OHS017
OHS Risk Assessment and
Control Form
Risk assessment completed by: Teresa Bonello
Staff/student number: 3286547
Faculty/Division: Medicine
Document number
CGM RA001
School/Unit: School of Medical Sciences
Initial Issue date
26 June 2009
Current version
V 1.0
Current Version
Issue date
26-June-2009
Next review date
26-June-2012
For additional information refer to the OHS Risk Assessment and Control Procedure, the OHS Risk Rating Procedure and the Hierarchy of Risk Controls.
Risk Assessment title:: AlamarBlue cell viability assay
Step 1: Identify the activity
1.
Plate mammalian cells at the required density in a 96 well plate, and allow cells to adhere for 24hrs at 37°C and 5% CO2 before proceeding with the assay.
1a. Optional*: Treat cells with cytotoxic compound of interest, diluted serially over an 8 log range of concentrations.
2.
Add 1/10th volume of alamarBlue® reagent directly to the cells in culture medium (alamarBlue is a 10x solution)
3.
Incubate at 37°C in a cell culture incubator, taking fluorescent readings at 3-4hrs (baseline) and 24, 48 and 72hrs.
4.
Measure fluorescence excitation wavelength at 540-570nm (peak excitation 570nm). Read fluorescence emission at 590-610nm (peak emission is 585nm).
5.
Subtract the average fluorescence values of the control group (media only) from the fluorescence of the experimental groups (5a. vehicle control group from the drugtreated groups).
6.
Determine cell viability by plotting fluorescence emission intensity versus log compound concentration. Larger fluorescence emission intensity values correlate to an
increase in total metabolic activity from cells.
* See: SWP titled “handling cytotoxic compounds”
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Page 1 of 4
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
Describe the location: Wallace Wurth Building, Level 5, Rm 502 laboratory [tissue culture room]/ Rm 511 laboratory [plate reader measurements]
Step 2: Identify who may be at risk by the activity
A number of people may be at risk from any activity. This may affect the risk controls needed. These people may include fellow workers, visitors, contractors and the public. The location of the activity
may affect the number of people at risk.
Experimental operator: Staff member, student
Other laboratory staff/students
Visitor/contractor
Steps 3 to 7: Identify the hazards, risks, and rate the risks
1. An activity may be divided into tasks. For each task identify the hazards and associated risks.
2. List existing risk controls and determine a risk rating using the UNSW Risk Rating Procedure.
3. Additional risk controls may be required to achieve an acceptable level of risk. Re-rate the risk if additional risk controls used.
Tasks
Hazards
(Step 3)
Plate mammalian cells at the
required density in a 96 well
plate
Mammalian cells
Treat cells with cytotoxic
compound of interest
REFER TO RISK
ASSESSMENT ON
HANDLING CYTOTOXIC
COMPOUNDS
Add 1/10th volume of
alamarBlue® reagent directly
to the cells in culture medium
AlamarBlue
Associated
risks
Additional risk controls
required
Risk Rating with
additional controls *
(Step 5)
(Step 6)
(Step 7)
Existing risk controls
(Step 4)
Infection
Ingestion, skin
or eye contact
* C = consequence
Risk rating with existing
controls *
C
Personal protective
clothing used
Personal protective
clothing used
L = likelihood
R = risk rating
L
R
1
E
L
-
-
-
1
D
L
(Apply the hierarchy of
risk controls)
C
L
R
Not required
Not required
from the UNSW Risk Rating Procedure
Step 8 Documentation and supervisor approval
Completed by: Teresa Bonello
(signature)
Authorised by: Peter Gunning
(signature)
Date: 26/6/09
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Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
Step 9: Implement the additional risk controls
identified
Indicate briefly what additional risk controls from Step 6 above were implemented, when and by whom.
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Step 10: Monitor and review the risk controls
It is important to monitor risk controls and review risk assessments regularly. Review is required when there is a change in the process, relevant legal changes, and where a cause for concern has
arisen. Reviews could be scheduled on an annual basis. If the risk assessment has substantially changed a new risk assessment is warranted.
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Documentation
It is a requirement that legal and advisory documentation that supports this risk assessment be listed. Such documentation includes Acts, Regulations, Australian Standards and Codes of Practice,
where applicable.
NSW OHS Act 2000
NSW OHS Regulation 2001
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
AS/NZS 2243.3: 2006 Safety in laboratories Part 3: Microbiological aspects and containment facilities
AS/NZS 2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
AS/NZS 2243.3:2006 Safety in Laboratories Part 7 Electrical aspects
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
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Page 3 of 4
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
UNSW Concise OHS Risk Rating Table
OHS697
What you need to do
1. Consider what can go wrong that can hurt someone
2. Determine what the most likely outcome would be - Consequences
3. Determine how likely those consequences are - Likelihood
4. Calculate the risk rating
5. Required action
How severely could someone be hurt
death or permanent disability to one or more persons
hospital admission required
medical treatment required
first aid required
injuries not requiring first aid
CONSEQUENCES:
Severe
Major
Moderate
Minor
Insignificant
How likely are those consequences?
expected to occur in most circumstances
will probably occur in most circumstances
could occur at some time
is not likely to occur in normal circumstances
may occur only in exceptional circumstances
LIKELIHOOD:
Almost certain
Likely
Possible
Unlikely
Rare
CONSEQUENCES
Insignificant
1
Minor
2
Moderate
3
Major
4
Severe
5
M
H
H
VH
VH
M
M
H
H
VH
Possible
C
L
M
H
H
VH
Unlikely
D
L
L
M
M
H
Rare
E
L
L
M
M
M
LIKELIHOOD
Almost
certain
A
Likely
B
Risk level
Very high
High
Medium
Low
Required action
Act immediately:
The proposed task or process activity must not proceed. Steps must be taken to lower the risk level to as
low as reasonably practicable using the hierarchy of risk controls.
Act today:
The proposed activity can only proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk controls must include those identified in legislation, Australian Standards, Codes of
Practice etc.
(iii) the risk assessment has been reviewed and approved by the Supervisor and
(iv) a Safe Working Procedure or Safe Work Method has been prepared.
(v) The supervisor must review and document the effectiveness of the implemented risk
controls.
Act this week:
The proposed task or process can proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk assessment has been reviewed and approved by the Supervisor and
(iii) a Safe Working Procedure or Safe Work Method has been prepared.
Act this month:
Managed by local documented routine procedures which must include application of the hierarchy of
controls.
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Page 4 of 4
UNSW Concise OHS Risk Rating Table
Effective date: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6,16/07/2008