris Morrissy travelled to Beijing to provide a back stopping mission under the FAO PRRS/CSF project, Beijing,
China 20-27 Nov 11
Peter Durr and Paul Selleck visited Putsvetma to continue work under OFFLU Indonesia project, Surabaya,
Indonesia, 1- 8 Dec 11.
Jianning Wang attended the Integrating Sample Preparation 2011 International Conference: Integrating Sample
Preparation of Chemical and Biological Agents, Threats and Pathogens into Detection, identification and Analysis
Technologies and Devices, Washington DC, USA, 6-10 Dec 11.
John Allen, James Watson and Lynda Wright attended the OIE/FAO Laboratory Information Management System
(LIMS) Development Workshop, Phuket, Thailand 6-10 Dec 11
Martyn Jeggo attended the IOM Meeting on One Health in Washington as a keynote speaker at the Institute of
Medicine (IOM), 12 Dec – 16 Dec 11
Annual reports of OIE Reference Centres, 2011
5
New and Emerging Diseases
5.
Provision of scientific and technical training, within the remit of the mandate given by the OIE, to
personnel from OIE Member Countries
AAHL has delivered substantial training in the area of new and emerging infectious disease. These activities have been
comprehensively covered in the report of the Collaborating Centre for Laboratory Capacity Building and in the reports
of the Avian Influenza and Hendra and Nipah Virus Diseases reference laboratories.
6.
Organisation of scientific meetings on behalf of the OIE
AAHL has membership on the Scientific & Technical Advisory Board of the GRF One Health Summit 2012,
One Health - One Planet - One Future. Risks and Opportunities, scheduled for 19-22 February 2012 in Davos,
Switzerland.
A workshop was held in Ho Chi Minh City to help develop regional protocol for optimisation of standard PCR assay
and perform optimisation of PCR assays for CSF and PSSR using platform available in Australia for South East Asia
and China.
7.
Coordination of scientific and technical studies in collaboration with other laboratories,
organisations or collaborating centres
Collaborative research with other scientific agencies is a core activity for AAHL. Details of current projects have been
presented in Section 1 of this report
8.
Publication and dissemination of any information within the remit of the mandate given by the OIE
that may be useful to Member Countries of the OIE
Journal Articles
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6
Adler B, Rood J, Gaastra W, Browning G, Moore R. Preface: Pathogenesis of bacterial diseases of animals.
Veterinary Microbiology. 2011;153(1-2):1-.
Barrett S, Mohr PG, Schmidt PM, McKimm-Breschkin JL. Real Time Enzyme Inhibition Assays Provide Insights
into Differences in Binding of Neuraminidase Inhibitors to Wild Type and Mutant Influenza Viruses. PLoS One.
2011;6(8).
Bowden TR, Boyd V, Felgner PL, Davies DH. Enhancing the Capability for Pathogen Detection and Surveillance
Using Protein Microarrays. EcoHealth. 2011;7:S42.
Boyd, Vicky, Foord, Adam, Heine, Hans. Detection and Differentiation of Avian and Zoonotic Pathogens by a
Luminex Liquid Bead Array Multiplex Assay. EcoHealth 2011;7:S140
Brown L, Cobbin J, Middleton D, Barr I, Zeng W, Jackson D. Universal vaccination: is this premature with
current vaccine approaches? Influenza and other Respiratory Viruses. 2011;5:15-8.
Burggraaf S, Bingham J, Payne J, Kimpton WG, Lowenthal JW, Bean AG. Increased inducible nitric oxide
synthase expression in organs is associated with a higher severity of H5N1 influenza virus infection. PLoS One.
2011;6(1):e14561.
Chua KB, Voon K, Yu M, Ali W, Kasri AR, Wang L-F. Saffold Virus Infection in Children, Malaysia, 2009.
Emerging Infectious Diseases. 2011;17(8):1562-4.
Chua KB, Voon K, Yu M, Keniscope C, Rasid KA, Wang L-F. Investigation of a Potential Zoonotic Transmission
of Orthoreovirus Associated with Acute Influenza-Like Illness in an Adult Patient. PLoS One. 2011;6(10).
Clayton B, Haining J, Robinson R, Middleton D, Wang L-F, Marsh G. Nipah Viruses from Malaysia and
Bangladesh: Differences in Transmission and Pathogenesis. Ecohealth. 2011;7:S140-S1.
