Autoclave Protocols
Autoclave Room: Galvin 043
For Solids: use for tips, glassware, etc.
1) For tips, fill open boxes with new tips (bagged or racked). Once full, close box
and put autoclave tape on box.
2) For open glassware, place aluminum foil over top. Place autoclave tape over foil
and make sure it sticks well.
3) Place tips/glassware in autoclave tray.
4) Place tray in autoclave.
5) Close door tightly by using turn-wheel. Door should be as tight as possible to
avoid steam leaks.
6) Following buttons and their associated programs are below:
Button
1
2
3
4
Program
Gravity
Liquid
Gravity
Liquid
Sterilization Time
15min
20min
25min
40min
Dry Time
10min
N/A
25min
N/A
Total Time*
45
45
1 hour
1 hour
*Total time includes wait time to allow autoclave to cool before taking items out.
7) Press button once to see program, and then again to start program.
a. Program 1 ideal for pipet tips, although some may not dry completely.
Use Program 3 for longer dry time if needed.
b. Program 2 is ideal for any liquids, unless specifically told to use Program
4.
8) Slowly open autoclave door, allowing steam to escape. Temperature will be
between 150-200F, so be careful when opening.
9) Take tray out carefully, and be sure to close door and seal for the next person.
10) Allow for all items to cool/dry for at least 2 hours before using.
a. Place autoclaved tip boxes in 70C oven to dry.
b. Allow tips to dry at least overnight before using.
c. Keep all autoclaved glassware covered until you use it.
For Liquids:
1) For glassware with lids, be sure to use total volume of at least 2X more than what
you are autoclaving to avoid spillage once boiling starts. i.e. use a 1L bottle to
autoclave 500mL water.
2) Place lid/screw cap on loosely in order to keep pressure from building up inside
bottle. Place autoclave tape on lid.
a. If liquid is placed in a conical flask with an opening, place aluminum foil
over opening, making sure that it is covered but loose and place tape on
aluminum foil.
3) Place in autoclave tray.
4) Follow autoclaving procedure for Solids (above), using liquid programs (2 or 4),
instead of solid programs (1 or 3).
5) Remove in same way as above. Allow to cool before using.