OASIS Biotechnology Equipment Loan Form (Partially Funded by a National Science Foundation LISELL-B grant with the UGA Dept. of Education) Welcome! OASIS (housed at Athens Technical College) is very excited about your participation in the biotechnology equipment loan program. We no longer have funding for Bio Rad kits (we are pursuing grant funding at this time) so you will have to order the kits on your own. We still have the in-house DNA, Bacterial Antibiotic Resistance, and Biofuel kits available. When filling out the form below, please give us a few date choices to allow for flexible scheduling since availability of the equipment can be affected by delays in equipment returns. Our new web site is: www.ATCBiotech.org. We look forward to working with you. Date Request Received Mandy Date Equipment Sent Contact information: Date Equipment Returned # of students/class PCR Machine (circle) has Amanda Latimer; MS Biotechnology Instructional Coordinator Athens Technical College 800 U.S. Hwy 29 N Athens, GA 30601-1500 alatimer@athenstech.edu need Your Information: In order for me to process your equipment/kit requests I need the following information. Also, our grants require us to maintain information of our impact on students and teachers, so please fill in class type and # of students in each class that are going to be using our kits/equipment. Thank you Name: ________________________ email address: ______________________ School name: ___________________________________________________________ School address: _________________________________________________________ School phone: __________________________________________________________ Will other teachers be using this kit with you? If so: Name: ________________________ Name: ________________________ email address: ______________________ email address: ______________________ 1 n/a Name: ________________________ Name: ________________________ email address: ______________________ email address: ______________________ Students and classes equipment will be used in: Number of students/class: __________ Course ____________________Teacher___________ Number of students/class: __________ Course ____________________ Teacher___________ Number of students/class: __________ Course ____________________ Teacher___________ Number of students/class: __________ Course ____________________ Teacher___________ Number of students/class: __________ Course ____________________ Teacher___________ We recommend the following Bio Rad kits to start with: (To order the Bio Rad kits go to: www.explorer.bio-rad.com or contact Andrew_Greer@bio-rad.com (Georgia Bio Rad Rep)) Please check which kits you will be using and approximate dates for lab. Note: All kits serve 8 stations of 2-4 students-(the ELISA 12 stations of 2-4 students) Date ____ELISA Immuno Explorer Kit (166-2400EDU-$120.00 or refill 166-2401EDU-$86.78) ___________ ____pGLO Bact.Transform. Kit (166-0003EDU-$89.00 or refill 166-0555EDU- $51.68) _____________ ____Rest. Digest & Analysis of Lambda DNA Kit (166-0002EDU-$99.00) _______________ or refill (166-0012EDU-$77.03) ____Biofuel Enzyme kit (166-5035EDU- $125.00) _______ Other _______________________________________ _______ Other _______________________________________ _______________ _______________ _______________ Bio Rad Advanced Kits-(require prior training to do PCR) (To order the Bio Rad kits go to: www.explorer.bio-rad.com or contact Andrew_Greer@bio-rad.com (Georgia Bio Rad Rep)) Note: All kits serve 8 stations of 2-4 students. _____PV92 PCR Informatics Kit (DNA from hair or cheek cells) ______________ (166-2100EDU, $199.00 or refill 166-2119EDU, $97.00 + need protease solution 166-2003EDU, $33.00) _____GMO Investigator Kit (166-2500EDU, $175.00 or refill 166-2501EDU, $118.00) ___________ _____Comparative Proteomics Kit (166-2700EDU $165.00 refill 166-2701EDU $69.00) ____________ Note: you will also need to order Mini Protean TGX pre-cast polyacrylamide gels 456-1093EDU; $74.40 (10 gels). OASIS In-house kits: These kits are free-(Note: Kits are assembled based on # of students you report / lab). 2 _______Micro-pipetting Exercise _______Cheek Cell DNA kit _______Antibiotic Resistance _______ Biofuel Lab # students_________ # students_________ # students_________ # students_________ _______________ _______________ _______________ _______________ Equipment needs for each lab: (Note: Please place an X by equipment that you need. OASIS will determine your exact equipment #s (ex: 2 vs. 4 gel rigs) based on the largest number of students you reported for classes listed above.) If you already have equipment but need an extra set of pipets, etc., just indicate # needed. ELISA Immuno Explorer Kit: ___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) ___ Pipet tips 1000 µl & 200 µl (box of 96 ea) pGLO Bacterial Transformation Kit: ___ UV light ___ 37ºC incubator oven ___ Water bath ___ Pipet tips 1000 µl & 200 µl (box of 96 ea) ___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) Restriction Digest & Analysis of Lambda DNA Kit: ___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) ____ Gel rigs (complete with comb and gel tray) ___ Power supply ___ Pipet tips 1000 µl & 200 µl (box of 96 ea) ___ Micro centrifuge ___ Water bath ___ Light Box Comparative proteomics kit (fish muscle protein kit): ___ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) ____ Pipet tips 1000 µl & 200 µl (box of 96 ea) ____ Power supply ____ Water bath ____ Gel rigs (complete with comb and gel tray) or ____ Protein polyacrylamide rigs (need pre-cast gels) ____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) ____ Low melt agarose 3 ____ ____ Acetic acid 100 ml Alcohol/ethanol 400 ml Biofuel Enzyme kit: ____ Spectrophotometer _____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) _____ Lab tape _____ Mortar & pestles _____ Water bath _____ Incubation oven _____ Mini centrifuge _____ Pipet tips 1000 µl & 200 µl (box of 96 ea) The following kits require prior experience with PCR: PV92 PCR Informatics kit: ___ Gel rigs (complete with comb and gel tray) ____ Pipet tips 1000 µl & 200 µl (box of 96 ea) ____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) ____ Power supply ____ Thermal cycler ____ Micro centrifuges ____ Water bath ____ Pipet tips with aerosol barriers ____ Light box GMO Investigator kit: ____ Gel rigs (complete with comb and gel tray) _____ Set of adjustable volume pipets (each set =p20ul, p200ul & p1000ul) _____ Pipet tips with aerosol barriers _____ Thermal cycler _____ Power supply _____ Mortar pestle _____ Light box _____ Micro centrifuge OASIS In- House produced kits: Micro-pipetting exercise kit: ___ Adjustable volume micro-pipets (P20, P200 & P1000) ____ 96 well plates ____ 15 ml tubes of colored water- 1 each of blue, green, yellow & red. 4 Cheek Cell DNA kit: ____ Transfer pipettes ____ 15 ml tubes with water ____ DNA extraction buffer ____ 90% ETOH ____ Paper towels ____ Macro Pipettor Antibiotic Resistance kit ____ 70% ETOH ____ Agar ____ Petri plates / 1 per student ____ Antibiotic discs ____ Sterile cotton swabs ____ Gloves ____ Broth tubes, 1 per student. ____ Agar slant bacterial cultures, 1 per 4 students. ____ Biohazard bag Biofuel Lab ___ Heated stir plates and stir bars. ____ 250 ml separatory flask and support ring. Other equipment available: ___ pH meters ___ TI 83 graphing calculator (2 - available) ____ Portable balances max 1500 g (2 - available) ____ Heating stir plates (14 available) ____ Spectrophotometer (1 - available) ____ Portable balances (2 - available) ____ Vernier labPro- (6 - available) ____ Vernier temp probes (6 - available) ____ Lab timers (8 - available) 5