Primer Dilutions for MSU ordered primers ¨C Description of how to

Primer dilutions
1. Primers are ordered from MSU
Zhou’s lab account: 613225
Lab ID: jt
Password: tiedje
2. When primers arrive they will have a label similar to:
XXXX= primer number
XX pM/l : 
= number
3. Label tubes
4. Add 10 times the number () of milli-Q H2O to the tube to obtain a ~100
pmol/l concentration. These are the stock tubes. Store at -20C . We
routinely use 5pmol/l of primer/25l rxn