Cowled C, Baker M, Tachedjian M, Zhou P, Bulach D, Wang LF. Molecular characterisation of Toll-like
receptors in the black flying fox Pteropus alecto. Developmental and Comparative Immunology. 2011;35(1):718.
Crowley TM, Haring VR, Moore R. Chicken anemia virus: an understanding of the in-vitro host response over
time. Viral Immunology. 2011;24(1):3-9.
Cummins DM, Tyack SG, Doran TJ. Characterisation and comparison of the chicken H1 RNA polymerase III
promoter for short hairpin RNA expression. Biochemical and Biophysical Research Communications. 2011.
Annual reports of OIE Reference Centres, 2011
New and Emerging Diseases
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Daniels P, Hamilton K, Dauphin G, Mumford E, Swayne D, Edwards S. OFFLU, A Novel International
Partnership Approach to a Major Zoonotic Threat. EcoHealth. 2011;7:S122
Dups J, Haining J, Robinson R, Wang L-F, Middleton D, Marsh G. Henipaviruses and Mice - Are They Truly
Resistant to Infection? EcoHealth. 2011;7:S141-S.
Durr, Peter Emerging and re-emerging diseases of wildlife and domestic animals – redefining the role of climate
change. EcoHealth 2011;7:S74
Eagles, Debbie, Durr, Peter, Walker, Pter, Zalucki, Myron, Hall, Roy, Deveson, Ted. Modelling the airborne
spread of vectors and insect pests into Northern Australia within a risk analysis framework. EcoHealth
2011;7:S142
Edenborough K, Yamada M, Lowther S, Bingham J, Haining J, Gilbertson B, et al. Route of H5N1 exposure in
ferrets affects pathogenesis and severity of disease. Influenza and Other Respiratory Viruses. 2011;5:87-9.
Everett, Helen, Crooke, Helen, Gurrala, Rajesh, Dwarka, R., Kim, J., Botha, B., et al. Experimental infection of
Warthogs (Phacochoerus africanus) and Bushpigs (Potamochoerus larvatus) with Classical Swine Fever Virus I:
susceptibility and transmission. Transboundary and Emerging Diseases. 2011; 58(2):128-134.
Foord AJ, Selleck P, Colling A, Klippel J, Middleton D, Heine HG. Real-time RT-PCR for detection of equine
influenza and its evaluation using samples from horses infected with A/equine/Sydney/2007 (H3N8). Australian
Veterinary Journal. 2011;89 Suppl 1:37-8.
Foord, Adam, Boyd, Victoria, Heine, Hans A loop mediated isothermal amplification (LAMP) assay for detection
of Hendra virus (HeV) in the field. EcoHealth 2011;7:S143
Gubala A, Davis S, Weir R, Melville L, Cowled C, Boyle D. Tibrogargan and Coastal Plains rhabdoviruses:
genomic characterization, evolution of novel genes and seroprevalence in Australian livestock. Journal of
General Virology. 2011;92(Pt 9):2160-70.
Halpin K, Hyatt AD, Fogarty R, Middleton D, Bingham J, Epstein JH, et al. Pteropid Bats are Confirmed as the
Reservoir Hosts of Henipaviruses: A Comprehensive Experimental Study of Virus Transmission. American
Journal of Tropical Medicine and Hygiene. 2011;85(5):946-51.
Hayman DTS, Wang L-F, Barr J, Baker KS, Suu-Ire R, Broder CC, et al. Antibodies to Henipavirus or HenipaLike Viruses in Domestic Pigs in Ghana, West Africa. PLoS One. 2011;6(9).
Holyoake PK, Kirkland PD, Davis RJ, Arzey KE, Watson J, Lunt RA, et al. The first identified case of pandemic
H1N1 influenza in pigs in Australia. Australian Veterinary Journal. 2011;89(11):427-31.
Islam MS, Kouzani AZ, Dai XJ, Michalski WP. Investigation of the effects of design parameters on sensitivity of
surface plasmon resonance biosensors. Biomedical Signal Processing and Control. 2011;6(2):147-56.
Janardhana V, Tachedjian M, Crameri G, Cowled C, Wang LF, Baker ML. Cloning, expression and antiviral
activity of IFNgamma from the Australian fruit bat, Pteropus alecto. Developmental and Comparative
Immunology. 2011.
