Policy #MI\BA\v21
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Issued by: Laboratory Manager
Approved by: Laboratory Director
Page 1 of 18
Original Date: October 08, 2002
Revision Date: January 25, 2015
Annual Review June 12, 2014
BACTERIA and YEAST WORK-UP
TABLE OF CONTENTS
Vitek MS Guide to Bacteria and Yeast Identification per Bench: .................................................. 2
Reference Bacteria and Yeast Identification Tables and Flowcharts ............................................. 3
AEROBIC BACTERIA .................................................................................................................. 3
GRAM POSITIVE BACTERIA ................................................................................................. 3
GRAM POSITIVE COCCI - Catalase-Positive ..................................................................... 3
GRAM POSITIVE COCCI – Catalase-Negative ................................................................... 4
Identification tests for Group B streptococcus ............................................................... 5
GRAM POSITIVE BACILLI ................................................................................................. 6
GRAM NEGATIVE BACTERIA .............................................................................................. 8
GRAM NEGATIVE COCCI / DIPLOCOCCI ....................................................................... 8
Identification of Neisseria gonorrhoeae ......................................................................... 8
Identification of Neisseria meningitidis .......................................................................... 9
GRAM NEGATIVE BACILLI - Oxidase-Negative, Fermenter .......................................... 10
GRAM NEGATIVE BACILLI – Oxidase-Negative, Non-Fermenter ................................. 10
GRAM NEGATIVE BACILLI – Oxidase-Negative or Weak Positive ?Haemophilus
species ................................................................................................................................... 10
H.haemolyticus2 identified from Vitek-MS .................................................................. 10
H.influenzae .................................................................................................................. 10
GRAM NEGATIVE BACILLI – Oxidase-Positive ............................................................. 11
Identification of H. pylori ............................................................................................. 11
Urine Pathogens Workup .............................................................................................................. 12
Enteric Pathogens Workup ........................................................................................................... 14
ANAEROBIC BACTERIA .......................................................................................................... 15
YEAST IDENTIFICATION ......................................................................................................... 16
REFERENCES ............................................................................................................................. 17
Record of Edited Revisions .......................................................................................................... 18
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Policy #MI\BA\v21
Page 2 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Bench
Blood
Culture
Resp
Vitek MS Guide to Bacteria and Yeast Identification per Bench:
Colif GNB
Alpha
GNDC
Dipth
Staph BHS** Entero strep
LF
NLF
MS
MS
Significant
MS
MS
Non-
SS
MS
Significant
Misc.
Sterile
Nonsterile,
not
Seminal/
Gyne
swabs
Anaerobes
Bacillus
Yeast
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
Gram
MS
MS
MS
---
Bile
solub.
---
---
---
---
---
---
---
MS to
R/O
Crypto
MS
MS
MS
MS
MS
MS
MS
MS
MS
Gram
MS
MS
MS
SS
MS
BE or
PYR
---
---
---
Ox.
---
MS
---
---
---
--
BE/
Prolex
BE
---
---
---
---
---
----
---
---
---
MS
MS
---
---
significant
Gynae.
GBS
Haemophilus
MS
Prolex
MS
MS
MS
MS
MS
MS
MS
Gram
MS
---
MS
on all
NLF*
NSF*
and
green*
---
---
---
Significant
growth
Enteric
* use
Oxoid
Shigella
Latex aggn
on all Ecoli
NLF,NSF
Urine
---
---
---
---
---
MS/
Ox/
Gram
MS
(except: E.coli MS
MS
MS
N/A
N/A
MS
ID from Pink
colony)
*see VTMS MALDI-TOF Manual for acceptance rules
VITEK-MS-V2-speciesList Created-Translated list
** MS for all BH colonies. Use Prolex as applicable to workflow and for large BH colony types
See also Reference Bacteria and Yeast Identification Tables and Flowcharts for other tests
MS/
Prolex
MS
PROCEDURE MANUAL
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MS
Policy #MI\BA\v21
Page 3 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Reference Bacteria and Yeast Identification Tables and Flowcharts
AEROBIC BACTERIA
GRAM POSITIVE BACTERIA
GRAM POSITIVE COCCI - Catalase-Positive
Tests
T-DNase1
Staph-Slide
Agglutination1,2,4
Tube
coagulase1,4
Novobiocin
susceptibility3
GenProbe5
Oxidase
Growth in 6.5%
NaCl
Bacitracin
PYR8
LAP
Ornithine
decarboxylase
S. aureus
CNST
S. saprophyticus
S. lugdunensis
Micrococcus
species6
Stomatococcus
species7
+
+
-
-
-
-
-
+
-
-
-
-
-
N/A
S
R
S
N/A
N/A
>1,500
PLU
+
<1,200
PLU
+
N/A
N/A
N/A
N/A
+
+
+
+
-
N/A
N/A
N/A
N/A
V
N/A
-
N/A
N/A
N/A
N/A
+
N/A
+
S
N/A
N/A
N/A
N/A
+
+
N/A
1 compulsory test for Blood Cultures
2 compulsory test for all specimens other than Blood Cultures
3 test on urines from female of childbearing age
4 compulsory test for Infection Control MRSA screens
5 confirmatory test if needed
6 obligately aerobic
7 catalase variable
8 compulsory test for CNST isolated from Blood Cultures and sterile sites; set up ornithine decarboxylase if PYR is
positive to rule out S. lugdunensis
Interpretation:
PYR
Ornithine
Report
CNST
+
+
S. lugdunensis
Indeterminant
CNST
Indeterminant
+
Send to PHOL for ID
PROCEDURE MANUAL
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Policy #MI\BA\v21
Page 4 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
GRAM POSITIVE COCCI – Catalase-Negative
1.
