OASIS Biotechnology Equipment Loan Form
Welcome! OASIS (housed at Athens Technical College) is very excited about your participation
in the biotechnology equipment loan program. We no longer have funding for Bio Rad kits so
you will have to order the kits on your own. We still have the in-house DNA, Bacterial
Antibiotic Resistance, and Biofuel kits available. When filling out the form below, please give us
a few date choices to allow for flexible scheduling since availability of the equipment can be
affected by delays in equipment returns.
Our new web site is: www.ATCBiotech.org.
We look forward to working with you.
Jeff
Date Request Received
Date Equipment Sent
Contact information:
Date Equipment Returned
Jeff Rapp, Ph.D.
Biotechnology Program Chair
Athens Technical College
800 U.S. Hwy 29 N
Athens, GA 30601-1500
jrapp@athenstech.edu
# of students/class
PCR Machine (circle) has
need
Your Information:
In order for me to process your equipment/kit requests I need the following information. Also,
our grants require us to maintain information of our impact on students and teachers, so please
fill in class type and # of students in each class that are going to be using our kits/equipment.
Thank you
Name: ________________________
email address: ______________________
School name: ___________________________________________________________
School address: _________________________________________________________
School phone: __________________________________________________________
Will other teachers be using this kit with you? If so:
Name:
Name:
Name:
Name:
________________________
________________________
________________________
________________________
email address: ______________________
email address: ______________________
email address: ______________________
email address: ______________________
1
n/a
Students and classes equipment will be used in:
Number of students/class: __________ Course ____________________Teacher___________
Number of students/class: __________ Course ____________________ Teacher___________
Number of students/class: __________ Course ____________________ Teacher___________
Number of students/class: __________ Course ____________________ Teacher___________
Number of students/class: __________ Course ____________________ Teacher___________
We recommend the following Bio Rad kits to start with:
(To order the Bio Rad kits go to: www.explorer.bio-rad.com or contact Andrew_Greer@bio-rad.com (Georgia Bio
Rad Rep))
Please check which kits you will be using and approximate dates for lab.
Note: All kits serve 8 stations of 2-4 students-(the ELISA 12 stations of 2-4 students)
Date
____ELISA Immuno Explorer Kit (166-2400EDU-$120.00 or refill 166-2401EDU-$86.78) ___________
____pGLO Bact.Transform. Kit (166-0003EDU-$89.00 or refill 166-0555EDU- $51.68) _____________
____Rest. Digest & Analysis of Lambda DNA Kit (166-0002EDU-$99.00)
_______________
or refill (166-0012EDU-$77.03)
____Biofuel Enzyme kit (166-5035EDU- $125.00)
_______ Other _______________________________________
_______ Other _______________________________________
_______________
_______________
_______________
Bio Rad Advanced Kits-(require prior training to do PCR)
(To order the Bio Rad kits go to: www.explorer.bio-rad.com or contact Andrew_Greer@bio-rad.com (Georgia Bio
Rad Rep)) Note:
All kits serve 8 stations of 2-4 students.
_____PV92 PCR Informatics Kit (DNA from hair or cheek cells)
______________
(166-2100EDU, $199.00 or refill 166-2119EDU, $97.00 + need protease solution 166-2003EDU, $33.00)
_____GMO Investigator Kit (166-2500EDU, $175.00 or refill 166-2501EDU, $118.00) ___________
_____Comparative Proteomics Kit (166-2700EDU $165.00 refill 166-2701EDU $69.00) ____________
Note: you will also need to order Mini Protean TGX pre-cast polyacrylamide gels 456-1093EDU; $74.40 (10 gels).
OASIS In-house kits: These kits are free-(Note: Kits are assembled based on # of students
you report / lab).
_______Micro-pipetting Exercise
_______Cheek Cell DNA kit
_______Antibiotic Resistance
_______ Biofuel Lab
# students_________
# students_________
# students_________
# students_________
2
_______________
_______________
_______________
_______________
Equipment needs for each lab:
(Note: Please place an X by equipment that you need. OASIS will determine your exact
equipment #s (ex: 2 vs. 4 gel rigs) based on the largest number of students you reported for
classes listed above.) If you already have equipment but need an extra set of pipets, etc., just
indicate # needed.
ELISA Immuno Explorer Kit:
___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
___ Pipet tips 1000 µl & 200 µl (box of 96 ea)
pGLO Bacterial Transformation Kit:
___ UV light
___ 37ºC incubator oven
___ Water bath
___ Pipet tips 1000 µl & 200 µl (box of 96 ea)
___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
Restriction Digest & Analysis of Lambda DNA Kit:
___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
____ Gel rigs (complete with comb and gel tray)
___ Power supply
___ Pipet tips 1000 µl & 200 µl (box of 96 ea)
___ Micro centrifuge
___ Water bath
___ Light Box
Comparative proteomics kit (fish muscle protein kit):
___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
____ Pipet tips 1000 µl & 200 µl (box of 96 ea)
____ Power supply
____ Water bath
____ Gel rigs (complete with comb and gel tray) or
____ Protein polyacrylamide rigs (need pre-cast gels)
____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
____ Low melt agarose
____ Acetic acid 100 ml
____ Alcohol/ethanol 400 ml
Biofuel Enzyme kit:
____ Spectrophotometer
_____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
3
_____
_____
_____
_____
_____
_____
Lab tape
Mortar & pestles
Water bath
Incubation oven
Mini centrifuge
Pipet tips 1000 µl & 200 µl (box of 96 ea)
The following kits require prior experience with PCR:
PV92 PCR Informatics kit:
___ Gel rigs (complete with comb and gel tray)
____ Pipet tips 1000 µl & 200 µl (box of 96 ea)
____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
____ Power supply
____ Thermal cycler
____ Micro centrifuges
____ Water bath
____ Pipet tips with aerosol barriers
____ Light box
GMO Investigator kit:
____ Gel rigs (complete with comb and gel tray)
_____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul)
_____ Pipet tips with aerosol barriers
_____ Thermal cycler
_____ Power supply
_____ Mortar pestle
_____ Light box
_____ Micro centrifuge
OASIS In- House produced kits:
Micro-pipetting exercise kit:
___ Adjustable volume micro-pipets (P20, P200 & P1000)
____ 96 well plates
____ 15 ml tubes of colored water- 1 each of blue, green, yellow & red.
Cheek Cell DNA kit:
____ Transfer pipettes
____ 15 ml tubes with water
____ DNA extraction buffer
____ 90% ETOH
4
____ Paper towels
____ Macro Pipettor
Antibiotic Resistance kit
____ 70% ETOH
____ Agar
____ Petri plates / 1 per student
____ Antibiotic discs
____ Sterile cotton swabs
____ Gloves
____ Broth tubes, 1 per student.
____ Agar slant bacterial cultures, 1 per 4 students.
____ Biohazard bag
Biofuel Lab
___ Heated stir plates and stir bars.
____ 250 ml separatory flask and support ring.
Other equipment available:
___ pH meters
___ TI 83 graphing calculator (2 - available)
____ Portable balances max 1500 g (2 - available)
____ Heating stir plates (14 available)
____ Spectrophotometer (1 - available)
____ Portable balances (2 - available)
____ Vernier labPro- (6 - available)
____ Vernier temp probes (6 - available)
____ Lab timers (8 - available)
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