OHS017
OHS Risk Assessment and
Control Form
Risk assessment completed by:Dr S. Palmer
Staff/student number: 3283213
School/Unit: School of Medical Science, Neuromuscular and Regenerative Medicine
Research Unit
Faculty/Division: Medicine
Document number
SOMS.CGM.RA046
Initial Issue date
30/06/09
Current version
1.0
Current Version
Issue date 30/06/09
Next review date
30/06/12
For additional information refer to the OHS Risk Assessment and Control Procedure, the OHS Risk Rating Procedure and the Hierarchy of Risk Controls.
Risk Assessment title:: Quantitative PCR using the Stratagene Mx3000P instrument
Step 1: Identify the activity
Describe the activity:
Prepare the reaction mix using the pre-made Brilliant II SYBR Green QPCR master mix
Nuclease-free PCR-grade water to allow the final volume to reach 25 μl (including experimental DNA) – Do not add template at this stage
12.5 μl of 2× master mix
x μl of upstream primer (50–150 nM final concentration is recommended)
x μl of downstream primer (50–150 nM final concentration is recommended)
Template DNA
Pipette into 96well reaction plate
Using the Stratagene Mx3000P instrument
Open the door located on the front of the instrument by sliding it all the way to the top. To expose the
thermal block, pull forward on the hot-top handle and lift the hot-top up and away from the thermal block.
Place your plate in the plate holder with the last row (row H) facing front. Well A1 should be positioned at
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the top-left corner of the thermal block. Make sure the plate is properly aligned in the holder. Close the
hot-top assembly by pressing down the hot-top and pushing the handle back into its original place. Slide
down the door to close.
Describe the location:
Laboratory Rm 501, Fume hood Rm 525, Yeast shaking incubator Rm 525
Step 2: Identify who may be at risk by the activity
A number of people may be at risk from any activity. This may affect the risk controls needed. These people may include fellow workers, visitors, contractors and the public. The location of the activity
may affect the number of people at risk.
Operator
Steps 3 to 7: Identify the hazards, risks, and rate the risks
1. An activity may be divided into tasks. For each task identify the hazards and associated risks.
2. List existing risk controls and determine a risk rating using the UNSW Risk Rating Procedure.
3. Additional risk controls may be required to achieve an acceptable level of risk. Re-rate the risk if additional risk controls used.
Tasks
Hazards
Associated risks
(Step 3)
(Step 4)
Risk rating with
existing controls *
Additional risk controls
required
Risk Rating with
additional controls *
(Step 5)
(Step 6)
(Step 7)
Existing risk controls
C
Make master mix
and dispense into
96well plate with
template
Load and run
machine
Physical Hazard
Microcentrifuge
Physical Hazard
QPCR machine
Microcentrifuge
electrocution
Rotor spin injury
Standard PPE lab coat,
goggles and gloves.
L
R
2
E
L
2
E
L
(Apply the hierarchy of
risk controls)
C
L
R
Electrical equipment testing
electrocution
Burn from PCR block and
Hot-lid
Training
Standard PPE lab coat,
goggles and gloves.
Electrical equipment testing
Training
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* C = consequence
L = likelihood
R = risk rating
from the UNSW Risk Rating Procedure
Step 8 Documentation and supervisor approval
Completed by: (name)
S.Palmer
(signature)
Authorised by: Edna Hardeman
(signature)
Date:Jun09
Step 9: Implement the additional risk controls
identified
Indicate briefly what additional risk controls from Step 6 above were implemented, when and by whom.
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Step 10: Monitor and review the risk controls
It is important to monitor risk controls and review risk assessments regularly. Review is required when there is a change in the process, relevant legal changes, and where a cause for concern has
arisen. Reviews could be scheduled on an annual basis. If the risk assessment has substantially changed a new risk assessment is warranted.
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Documentation
It is a requirement that legal and advisory documentation that supports this risk assessment be listed. Such documentation includes Acts, Regulations, Australian Standards and Codes of Practice,
where applicable.
NSW OHS Act 2000
NSW OHS Regulation 2001
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
AS/NZS 2243.3-2002. Safety in laboratories. Part 3: Microbiological aspects and containment facilities.
AS/NZS 2243.4: 1998 Safety in Laboratories Part 4; Ionising Radiations
AS/NZS 2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
AS/NZS 2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
AS/NZS 1336:1997 Recommended Practices for Occupational Eye Protection
UNSW Hazardous Waste Disposal Procedure
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UNSW Concise OHS Risk Rating Table
OHS697
What you need to do
1. Consider what can go wrong that can hurt someone
2. Determine what the most likely outcome would be - Consequences
3. Determine how likely those consequences are - Likelihood
4. Calculate the risk rating
5. Required action
How severely could someone be hurt
death or permanent disability to one or more persons
hospital admission required
medical treatment required
first aid required
injuries not requiring first aid
CONSEQUENCES:
Severe
Major
Moderate
Minor
Insignificant
How likely are those consequences?
expected to occur in most circumstances
will probably occur in most circumstances
could occur at some time
is not likely to occur in normal circumstances
may occur only in exceptional circumstances
LIKELIHOOD:
Almost certain
Likely
Possible
Unlikely
Rare
CONSEQUENCES
Insignificant
1
Minor
2
Moderate
3
Major
4
Severe
5
M
H
H
VH
VH
M
M
H
H
VH
Possible
C
L
M
H
H
VH
Unlikely
D
L
L
M
M
H
Rare
E
L
L
M
M
M
LIKELIHOOD
Almost
certain
A
Likely
B
Risk level
Very high
High
Medium
Low
Required action
Act immediately:
The proposed task or process activity must not proceed. Steps must be taken to lower the risk level to as
low as reasonably practicable using the hierarchy of risk controls.
Act today:
The proposed activity can only proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk controls must include those identified in legislation, Australian Standards, Codes of
Practice etc.
(iii) the risk assessment has been reviewed and approved by the Supervisor and
(iv) a Safe Working Procedure or Safe Work Method has been prepared.
(v) The supervisor must review and document the effectiveness of the implemented risk
controls.
Act this week:
The proposed task or process can proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk assessment has been reviewed and approved by the Supervisor and
(iii) a Safe Working Procedure or Safe Work Method has been prepared.
Act this month:
Managed by local documented routine procedures which must include application of the hierarchy of
controls.
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UNSW Concise OHS Risk Rating Table
Effective date: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6,16/07/2008