Primer dilutions 1. Primers are ordered from MSU Address: http://struct-fac.bch.msu.edu/msf/mark Zhou’s lab account: 613225 Lab ID: jt Password: tiedje 2. When primers arrive they will have a label similar to: JTXXXX XXXX= primer number XX pM/l : = number 3. Label tubes 4. Add 10 times the number () of milli-Q H2O to the tube to obtain a ~100 pmol/l concentration. These are the stock tubes. Store at -20C . We routinely use 5pmol/l of primer/25l rxn