beta-hemolytic large colonies on Blood Agar:
Test
Gram Stain
Catalase
BE
Streptococcus Latex Agglutination
2.
Group A, B, C or G streptococcus
g+c
Agglutination with Group A, B, C or G
beta-hemolytic small colonies on Blood Agar:
Test
Gram Stain
Catalase
BE
Streptococcus Latex Agglutination
Streptococcus anginosus group
g+c
-/weak
Agglutination with Group A, C, F, G or
non-groupable
+
VP
Non-hemolytic or -hemolytic on Blood Agar:
3.
Streptococcus
pneumoniae
viridans
Streptococci
Aerococcus
urinae
Enterococci1,4
Leuconostoc/
Pediococcus
Others
Gram Stain
g+dc
g+c
g+c
g+c
g+c
Catalase
BE
Vancomycin
Bile
Solubility
Optochin
PYR
LAP
Vitek id-gp
card
S
+
S
-
g+c
clusters,
tetrads
-
+1
S / R3
N/A
R
N/A
V
S
N/A
S
N/A
N/A
ID if
questionable
identification
by Bile
solubility
and/or
Optochin
R
-
N/A
+
N/A
-
N/A
N/A
ID2
Not in data
base, set up
API Strep
strip
ID – will ID
Gemella,
Strep.
anginosus
group5, nonhaemolytic
group B strep
Test
Some ID;
report as
viridans
Strep group
R
+
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 5 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
1.
For urine, wounds and superficial specimens, report as Enterococcus species based on Bile
Esculin result (do not need to further speciate).
2.
If Vitek ID as E. faecium and Amp-S, or other Enterococcus other than E. faecalis, set up
sugars: Xylose, Arabinose, MGP, PYR
3.
Enterococci species that are I or R to Vancomycin must be checked for yellow pigment. For
isolates negative for pigment, set up rapid xylose test. Positive xylose (within 2 hours)
isolates are E. gallinarum. For non-yellow pigmented isolates and negative rapid xylose
isolates, set up Enterococcus MIC panel. Unidentified isolates are to be sent to the Provincial
Health Lab for identification.
4.
Enterococcus identification:
Test
Enterococcus
faecalis
BE
+
Yellow pigment
Pyruvate
+
Ampicillin
S
5.
Enterococcus
faecium
+
R
Enterococcus
gallinarum
+
S
Enterococcus
casseliflavus
+
+
S
Streptococcus anginosus group – VP+
Identification tests for Group B streptococcus
Hemolysis
β
Catalase
+
discard
α or γ
Catalase
Strep
grouping
+
discard
Bile esculin (1 hour)
+
discard
Strep grouping
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 6 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
GRAM POSITIVE BACILLI
Test
Catalase
BE
Penicillin
Resistance
Motility
Urease
Vancomycin
Listeria
Arcanobacterium
Erysipelothrix
+
+
N/A
N/A
N/A
+
-
-
N/A
Set up
Vitek idgp card
and
rhamnose
C. jeikeium
C.
(JK)
urealyticum
+
+
Yes
N/A
-
N/A
N/A
Set up Vitek id-gp card
N/A
Set up
API
CORYNE
+
N/A
Set up
API
CORYNE
Lactobacilli
V
N/A
N/A
R/S
Set up
RapID ANA
Note:
 Catalase positive, Penicillin sensitive, Gram positive bacilli that are not Listeria or Bacillus
species, report as “Corynebacterium species”; except in urine (rule out C. urealyticum).

