Lesson Plan Summary Sheet
Teacher:
Howerton
Subject:
Biotechnology 2
Topics:
Unit B3: Proteins
Date
10/27
Essential Question(s)
How does the structure of proteins relate to its
function?
Activities
□ View Proteins as Products
Powerpoint
□ Activity 5.1 Students will create
informational posters about a given
protein’s structure and function
□ Lab11: The Action of Different
Enzymes on Apple Juice Production
Vocabulary
Primary
Secondary
Tertiary
Quaternary
Learning Logs
B31: Write a job description for the
protein of your choice. Describe its
structure and function.
10/28
What role do enzymes play in the cell?
Enzyme
Substrate
Lock-and-key
cellulase, pectinase,
protease, rennin
Kinetics
Titrations
B32:Explain what enzymes are and
the factors that affect them.
10/29-30
What enzyme kinetics affect enzyme activity?
□ View Enzymes powerpoint
□ Lab 12: Enzyme Catalysis
11/2
How Do You Know When You Have Some
Amylase?
□ Lab14: Assaying for Amylase
Activity
Assay
B33:Complete Lab13 : Developing an
Assay for Protein Activity (Amylase)
for homework
B34:Explain the function of amylase.
11/3
Do any bacterial species have native amylase
activity?
□ Lab 15: Searching for Native
Amylase Producing Bacteria
Halos
Native
B35:Explain how you can test for
amylase in bacteria.
11/4
How do you use the spectrophotometer to
study molecules?
Absorbance, OD,
transmittance
11/5
How can you harvest amylase produced by
bacteria?
□ Lab 16: Using the
Spectrophotometer to Study the
Amylase Protein
□ Lab 17: Harvesting Amylase from
Bacterial Cultures
11/6
How can you change the buffer that a protein
is in?
□ Lab 18: Dialysis of Proteins into
Different Buffers
Dialysis
B36:Explain why it is important to
find the lambda max for a proteinreagent mixture?
B37:Explain at what time during the
growth of a bacterial culture is it best
to harvest a protein?
B38:Explain how dialysis works.
11/9
How do you use chromatography to separate
proteins in a solution?
□ Lab 19: Using Ion-Exchange to
Purify Amylase from Broth
Ion exchange
chromatography
Affinity
chromatography
Scale-up
broth
B39:Explain ion exchange
chromatography
□ Lab 20: Testing for the Presence of
Protein in Solution
□ Lab 21 Identifying Amylase using
SDS PAGE
Standard curve
11/12
How do you determine the amount of protein
in a sample?
How do you characterize proteins by PAGE?
11/13
What protein products are in the line?
□ Activity 8.4: Product Pipeline Study
varies
11/16
What techniques are used to manipulate
proteins in a biotech lab?
□ Review and wrap-up
□ Protein Project Due
none
11/17
What techniques are used to manipulate
proteins in a biotech lab?
□ Test on Unit
□ Vocabulary and Learning Logs
Due
none
11/10
SDS, polyacrylamide
Laemmli Buffer
Denaturation
B310:Explain how the Bradford assay
works.
B311: You run a gel from an ionexchange chromatography. You see
6 bands in the wash samples and 3
bands in the elution sample,
including a band of 61kD. Has
amylase been purified from the other
proteins in the broth? What else can
you do?
B312:Report on a protein product of
interest.
B313:Explain how you plan to
prepare for the test and whether you
were successful.
B314:Write a reflection on your
effort and what you have learned this
unit.