Karpala AJ, Bingham J, Schat KA, Chen LM, Donis RO, Lowenthal JW, et al. Highly pathogenic (H5N1) avian
influenza induces an inflammatory T helper type 1 cytokine response in the chicken. Journal of Interferon &
Cytokine Research. 2011;31(4):393-400.
Karpala AJ, Lowenthal JW, Bean AG. Identifying innate immune pathways of the chicken may lead to new
antiviral therapies. Veterinary Immunology and Immunopathology. 2011.
Karpala AJ, Stewart C, McKay J, Lowenthal JW, Bean AG. Characterization of chicken Mda5 activity: regulation
of IFN-beta in the absence of RIG-I functionality. Journal of Immunology. 2011;186(9):5397-405.
Kim LM, Dauphin G, Wong F, Selleck P, Daniels P, Swayne D, et al. OFFLU: Contributions Toward Improved
Vaccine Strain Selection for Poultry. EcoHealth. 2011;7:S63
Kirkland PD, Davis RJ, Wong D, Ryan D, Hart K, Corney B, et al. The first five days: field and laboratory
investigations during the early stages of the equine influenza outbreak in Australia, 2007. Australian Veterinary
Journal. 2011;89:6-10.
Kittelberger R, McFadden AMJ, Hannah MJ, Jenner J, Bueno R, Wait J, et al. Comparative evaluation of four
competitive/blocking ELISAs for the detection of influenza A antibodies in horses. Veterinary Microbiology.
2011;148(2-4):377-83.
Li Z, Xu J, Patel J, Fuentes S, Lin Y, Anderson D, et al. Function of the Small Hydrophobic Protein of J
Paramyxovirus. Journal of Virology. 2011;85(1):32-42.
Lynch M, Duignan PJ, Taylor T, Nielsen O, Kirkwood R, Gibbens J, et al. Epizootiology of Brucella infection in
Australian fur seals. Journal of Wildlife Diseases. 2011;47(2):352-63.
Mackenzie JS, Jeggo MH. 1st International One Health Congress. EcoHealth. 2011;7:S1-S2.
Marsh G, Middleton D. Ebola Reston Virus Infection of Pigs: Disease Significance and Transmission Potential.
EcoHealth. 2011;7:S80
Marsh GA, Haining J, Robinson R, Foord A, Yamada M, Barr JA, et al. Ebola Reston virus infection of pigs:
clinical significance and transmission potential. The Journal of Infectious Diseases. 2011;204 Suppl 3:S804-9.
Annual reports of OIE Reference Centres, 2011
7
New and Emerging Diseases
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8
Middleton D. Update on Hendra virus vaccine. Australian Equine Veterinarian. 2011;30(2):58.
Middleeton, Deborah Emerging virus infections of bats: old relationships, new paradigms EcoHealth 2011;7:S39
Miller DS, Finnie J, Bowden TR, Scholz AC, Oh S, Kok T, et al. Preclinical efficacy studies of influenza A
haemagglutinin precursor cleavage loop peptides as a potential vaccine. Journal of General Virology.
2011;92:1152-61.
Mohr PG, Geyer H, McKimm-Breschkin JL. Mixed influenza A and B infections complicate the detection of
influenza viruses with altered sensitivities to neuraminidase inhibitors. Antiviral Research. 2011;91(1):20-2.
Monahan, Paul, Darpel, Karin, Shaw, Andrew, Green, Dianne, Adams, Mat, Walker, Peter. Cellular Pathogenesis
of Bluetongue Virus: should we be looking at the host or the insect vector? EcoHealth 2011;7:S35.
Oakey J, Hawkesford T, Smith C, Hewitson G, Tolosa X, Wright L, et al. Validation of an influenza virus A 5 '
Taq nuclease assay for the detection of equine influenza virus A RNA in nasal swab samples. Australian
Veterinary Journal. 2011;89:39-42.
Palacios G, Cowled C, Bussetti AV, Savji N, Weir R, Wick I, et al. Rapid Molecular Strategy for Orbivirus
Detection and Characterization. Journal of Clinical Microbiology. 2011;49(6):2314-7.
Pallister J, Middleton D, Wang LF, Klein R, Haining J, Robinson R, et al. A recombinant Hendra virus G
glycoprotein-based subunit vaccine protects ferrets from lethal Hendra virus challenge. Vaccine.
2011;29(34):5623-30.
Pallister, Jackie, Middleton, Deborah, Wang, Linfa, Broder, Christopher. Henipavirus vaccine development.