Catalase positive, Penicillin resistant, Gram positive bacilli that do not identify as
Corynebacterium jeikeium, report as “Corynebacterium species”; except in urine (rule out C.
urealyticum).

For aerobic pigmented, Catalase positive, Gram positive bacilli, send isolate to PHOL for ID.

Aerobic spore-forming bacilli:
Test
Bacillus cereus
Bacillus anthracis
Haemolysis on Blood Agar
Lecithinase by Egg Yolk Agar
+
Opaque zone
around colonies
+
No
Opaque zone
around colonies
Yes**
Moltility by Motility medium
PHOL for confirmation
Bacillus species other than
B. cereus or B. anthracis
+/No opaque zone
+/No
**Send isolate to PHOL. Package the isolate in a SAFT PAK container, labeled “Bacillus
species non-motile” and shipped as dangerous goods. Phone PHOL at 416-235-5706 to inform of
bacillus species to rule out B anthracis.
 For faintly staining beaded gram positive bacilli, perform a modified Kinyoun (rule out
Nocardia) and Kinyoun (rule out Mycobacterium)
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 7 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up

All gram positive bacilli isolated from tissues and sterile sites, perform a modified Kinyoun
(rule out Nocardia) and Kinyoun (rule out Mycobacterium)
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Page 8 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
GRAM NEGATIVE BACTERIA
GRAM NEGATIVE COCCI / DIPLOCOCCI
Test
Oxidase
Catalase
Tributyrin
API NH
Phadebact
M. catarrhalis
+
+
+
N/A
N/A
N. gonorrhoeae
+
+
N/A
ID
ID
N. meningitidis
+
+
N/A
ID
N/A
N. species
+
+
N/A
ID
N/A
Identification of Neisseria gonorrhoeae
Oxidase
Negative
(not Neisseria)
Positive
Gram stain  gnb (not Neisseria)
Gram negative diplococcus
API NH
Phadebact
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 9 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Identification of Neisseria meningitidis
Oxidase
Negative
(not Neisseria)
Positive
Gram stain  gnb (not Neisseria)
Gram negative diplococcus
Vitek2 NH
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Page 10 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
GRAM NEGATIVE BACILLI - Oxidase-Negative, Fermenter
Test
MUG*
INDOLE*
Vitek id-gn
api20E
E. coli
+
+
ID
Use when not ID by id-gn
Not E. coli
+/ID
Use when not ID by id-gn
* Use for urine isolates
Note:
Unidentified isolates are to be sent to the Provincial Health Lab for identification.
GRAM NEGATIVE BACILLI – Oxidase-Negative, Non-Fermenter
Test
Vitek id-gn
api20E
api20NE
Some ID
Some ID
Most ID
Note:
Unidentified isolates are to be sent to the Provincial Health Lab for identification.
GRAM NEGATIVE BACILLI – Oxidase-Negative or Weak Positive ?Haemophilus species
Test
Gram
Satellitism
ALA
Catalase
H. influenzae1
g-cb
+
+
H. parainfluenzae
g-cb
+
+
-
H. haemolyticus2
g-cb
+
+
Note:
H.haemolyticus2 identified from Vitek-MS
If an isolate is identified from the Vitek-MS ID is H.haemolyticus, send the isolate to PHOL for
confirmation and specify "rule out H.influenzae" on PHOL requisition.
H.influenzae1 identified from Blood and sterile site specimens:
Send isolate to the Provincial Health Lab for Serogroup b typing.
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 11 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
GRAM NEGATIVE BACILLI – Oxidase-Positive
Test
Cetrimide*
Vitek id-gn
api20E
api20NE
Growth at 42oC
Ps. aeruginosa
+
ID
ID
ID including mucoid strains
+
Not Ps. aeruginosa
Some ID
Some ID
Most ID
+/-
* Growth AND green pigment
Note:
Unidentified isolates are to be sent to the Provincial Health Lab for identification.
Identification of H. pylori:
Test
H. pylori
Gram stain
Catalase
Oxidase
Urea slant (rapid)
Cephalothin 30g
Nalidixic acid 30g
Small, gram negative gull-shaped or spiral
+
+
+
S (inhibition)
R (no zone)
PROCEDURE MANUAL
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Page 12 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Urine Pathogens Workup
Suspect Organism
E.coli (Lactose
fermenter)
Enterobacteriaceae
Pseudomonas
aeruginosa
Non-fermenters
Tests to be performed and expected result
Oxidase: Negative
MUG: Positive + Indole: Positive
Oxidase: Negative
Oxidase: Positive
Characteristic appearance + Cetrimide: Positive
Oxidase: Negative/Positive
Yeast
 If isolated from aseptically collected urine:
Wet mount
Germ tube-positive : Report as C. albicans
-negative : Identify to species
Group B
streptococcus
Staphylococcus
species: aureus
S. saprophyticus
CNST
Vitek ID + Susceptibility
Vitek Susceptibility
Vitek ID + Susceptibility or
API NE + KB Sens if
applicable
Refer to Mycology
 If isolated from Voided Urine: Wet mount
Do not identify. Report as yeast.
