CURRICULUM VITAE Primal de Lanerolle THESES: 1. de Lanerolle, P. Ms. Eve: An Analysis of Milton's Heroine. Master's Thesis in English Literature, California State University, San Francisco, 1975. 2. de Lanerolle, P. Myosin Phosphorylation and the Contractile State of Tracheal Smooth Muscle. Doctoral Thesis in Physiology and Pharmacology, University of California, San Diego, 1979. PUBLICATIONS: 1. Osborn, J.J., Russell, J.A.G., Beaumont, J.O., de Lanerolle, P., McChesney, B. and Garfield, F. The measurement of relative stroke volume from aortic pulse contour or pulse pressure. Vascular Disease 5: 165-177, 1968. 2. Hill, J.D., Aguilar, M.J., Baranco, A., de Lanerolle, P. and Gerbode, F. Neuropathological manifestations of cardiac surgery. Ann. Thor. Surg. 7: 409-419, 1969. 3. Hill, J.D., Osborn, J.J., Swank, R.L., Aguilar, M.J., de Lanerolle, P. and Gerbode, F. Experience using a new Dacron wool filter during extracorporeal circulation. Arch. Surg. 101: 649-652, 1970. 4. Angell, W.W., de Lanerolle, P. and Shumway, N.E. Valve replacement: Present status of homograft valves. Prog. Cardiov. Dis. 15: 589-622, 1973. 5. Hill, D.G., de Lanerolle, P., Heiden, D., Aguilar, M.J. and Gerbode, F. Protection from lung damage by blood filtration during deep hypothermia in puppies. J. Thorac. Cardiov. Surg. 70: 133-138, 1975. 6. Hill, D.G., de Lanerolle, P., Kosek, J.C., Aguilar, M.J. and Hill, J.D. The pulmonary pathophysiology of membrane and bubble oxygenators. Trans. Am. Soc. Art. Int. Organs 21: 165-169, 1975. 7. Stull, J.T., Blumenthal, D.K., de Lanerolle, P., High, C.W. and Manning, D.R. Phosphorylation and regulation of contractile proteins. Adv. Pharmac. Therapeutics 3: 171-182, 1978. 8. Stull, J.T., Manning, D.R., High, C.W., de Lanerolle, P. and Blumenthal, D.K. Phosphorylation of contractile proteins by cyclic nucleotide dependent and independent protein kinases, in Catecholamines: Basic and Clinical Frontiers. ed. by E. Usdin, (Pergamon Press, New York), pp. 1161-1163, 1979. 9. de Lanerolle, P. and Stull, J.T. Myosin phosphorylation during contraction and relaxation of tracheal smooth muscle. J. Biol. Chem. 255: 9993-10000, 1980. 10. de Lanerolle, P., Adelstein, R.S., Feramisco, J.R. and Burridge, K. Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in non-muscle cells. Proc. Nat. Acad. Sci. USA 78: 4738-4742, 1981. 11. Feramisco, J.R., Burridge, K., de Lanerolle, P. and Adelstein, R.S. Localization of myosin light chain kinase in non-muscle cells, in Cold Spring Harbor Conference on Cell Proliferation, Vol. 8, ed. by O. Rosen, and E.G. Krebs, (Cold Spring Harbor, NY), pp. 855-868, 1981. 12. de Lanerolle, P., Condit, J.R., Tanenbaum, M. and Adelstein, R.S. Myosin phosphorylation, agonist concentration and contraction of tracheal smooth muscle. Nature 298: 871-872, 1982. 13. Adelstein, R.S., Pato, M.D., Sellers, J.R., de Lanerolle, P. and Conti, M.A. Regulation of the actin-myosin interaction by reversible phosphorylation of myosin kinase, in Cold Spring Harbor Symposium on Quantitative Biology, Vol. XLVI, (Cold Spring Harbor, NY), pp. 921-928, 1982. 14. Adelstein, R.S., de Lanerolle, P., Sellers, J.R., Pato, M.D. and Conti, M.A. Regulation of contractile proteins in smooth muscle and platelets by calmodulin and cyclic AMP, in Recent Advances in Ca2+ and Cell Function, Calmodulin and Intracellular Ca 2+Receptor, (Kyoto, Japan), pp. 313-331, 1982. 15. Adelstein, R.S., Sellers, J.R., Conti, M.A., Pato, M.D. and de Lanerolle, P. Regulation of smooth muscle contractile proteins by calmodulin and cyclic AMP. Fed. Proc. 41: 2873-2878, 1982. 16. Adelstein, R.S., Pato, M.D., Sellers, J.R., de Lanerolle, P. and Conti, M.A. Regulation of contractile proteins by reversible phosphorylation of myosin and myosin kinase, in Basic Biology of Muscle: A Comparative Approach, ed. By B.M. Twarog, R.J.C. Levine and M.M. Dewey, (Raven Press, NY), pp. 273-281, 1982. 