dsRNA viruses RNA / protein tables: Edited by Peter Mertens and Denis Bamford
The dsRNA genome segments and proteins of Pseudomonas phage Phi 6
(genus Cystovirus : family Cystoviridae)
last updated 11/09/2002
dsRNA
(Size, bp)
[5,8,20,23]
Open reading
frames
(ORFs)
Proteins
(': protein
structure/
function)
Size 'aa'
(kDa )
[5,8,20,23]
protein copy
number per
particle
location
Function
Self assembles with proteins P2, P4 and P7 into procapsid particles
[9, 29]. Corresponds to the T=2 particle in BTV [11]. Controls
procapsid size and organization. Binds minor proteins and RNA.
Conserved in dsRNA viruses.
Semi-conservative RNA dependent RNA polymerase [34, 35].
Isolated protein acts on ss and dsRNA templates [18, 19]. Threedimensional structure homologous to HIV reverse transcriptase and
hepatitis C polymerase [3]. Binding sites for nucleic acid, NTPs,
Mn and Mg ions known [3].
Binds to the receptor [25] , the pilus IV of Pseudomonas syringae
[30, 31]
[5,8,20,23]
L
(6,374)
Gene 1
P1
(T=2)
769
(84.986)
120
[2. 6]
inner particle shell
L
Gene 2
P2 (pol)
[3, 18, 19]
664
(74.791)
12
[6]
inner particle
M
(4,063)
Gene 3
P3 (spike)
[17]
648
(69.178)
L
Gene 4
P4 (NTPase)
[7, 10, 16]
331
(35.032)
S
(2.948)
Gene 5
P5 (mur)
[4, 12, 21]
219
(24.018)
between the membrane and NC
[12]
M
Gene 6
L
Gene 7
S
Gene 8
P6 (fus)
[1]
P7 (assembly
factor) [29]
P8 (T=13)
[2]
167
(17.229)
160
(17.169)
148
(15.873)
Membrane
[32]
inner particle
[15]
second protein layer
[2]
S
Gene 9
89
(9.482)
M
Gene 10
P9 (env)
[13, 32, 33]
P10 (lys)
[14]
42
(4.272)
72
[7]
60
[15]
600
[2, 29]
extends from virion surface [17]
and is attached to the integral
membrane protein P6 [25]
inner particle at 5 fold axes
[7]
Membrane
[32]
Membrane
[32]
Hexameric NTPase, symmetry mismatched position [7, 10, 16].
Powers the packaging of ssRNA genomic precursors and is
involved in transcription [27]. Nucleates the assembly of the
procapsid with P1 [29]
Muralytic enzyme exposed to host peptidoglycan layer after
membrane fusion during viral entry. Also used to lyse the host cell
late in infection to release the virus [4, 12, 21].
Integral membrane protein that has membrane fusion activity and
binds the receptor -binding protein, P3 [1, 25, 32].
Dimeric, stabilizes the procapsid [15]. Increases the assembly rate
of the procapsid in vitro [29]
A trimer [29] that forms the T=13 nucleocapsid shell around the
dsRNA-containing inner particle (core) [2]. During entry P8 drives
the penetration of the nucleocapsid into the cytoplasm [26, 28].
Major membrane protein, essential for membrane formation [13, 32,
33]
Needed for cell lysis [14]
dsRNA viruses RNA / protein tables: Edited by Peter Mertens and Denis Bamford
S
Gene 12
M
Gene 13
P12
(assembly)
[13. 22, 25]
P13
L
Gene 14
P14
195
(20.293)
72
(7.649)
61
(6.810)
0
0
Non-structural
[32]
Assembly factor active in viral membrane morphogenesis [13, 22,
25].
Membrane
[8]
Non- structural
[5]
Minor, nonessential membrane protein [8, 24]
Nonessential [5].
': ': To facilitate comparisons of proteins across species, genera and families, protein structure / function indicators have been added to
protein names, eg (pol) = RNA polymerase; (T=2) = capsid protein organized with icosahedral T=2 symmetry; (T=13) = capsid protein
organized with icosahedral T=13 symmetry.
Constructed by Sarah Butcher and Dennis Bamford.
Available as a webpage on: http://www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/Cystovirus.htm
If you have any queries regarding this page please contact Peter Mertens on peter.mertens@bbsrc.ac.uk.
References.
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