Sanger Sequencing Request Form
ABform2015-11-20
Please complete in full.
Room #:
Dept:
Name:
Tel #:
Group Leader’s Name:
Email:
Group Leader’s Signature:
Billing Information: PO Number:
MRC 
Oxford University 
Please note : External WIMM users must provide a PO number for correct billing
How much DNA should you supply?
We will use between 1l and 14l of DNA with each primer you wish to sequence with.
The volume (l) of DNA we will use depends on the size of the DNA template (kb) and its concentration (ng/l):
The larger the template, the greater the amount (ng) of DNA required.
The weaker the concentration, the greater the volume (l) of DNA required.
Please make sure your
DNA concentrations are
less than 300ng/ul
The following table indicates how much DNA (ng) is required for different sized templates. Please supply a sufficiently large volume (l) of DNA to allow
the required amount (ng) of DNA to be used; plus at least an additional 10l extra volume (l) to enable efficient pipetting.
DNA (ng) Minimum Amount Required for Each
Sample Number
DNA Template Type and Size:
Sequencing Primer:
PCR Products:
100 - 800 bp
20 ng
Please label tube lids with your
800 - 1500 bp
30 ng
initials & consecutive numbers
1500 - 2000 bp
40 ng
Template Size
2000 - 3000 bp
60 ng
Plasmids:
3 - 8 kb
300 ng
9 - 10 kb
350 ng
Complete one box only;
11 - 12 kb
400 ng
Plasmid or PCR product
13 - 14 kb
450 ng
DNA TEMPLATE DETAILS:
Template Size
Size
Sample
Conc
Primer(s)
Sample
Conc
Primer(s)
Plasmid +
PCR prod
Plasmid +
PCR prod
Number
Number
(ng/l)
Insert (kb)
uct (bp)
(ng/l)
Insert (kb)
uct (bp)
SEQUENCING WITH STOCK PRIMERS: Please tick box to select primers required:
M13 forward
M13 reverse
T7 (pGMT7)
T3
SP6
pCDM8 Forward
OX281
pRC/CMV Forward
pRC/CMV Reverse
pSC11 5’
pSC11 3’
pcDNA3 5’
FCD5
RFC
rT7
RGFPN
FGFPC
RGFPC
T7 (Bluescript)
pCDM8 reverse
TH (-40)
pcDNA3 3’
FGFPN
CUSTOM PRIMER DETAILS: Each sequencing reaction requires 1l of primer @ 3.2 pmol/l.
Primer Name:
Concentration 3.2 pmol/l
Volume Supplied (l):
Primer Name:
Concentration 3.2 pmol/l
Volume Supplied (l):

If you would like your samples/primers back, please tick this box. Please collect them from fridge 2, third shelf down, within two weeks.
Gap
Weatherall Institute of Molecular Medicine
DNA Sequencing Service
Lab Telephone: 01865 222623

John Frankland: john.frankland@imm.ox.ac.uk
Tim Rostron: timothy.rostron@imm.ox.ac.uk
http://www.imm.ox.ac.uk/sanger-dna-sequencing