17. Donner, L., de Lanerolle, P. and Costa, J. Immunoreactivity of paraffinembedded normal tissues and mesenchymal tumors for smooth muscle myosin. Am. J. Clin. Path. 80: 677-681, 1983. 18. de Lanerolle, P., Nishikawa, M., Yost, D.A. and Adelstein, R.S. Increased phosphorylation of myosin light chain kinase after an increase in cyclic AMP in intact smooth muscle. Science 223: 1415-1417, 1984. 19. Nishikawa, M., de Lanerolle, P., Lincoln, T.M. and Adelstein, R.S. Phosphorylation of mammalian myosin light chain kinases by the catalytic subunit of cyclic AMPdependent protein kinase and by cyclic GMP dependent protein kinase. J. Biol. Chem. 259: 8429-8436, 1984. 20. Barany, K., Csabina, S., de Lanerolle, P. and Barany, M. Evidence for isoforms of the phosphorylatable myosin light chain in rat uterus. Biochim. Biophys. Acta. 911: 369371, 1987. 21. TomMoy, M., Madison, J.M., Jones, C.A., de Lanerolle, P. and Brown, J.K. Morphological characterization of smooth muscle cells isolated from the tracheas of adult dogs. Anatomical Record 218: 313-328, 1987. 22. de Lanerolle, P., Nishikawa, M., Felsen, R. and Adelstein, R.S. Immunological properties of myosin light chain kinase. Biochim. Biophys. Acta., 914: 74-82, 1987. 23. de Lanerolle, P. Myosin dephosphorylation and isometric relaxation of tracheal smooth muscle. J. Appl. Physiol., 64: 705-709, 1988. 24. de Lanerolle, P. and Nishikawa, M. Regulation of fetal smooth muscle myosin by protein kinase C. J. Biol. Chem., 263: 9071-9074, 1988. 25. Bissonnette, M.P., Kuhn, D. and de Lanerolle, P. Purification and characterization of myosin light chain kinase from rat pancreas. Biochem. J., 258: 739-747, 1989. 26. Obara, K. and de Lanerolle, P. Effect of isoproterenol on myosin phosphorylation and the contractile properties of tracheal muscle. J. Appl. Physiol., 66: 2017-2022, 1989. 27. Obara, K., Takai, A., Ruegg, J.C. and de Lanerolle, P. Okadaic acid, a phosphatase inhibitor, produces a Ca2+ and calmodulin-independent contraction of lamb tracheal smooth muscle. Pfluger's Archiv., 414: 134-138, 1989. 28. de Lanerolle, P. Regulation of airway smooth muscle responses, in Lung Biology in Health and Disease, Vol. 41, (ex editor C. Lenfant), ed. by D. Massaro (Marcel Dekker, Inc., N.Y.), pp. 153-189, 1989. 29. de Lanerolle, P. Cellular control mechanisms in airway smooth muscle, in Airway Smooth Muscle in Health and Disease, ed. by R.F. Coburn (Plenum Press, N.Y.), pp. 99-126, 1989. 30. de Lanerolle, P. Regulation of embryonic smooth muscle myosin by myosin light chain kinase and protein kinase C, in Calcium Protein Signaling, ed. by H. Hidaka (Plenum Press, NY), pp. 305-314, 1989. 31. de Lanerolle, P. and Obara, K. Regulation of smooth muscle by myosin phosphorylation, in Muscle Energetics, ed. by R.J. Paul, G. Elzinga and K.Yamada (A.R. Liss, Inc., NY), pp. 293-304, 1989. 32. Wilson, A.K., Takai, A., Ruegg, J.C. and de Lanerolle, P. Okadaic acid, a phosphatase inhibitor, decreases macrophage motility. Am. J. Physiol., 260: L105-L112, 1991. 33. de Lanerolle, P., Strauss, J.D., Felsen, R. Doerman, G.E. and Paul, R.J. The effects of antibodies to myosin light chain kinase on contractility and myosin phosphorylation in chemically permeabilized smooth muscle. Circulation Res., 68: 457-465, 1991. 34. Wilson, A.K., Horowitz, J. and de Lanerolle, P. Evaluation of the electroinjection method for introducing proteins into living cells. Am. J. Physiol., 260: C355-C363, 1991. 35. Wilson, A.K., Gorgas, G., Claypool, W.D. and de Lanerolle, P. An increase or a decrease in myosin II phosphorylation inhibits macrophage motility. J. Cell Biology 114: 277-283, 1991. 36. de Lanerolle, P. and Paul, R.J. Myosin phosphorylation/dephosphorylation and the regulation of airway muscle contractility. Am. J. Physiol., 258: L1-L14, 1991. 