Strep. Latex Agglutination: Group B Positive
Bile esculin: Negative
Staph. Latex Agglutination: Positive
Vitek Susceptibility
Oxacillin screen
Vancomycin screen
Staph. Latex Agglutination: Negative
Novobiocin: Resistant (set up on patients 12 60 yrs and females only)
Novobiocin: Sensitive
Staph. Latex Agglutination: Negative
Enterococcus species Bile esculin: Positive
Corynebacterium
species
Identification/Susceptibility
Vitek Susceptibility
Vitek Susceptibility
Vancomycin screen
Non-haemolytic colonies on Blood Agar
Gram positive bacilli, small, palisade
arrangement.
Catalase: Positive
Urea: Negative
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UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Page 13 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Suspect Organism
Corynebacterium
urealyticum
Tests to be performed and expected result
Non-haemolytic colonies on Blood Agar
Gram positive bacilli, small, palisade
arrangement.
Catalase: Positive
Urea (rapid): Positive
viridans streptococcus Alpha-haemolytic colonies on Blood Agar
Gram positive cocci in chains
Catalase: Negative
Aerococcus urinae
Alpha-haemolytic colonies on Blood Agar
Gram positive cocci in tetrads or clusters
Catalase: Negative
LAP: Positive + PYR: Negative
Lactobacillus species Alpha-haemolytic or non-haemolytic colonies
on Blood Agar
Gram positive thin bacilli
Catalase: Negative
Bacillus species
Gram positive large bacilli with square ends
Motility: Positive
Identification/Susceptibility
API CORYNE for ID
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Policy #MI\BA\v21
Page 14 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Enteric Pathogens Workup
Organism
TSI
ONPG
PPA
Motility
Indole
Urea
S. typhi
-/+1
-
-
+
-
-
S. arizonae
d/+ H2S
+
-
+
-
-
S. paratyphi A
-/+2
-
-
+
-
-
Other Salmonella
-/+ H2S
-
-
+
-
-
S. sonnei
-/+
+
-
-
-
-
S. dysenteriae
-/+
d
-
-
d
-
S. flexneri (1-5)
-/+
-
-
-
d
-
S. flexneri (type 6)
-/+4
-
-
-
d
-
S. boydii
-/+
-
-
-
d
-
Y. enterocolitica
d/+
+
-
-3
d
+5
1
may produce small amounts of gas and /or H2S
occasionally produces H2S weakly
3
non-motile at 35oC; motile at room temperature (read motility within 15 minutes after
removal from incubator.
4
may produce a small amount of gas
5
after overnight incubation
2
“d” indicates variable results
E.coli O157
1. Oxidase test negative
2. MUG test negative
3. 0157 latex agglutination test positive
4. Send the isolate to PHOL for confirmation and H typing.
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Page 15 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
ANAEROBIC BACTERIA
Test
Peptostreptococcus
species
Propionibacterium
species
Gram
g+c
N/A
No growth
g+b small,
branching
+
No growth
No growth
Catalase
Subculture BA
CO2
Subculture
CHOC CO2
Subculture
BRUC AnO2
BBE
RapID ANA**
*
**
Anaerobic
non-spore
forming
Gram
positive
bacilli
g+b small
Clostridium
species
B. fragilis
Anaerobic
Gram
negative
bacilli
g+b, large
g-b
g-b
No growth
N/A
No growth*
N/A
No growth
N/A
No growth
No growth
No growth
No growth*
No growth
No growth
Growth
Growth
Growth
Growth
Growth
Growth
N/A
N/A
N/A
N/A
N/A
N/A
Some ID
+
N/A
ID
Some Clostridium species can grow aerobically
Usually not done – report as anaerobic gram positive or gram negative bacilli

Anaerobic, small Gram positive bacilli resembling diphtheroids that are catalase positive
should be reported as “Propionibacterium species”.

Anaerobic, small Gram positive bacilli that are catalase negative should be reported as
“Anaerobic non-spore forming Gram positive bacilli”.