Journal of Bioterrorism & Biodefense. 2011; 1-8.
Payne J, Yamada M, Rookes J, Bingham J, Middleton D. Neuropathology during Henipavirus convalescence:
resolution of infection or virus persistence? Ecohealth. 2011;7:S148
Penrith ML, Vosloo W, Mather C. Classical Swine Fever (Hog Cholera): Review of Aspects Relevant to Control.
Transboundary and Emerging Diseases. 2011;58(3):187-96.
Reinato Stynen AP, Lage AP, Moore RJ, Rezende AM, da Silva de Resende VDA, Ruy PdC, et al. Complete
Genome Sequence of Type Strain Campylobacter fetus subsp venerealis NCTC 10354(T). Journal of
Bacteriology. 2011;193(20):5871-2.
Rockman S, Maher D, Middleton D. The use of hyperimmune serum for severe influenza infections. Critical
Care Medicine. 2011.
Ruiz, Jeronimo, D'Afonseca, Vívian, Moore, Rob, Azevedo, Vasco. Evidence for reductive genome evolution and
lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains. PLoS One. 2011;
6(4):e18551.
Rutowska, Daria, Meyer, Quintin, Maree, Francois, Vosloo, Wilna, Fick, Wilma, Huismans, Henk. The use of
soluble African horse sickness viral protein 7 as an antigen delivery and presentation system. Vaccine. 2011;
156(1-2):35-48.
Schmidt PM, Attwood RM, Mohr PG, Barrett SA, McKimm-Breschkin JL. A Generic System for the Expression
and Purification of Soluble and Stable Influenza Neuraminidase. PLoS One. 2011;6(2).
Smith I, Broos A, de Jong C, Zeddeman A, Smith C, Smith G, et al. Identifying Hendra virus diversity in pteropid
bats. PLoS One. 2011;6(9):e25275.
Smith NH, Berg S, Dale J, Allen A, Rodriguez S, Romero B, et al. European 1: A globally important clonal
complex of Mycobacterium bovis. Infection Genetics and Evolution. 2011;11(6):1340-51.
Stevens Vicky, Pant Ganesh, Certoma Andrea, Lunt Ross, Wong Frank, Vosloo Wilna. Molecular
Characterization of Rabies Virus from Nepal. EcoHealth. 2011;7:S150
Stewart CR, Bagnaud-Baule A, Karpala AJ, Lowther S, Mohr PG, Wise TG, et al. Toll-Like Receptor 7 Ligands
Inhibit Influenza A Infection in Chickens. Journal of Interferon & Cytokine Research 2011.
Stewart CR, Karpala AJ, Lowther S, Lowenthal JW, Bean AG. Immunostimulatory motifs enhance antiviral
siRNAs targeting highly pathogenic avian influenza H5N1. PLoS One. 2011;6(7):e21552.
Stone BM, Blyde DJ, Saliki JT, Blas-Machado U, Bingham J, Hyatt A, et al. Fatal cetacean morbillivirus infection
in an Australian offshore bottlenose dolphin (Tursiops truncatus). Australian Veterinary Journal.
2011;89(11):452-7.
Sun E-C, Ma J-N, Liu N-H, Yang T, Zhao J, Geng H-W, et al. Identification of two linear B-cell epitopes from
West Nile virus NS1 by screening a phage-displayed random peptide library. BMC Microbiology. 2011;11.
Sun E-C, Zhao J, Yang T, Liu N-H, Geng H-W, Qin Y-L, et al. Identification of a conserved JEV serocomplex Bcell epitope by screening a phage-display peptide library with a mAb generated against West Nile virus capsid
protein. Virology Journal. 2011;8.
Verity EE, Camuglia S, Agius CT, Ong C, Shaw R, Barr I, et al. Rapid generation of pandemic influenza virus
vaccine candidate strains using synthetic DNA. Influenza and Other Respiratory Viruses. 2011.
Virtue Elena, Marsh Glenn, Wang LinFa. Modulation of Interferon Response by Henipavirus Infection.
EcoHealth. 2011;7:S153
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Virtue ER, Marsh GA, Baker ML, Wang LF. Interferon production and signaling pathways are antagonized during
henipavirus infection of fruit bat cell lines. PLoS One. 2011;6(7):e22488.
