Section: Genetically modified animal or plant generation and
production
Note: If you will only purchase or transfer transgenic plants or animals from a vendor or
collaborator, do not fill out this page.
If your project involves generation of genetically modified animal (by pronuclear injection and
implantation) with downstream phenotype evaluation, novel crosses involving transgenic parents in
unrelated lines, or congenic production with at least one transgenic parent, please answer the
following. The response to each question should require no more than five sentences.
1. Research project summary. Describe your project objectives and experimental design and
describe the known or hypothesized function of the gene(s) of interest. Describe your goals in
working with transgenic lines, developing targeted gene knockouts, or breeding to produce
congenic lines. Specify the animal strain(s) to be used for transgenic production, crosses, or
congenic development. If you use UCSF core facility to produce the transgenic animals, identify the
facility.
2. Risk assessment. Describe the potential consequences of introducing your transgenic animal
genome into wild populations, or inadvertently into established laboratory colonies. Identify lethal
combinations (if known) that can result from your breeding plan.
3. Risk minimization. Specify your measures to ensure that your transgenic animals do not
escape containment. Discuss precautions taken during routine colony maintenance and animal
transport within and between structures. Please describe your record-keeping method for tracking
transgenic animals, and your methods for containment and carcass disposal (per Appendices G
and Q of the NIH Guidelines).
Plants. If your project involves recombinant plant development and downstream phenotype
evaluation, please describe your project objectives, experimental design, risk assessment, and risk
minimization. Please describe your record-keeping method for tracking transgenic plants and your
methods for containment and disposal (per Appendix P of the NIH Guidelines).
On title page
New box-- Genetically modified animal or plant generation and
production
Description:
This includes experiments that involve genetically modified animal generation (by pronuclear
injection and implantation) with downstream phenotype evaluation, novel crosses involving
transgenic parents in unrelated lines, or congenic production with at least one transgenic parent.
OR
This includes experiments that involve recombinant plant development and downstream phenotype
evaluation.