Interleukin (IL)-11, human

Interleukin (IL)-11, human
IL-11 is a thrombopoietic growth factor that directly stimulates the proliferation of
hematopoietic stem cells and megakaryocyte progenitor cells and induces
megakaryocyte maturation resulting in increased platelet production. IL-11 is a
multifunctional cytokine that has pleiotropic effects on a variety of tissue including the
bone marrow and intestinal mucosa. IL-11 also stimulates proliferation of primitive
stem cells and multipotential and committed progenitor cells from various sources
including cord blood, bone marrow, and peripheral blood in different culture systems
and increases the commitment of primitive stem cells into the progenitor cell
compartment. IL-11 sustains hematopoiesis resulting in an increased number of
megakaryocytes and erythropoiesis, lymphopoiesis, and myelopoiesis. IL-11 acts
synergistically with other early and late acting growth factors that stimulate various
stages and lineages of hematopoiesis.
Cat. No.: Z00375
Size: 1 mg or 50 ug or 10 ug
Source: P. Pichia.
Endotoxin Level: Less than 2
ng/mg (2 IEU/μg) determined
by LAL test.
MW: 19.2 KD+/-10%
determined by reduced
Version: 03/21/2007
UV Scan
The maximal absorption wave is 278+/-3 nm.
Greater than 95% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Residual DNA: less than 2 ng/mg analysis by solid phase blot.
Residual host cell protein: less than 0.1% analysis by ELISA.
Specific Activity
The specific activity as determined was found to be greater than 8.0×10 6 IU/mg.
Lyophilized rHuIL-11 although stable at room temperature for 3 weeks, should be stored
desiccated below -18°C. Upon reconstitution rHuIL-11 should be stored at 4°C
between 2-7 days and for future use below -18°C. For long term storage it is
recommended to add a carrier protein (0.1% HSA or BSA). Please prevent
freeze-thaw cycles.
The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives.
It is recommended to reconstitute the lyophilized rHuIL-11 in sterile 18 MΩ-cm H2O not
less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Dimers and aggregates
Residual DNA: less than 2 ng/mg analysis by solid phase blot.
Residual host cell protein: less than 0.1% analysis by ELISA.
Sequence analysis
The sequence of the first fifteen N-terminal amino acids was determined and was found
to be Gly-Pro-Pro-Pro-Gly-Pro-Pro-Arg-Val-Ser-Pro-Asp-Pro-Arg-Ala.
** For non-clinical research use only. **
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