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Supplementary files
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Supplementary file S1 (Format: DOC) – Calibration of microsatellite genotyping between
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the BaseStation 51TM and Beckmann Coulter CEQ 8000 genotyping platforms
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Further details describing calibration procedures applied to test scoring consistency and actions
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taken to assess potential risk of compiling genotypes obtained by different platforms and
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persons.
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Supplementary file S2 (Format: XLS) – Pairwise estimates of FST and ΦST for the mtDNA
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marker
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Pairwise estimates of FST (based on haplotype frequencies) and the estimates of ФST reported in
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Debes et al. (2008) for all pairwise mtDNA samples.
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Supplementary file S3 (Format: XLS) – Pairwise estimates of FST and RST for the
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microsatellite markers
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Pairwise estimates of FST and RST including 95% CI among all samples.
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Supplementary file S4 (Format: XLS) – Summary statistics for all microsatellite loci and
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samples
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For each locus and sample are given, i) number of alleles ii) allelic richness iii) expected
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heterozygosity iv) observed heterozygosity v) p-value for test for conformance to Hardy-
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Weinberg Equilibrium and vi) Weir and Cockerhams (1984) inbreeding coefficient.
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Supplementary file S5 (Format: PDF) – Outlier test for microsatellites
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Log-transformed Bayes factors and locus-specific FST values from the BayeScan test. Vertical
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lines show Log10(BF) of 1.5 (black) and 2.0 (red) corresponding to posterior probabilities that a
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single locus is an outlier of 0.97 and 0.99, respectively. Two loci (Spsp202 and Spsp275) with a
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posterior probability of 1 were assigned a Log10(BF) of 5. These two loci were also outliers in
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the test implemented in Arlequin.
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Supplementary file S6 (Format: XLS) – Power analyses
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POWSIM analyses for testing power to detect genetic differentiation at a range of FST values for
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mtDNA and eight microsatellite loci respectively. Four scenarios corresponding to
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differentiation across the four defined transition zones were simulated (see main text for more
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details).
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Supplementary file S7 (Format PDF) – Single-locus based overall RST permutation tests
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Observed overall RST estimates (black squares) for each microsatellite locus and for all loci
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compiled. Open squares and vertical bars represent mean and 95% CI of the ρRST distribution
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obtained by randomly permuting over allelic states for each locus (see text for more details).
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Asterisks denote comparisons where allele sizes (i.e. mutation) explain a significant part of the
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genetic differentiation.
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