Virtue ER, Marsh GA, Wang LF. Interferon signaling remains functional during henipavirus infection of human
cell lines. Journal of Virology. 2011;85(8):4031-4.
Vosloo W, Swanepoel SP, Bauman M, Botha B, Esterhuysen JJ, Boshoff CI, et al. Experimental Infection of
Giraffe (Giraffa camelopardalis) With SAT-1 and SAT-2 Foot-and-Mouth Disease Virus. Transboundary and
Emerging Diseases. 2011;58(2):173-8.
Walker PJ, Dietzgen RG, Joubert DA, Blasdell KR. Rhabdovirus accessory genes. Virus Research. 2011;162(12):110-25.
Walpita P, Barr J, Sherman M, Basler CF, Wang L. Vaccine Potential of Nipah Virus-Like Particles. PLoS One.
2011;6(4).
Wang J, Lunt R, Ha W, Walker S. Detection and Differentiation of Flaviviruses Using Real-Time PCR and High
Resolution Melting Curve (HRM) Analysis. EcoHealth. 2011;7:S153
Wang, L-F, Crameri, Gary Bats and SARS Coronavirus. EcoHealth 2011;7:S44
Wang, L-F, Barr, Jennifer, Crameri, Gary Virus discovery using bat cell lines. EcoHealth 2011;7:S45
Wang L-F. Henipavirus: From Emergence to Control. EcoHealth. 2011;7:S41
Wang LF. Discovering novel zoonotic viruses. New South Wales Public Health Bulletin. 2011;22(5-6):113-7.
Watson, James, Bowers, Andrew, Wright, Lynda Can my database talk to your database? Why data standards
matter , but implementing them matters even more. EcoHealth 2011;7:S56
Watson J, Daniels P, Kirkland P, Carroll A, Jeggo M. The 2007 outbreak of equine influenza in Australia: lessons
learned for international trade in horses. Revue Science et Technique. 2011;30(1):87-93.
Watson J, Halpin K, Selleck P, Axell A, Bruce K, Hansson E, et al. Isolation and characterisation of an H3N8
equine influenza virus in Australia, 2007. Australian Veterinary Journal. 2011;89 Suppl 1:35-7.
Watson J, Selleck P, Axell A, Bruce K, Taylor T, Heine H, et al. Diagnosis of equine influenza virus infections in
quarantine stations in Australia, 2007. Australian Veterinary Journal. 2011;89 Suppl 1:4-6.
Wibawa H, Henning J, Wong F, Selleck P, Junaidi A, Bingham J, et al. A molecular and antigenic survey of H5N1
highly pathogenic avian influenza virus isolates from smallholder duck farms in Central Java, Indonesia during
2007-2008. Virology Journal. 2011;8:425.
Windsor M, Hawes P, Monaghan P, Snapp E, Salas ML, Rodriguez JM, et al. Mechanism of Collapse of
Endoplasmic Reticulum Cisternae During African Swine Fever Virus Infection. Traffic. 2011.
Wong, Frank, Deng, Yi-Mo. Pandemic H1N1 2009 influenza in pigs in Australia. Microbiology Australia. 2011;
32(1):29-41.
Wong, Frank, Daniels, Peter, Komadina, Naomi, Barr, Ian, Harrower, Bruce, Deng, Yi-Mo Characterisation of
A(H1N1) 2009 Pandemic Influenza Viruses from across the Human-Animal interface in Australian Swine Herds
EcoHealth 2011;7:S31.
Wynne JW, Bull TJ, Seemann T, Bulach DM, Wagner J, Kirkwood CD, et al. Exploring the zoonotic potential of
Mycobacterium avium subspecies paratuberculosis through comparative genomics. PLoS One. 2011;6(7):e22171.
Yuan J, Marsh G, Khetawat D, Broder CC, Wang L-F, Shi Z. Mutations in the G-H loop region of ephrin-B2 can
enhance Nipah virus binding and infection. Journal of General Virology. 2011;92:2142-52.
Zhao JH, Michalski WP, Williams C, Li L, Xu H-S, Lamb PR, et al. Controlling cell growth on titanium by
surface functionalization of heptylamine using a novel combined plasma polymerization mode. Journal of
Biomedical Materials Research Part A. 2011;97A(2):127-34.
Zhou P, Cowled C, Marsh GA, Shi Z, Wang LF, Baker ML. Type III IFN receptor expression and functional
characterisation in the pteropid bat, Pteropus alecto. PLoS One. 2011;6(9):e25385.