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
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Page 16 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
YEAST IDENTIFICATION
Identify yeast as per site of isolation:
1)
Sterile sites and biopsy specimens:
a) Germ tube: Positive - Report as "Candida albicans" “isolated”.
b) Germ tube: Negative - Report as “Yeast” “isolated” “identification to follow”
and send the isolate to Mycology for identification.
2)
Respiratory sites isolates:
Significant growth – For sputum (>2+ growth and predominant OR 1+ growth and
predominant and if pus cells are seen on gram stain) OR for BAL specimen (amount
greater than that of commensal flora):
a) Germ tube: Positive - Report as "Candida albicans"
b) Germ tube: Negative - Rule out Cryptococcus using Urease test. If Urease is
negative, report as "Yeast, not Candida albicans or Cryptococcus". If
Urease is positive, confirm purity, subculture isolate onto a SAB
plate and send the SAB and original plate to Mycology for further
identification ASAP.
Insignificant growth – i.e. any amount of yeast other than what has defined as
significant growth.
Rule out Cryptococcus using Urease test. If Urease is negative, report as part of
Commensal flora without specifically mentioning the presence of yeast. If Urease
is positive, confirm purity, subculture isolate onto a SAB plate and send the SAB
and original plate to Mycology for further identification ASAP.
3)
Voided urines, superficial sites, wounds and drainage fluids:
No Germ tube performed. Report as "Yeast” with quantitation.
4)
Isolates from all other sites:
a)
Germ tube: Positive - Report as "Candida albicans".
b)
Germ tube: Negative - Report as "Yeast, not Candida albicans".
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY" are not
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D:\116106458.doc
Policy #MI\BA\v21
Page 17 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
REFERENCES
H.D. Izenberg. 2003. Guildlines for Identification of Aerobic Bacteria, 3.16.1 in Clinical
Microbiology Procedures Handbook, 2nd ed. Vol.1 ASM Press, Washington, D.C.
H.D. Izenberg. 2003. Schemes for Identification of aerobic Bacteria, 3.18.1.1 – 3.18.2.1 in
Clinical Microbiology Procedures Handbook, 2nd ed. Vol.1 ASM Press, Washington, D.C.
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY" are not
controlled and should be checked against the document (titled as above) on the server prior to use
D:\116106458.doc
Policy #MI\BA\v21
Page 18 of 18
Department of Microbiology
Laboratory Policy & Procedure Manual
Section: Bacteria and Yeast Work-up
Record of Edited Revisions
Manual Section Name: Bacteria and Yeast Work-up Manual
Page Number / Item
Annual Review
Annual Review
Annual Review
Yeast ID moved from Respiratory and Wounds Manuals
Identification of Neiserria gonorrhoeae added
Identification of Neiserria meningitidis added
Identification of H. pylori moved from Respiratory
Manual
Identification for S. lugduensis in blood and sterile sites
Annual Review
Annual Review
Annual Review
Change reporting - If motile, report as “Bacillus sp. not
B. antracis.”
Added Kinyoun and Modified Kinyoun for gram positive
bacilli workup to rule out Norcardia and Mycobacterium
Annual Review
Annual Review
Added shipping information for “Bacillus “ to PHOL
Annual review
Changed S. anginosis BE reaction from – to -/weak
Added Bacillus ID table
Annual review
Added Bacteria and Yeast ID Bench workflow to
accommodate MS ID
Annual Review
Updated workflow for Neiserria gonorrhoeae
Updated workflow for Enterococcus
Annual Review
H. haemolyticus ID by Vitek-MS workup
Urine ID by MS and E.coli ID by pink colonies
Enteric Pathogen Workup moved from Enteric Manual
Date of Revision
May 12, 2003
May 26, 2004
May 12, 2005
April 6, 2005
April 6, 2005
April 6, 2005
April 6, 2005
Signature of
Approval
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Feb 14, 2006
July 12, 2006
August 13, 2007
October 9, 2008
March 04, 2009
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
June 3, 2009
Dr. T. Mazzulli
October 10, 2009
October 10, 2010
January 11, 2011
May 31, 2011
July 10, 2012
July 10, 2012
July 10, 2012
November 04, 2013
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
November 04, 2013
April 17, 2014
April 24, 2014
June 12, 2014
January 25, 2015
January 25, 2015
January 25, 2015
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
Dr. T. Mazzulli
PROCEDURE MANUAL
UNIVERSITY HEALTH NETWORK / MOUNT SINAI HOSPITAL DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY" are not
controlled and should be checked against the document (titled as above) on the server prior to use
D:\116106458.doc