Sample submission guidelines: DNA sequencing can be submitted
to the Genomics Core in three formats:
1) DNA template and sequencing primer in separate tubes: Per
sequencing reaction provide 10 ul template (template @ 200
ng/ul) and 10 ul primer (primer @ 1 pmol/ul).
2) DNA template and sequencing primer premixed: Per
sequencing reaction provide 11 ul of sample containing 200 ng
of DNA per 1 kb of template and 10 pmol of primer for plasmid
templates and 35-40 ng of DNA and 5 pmol of primer for PCR
products.
3) DNA template and sequencing primer premixed in a 96-well
format: Per sequencing reaction provide 5 ul of sample
containing 100 ng of DNA per 1 kb of template and 5 pmol of
primer for plasmid templates and 10 ng of DNA per 100 bp of
template and 5 pmol of primer for PCR products.
Important Note: the amounts give above are guidelines that function
as starting points for optimizing your specific sequencing reactions.
Many clients of the Genomics Core have come up with their own
amounts and concentrations that work based on their specific
templates / DNA isolation methods / DNA quantification methods. If
you are a new customer and are uncertain what will work for you
please contact the Core manager and we will run some free test
samples.
Sample collection times and drop-off points on the CCF campus:
Samples are collected once a day between 9:00 a.m and 9:30 a.m.
For your convenience there are a total of three sample drop-off areas
on campus: at LRI one is in the small office corridor in the middle of
the west NC1 hallway and one is in the 5th floor NB hallway, opposite
the Chairman's office. For those who would like to drop off their
samples in a refrigerated spot there is a Genomics Core refrigerator
in the NN building: past conference room NC1-202 through the door
marked "Area NN". For clients without CCF access there is a
refrigerated drop-off point in the ND shipping and delivery area (small
fridge on table).