Zhou P, Cowled C, Todd S, Crameri G, Virtue ER, Marsh GA, et al. Type III IFNs in pteropid bats: differential
expression patterns provide evidence for distinct roles in antiviral immunity. Journal of Immunology.
2011;186(5):3138-47.
Conference papers/Conference proceedings
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Burggraaf, Simon, Bingham, John, Kimpton, Wayne, Bean, Andrew. High levels of cytokines are associated with
increased H5N1 influenza severity in chickens when compared to ducks. In: Infection and Immunity Lorne 2011;
16-18 February 2011; Lorne. Lorne, Vic.: The Conference; 2011.
Corbeil, Serge, McColl, Ken, Williams, Nette, Mohammad, Ilhan, Bergfeld, Jemma, Hyatt, Alex, et al. Early
detection of herpesvirus infection in Australian abalone and susceptibility of the virus to chemical treatments. In:
1st FRDC Australasian Scientific Conference on Aquatic Animal Health; 5-8 July 2011; Cairns, Qld, Australia.
FRDC; 2011. Presentation.
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Corbeil, S., Williams, L.M., McColl, K.A., Crane, M. St.J. Biotechnology and the diagnosis and surveillance of
aquatic animal pathogens. OIE Global Conference on Aquatic Animal Health Programmes their benefits for global
food security Panama City, Panama 28-30 June, 2011
Cowled, Chris, Baker, Michelle, Wang, Linfa. Toll-like receptors and RIG-I-like helicases in the fruit bat,
Pteropus alecto. In: Lorne Infection and Immunity; 16-18 February 2011; Lorne, Vic. Lorne, Vic.: Victorian
Infection and Immunity Network; 2011. 63.
Cowley, J.A., Corbeil, S., Chen, H., Bulach, D., Wong, F., Moody, N.J., Ellard, K., Fegan, M., Savin, K., Warner,
S., Crane, M. St. J. Sequence variations amongst abalone herpes-like virus (AbHV) strains provide insights into its
origins in Victoria and Tasmania. 1st Australasian Scientific Conference on Aquatic Animal Health, Cairns, Qld. 58 July, 2011
Hyatt, A.D, Working in s PC2-PC3 Biological Electron an Advanced Microcopy Workshop BIO21 Parkville,
Vic.: University of Melbourne, 2011
Jenkins, Kristie, Keyburn, Anthony, Doran, Tim, Bean, Andrew, Tizard, Mark. Modification of the chicken
immune system using microRNAs. In: Lorne Infection and Immunity; 16th -18th February, 2011; Lorne, Vic.
Lorne, Vic.: Victorian Infection and Immunity Network; 2011. 1.
Kumar, Amit, Moore, Rob, Haring, Volker, Tizard, Mark. Comparison of highly conserved and non conserved
mature microRNAs sequences and their targets. In: Thomas Preiss, editor/s. Lorne Genomes; 13-15th Feb 2011;
Lorne, Vic.: The Conference; 2011. a367, p152.
Marsh, Glenn, Wang, Linfa. Bats - a mixed bag of new and emerging viruses. In: 6th International conference on
emerging zoonoses; 24th-27th February, 2011; Cancun, Mexico. Mexico: The Conference; 2011. 1.
Ng, Justin, Belov, Katherine, Wang, Linfa, Baker, Michelle. The major histocompatibility complex (mhc) of the
black flying fox (pteropus alecto). In: Lorne Infection and Immunity; 16th - 18th February, 2011; Mantra Erskine
Beach Resort, Lorne, Vic. Lorne, Vic.: The Conference; 2011. 1.
Peck, G.R., Shiell, B.J., Bowden, T.R., Michalski , W.P. Increased bacterial cell density and recombinant protein
yield using a novel microbial cultivation system. 36th Lorne Conference on Protein Structure and Function: Lorne,
Vic: 6-10 2011 February.
Wong, Frank, Selleck, Paul, Morrissy, Chris, Allen, John, Daniels, Peter. Recent H5N1 activity and diversification
in commercial poultry in the Southeast Asian region. In: 7th Australian Influenza Symposium; 6th-7th October
2011; Bio21 Molecular Science and Biotechnology Institute, Melbourne. Melbourne, Vic.: WHO Collaborating
Centre for Reference and Research on Influenza, VIDRL; 2011. 1
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