37. Paul, R.J., Strauss, J.D. and de Lanerolle, P. Peptides as probes of the mechanisms regulating smooth muscles: Studies on skinned fibers, in Peptides as Probes in Muscle Research, ed. by J.C. Ruegg (Springer-Verlag, Heidelberg), pp. 111-117, 1991. 38. de Lanerolle, P., Strauss, J.D. and Paul, R.J. Antibodies as probes of the mechanisms regulating smooth muscle contractility: Studies on skinned fibers, in Peptides as Probes in Muscle Research, ed. by J.C. Ruegg (Springer-Verlag, Heidelberg), pp. 119-129, 1991. 39. Strauss, J.D., de Lanerolle, P. and Paul, R.J. The effects of myosin kinase inhibiting peptide on contractility and myosin phosphorylation in chemically skinned smooth muscle. Am. J. Physiol., 262: C1437-1445, 1992. 40. Wilson, A.K., Pollenz, R.S., Chisholm, R.L. and de Lanerolle, P. The role of myosin I and II in cell motility. Cancer and Metastasis Reviews 11: 79-91, 1992. 41. de Lanerolle, P. Airway Smooth Muscle and asthma. Am. J. Respir. Cell Mol. Biol. 7: 565-566, 1992. 42. de Lanerolle, P., Gorgas, G., Li, X. and Schluns, K. Myosin light chain phosphorylation does not increase during yeast phagocytosis by macrophages. J. Biol. Chem., 268: 16,883-16,886, 1993. 43. de Lanerolle, P., Gorgas, G., Dezso, B., Schluns, K. and Wilson, A.K. The role of myosin phosphorylation in smooth muscle and macrophage signalling, in Lung Biology in Health and Diseases, (ex. ed. C. Lenfant) ed. by J. Brody, D. Center and V. Tkachuck, (Marcel Dekker, Inc., NY.), pp. 351-368, 1993. 44. Cai, S., Nowak, G. and de Lanerolle, P. Myosin dephosphorylation as a mechanism of relaxation in Airways Smooth Muscle: A Reference Source ed. By M.A. Giembycz and D. Raeburn (Birkhauser, Basel), pp. 233-251, 1994. 45. Obara, K., Nikcevic, G., Pestic., L., Nowak, G., Guerriero, V., Paul, R.J., Elson, E.L. and de Lanerolle, P. Fibroblast contractility without an increase in basal myosin light chain phosphorylation in wild type cells and cells expressing the catalytic domain of myosin light chain kinase. J. Biol. Chem. 270: 18,734-18,737, 1995. 46. Christensen, S., Verhage, H.G., Nowak, G., de Lanerolle, P., Fleming, S., Bell, S.C. Smooth muscle myosin II and alpha smooth muscle actin expression in the baboon (papio anubis) uterus is associated with glandular secretory activity and stromal cell transformation. Biology of Reproduction 53: 596-606, 1995. 47. Lorimer, D.D. and de Lanerolle, P. Myosin in Principles of Medical Biology ed. by E.E. Bitar and N. Bitar, (JAI Press, Greenwich, CT), pp. 59-66, 1996. 48. Paul, R.J. and de Lanerolle, P. Regulation of smooth muscle contractility, in Genetics of Asthma, ed. by S. Liggett and D. Meyers (Marcel Dekker, Inc., NY), pp. 91-117, 1996. 49. Hecht, G.A., Pestic, L., Nikcevic, G., Koutsouris, A., Tripuraneni, J., Lorimer, D.D., Nowak, G., Guerriero, V., Elson, E. and de Lanerolle, P. Expression of the catalytic domain of myosin light chain kinase increases paracellular permeability. Am. J. Physiol., 271: C1675-C1684, 1996. 50. Borhani, M., Nikcevic, G., Eton, D., Cava, R.A., Landau, D.S., Pestic, L., Baraniewski, H., Schuler, J.J. and de Lanerolle, P. Altering the cytoskeleton through gene therapy inhibits intimal hyperplasia in a porcine model. Surgical Forum XLVII: 339-341, 1996. 51. Gandhi, S., Lorimer, D.D. and de Lanerolle, P. Expression of a mutant myosin light chain that cannot be phosphorylated increases paracellular permeability. Am. J. Physiol., 272: F214-F221, 1997. 52. Tripuraneni, J., Koutsouris, A., Pestic, L., de Lanerolle, P. and Hecht, G. Okadaic acid, the toxin responsible for diarrheic shellfish poisoning, increases intestinal epithelial paracellular permeability. Gastroenterology 112: 100-108, 1997. 53. Klemke, R.L., Cai, S., Giannini, A.L., Gallagher, P., de Lanerolle, P. And Cheresh, D.A. Regulation of cell motility by mitogen activated protein kinase. J. Cell Biol., 137: 481-492, 1997. 54. de Lanerolle, P. Ibuprofen inhibits coronary collateral development and self induced endothelial cell growth. Cardiovascular Pathobiol., 1: 79-80, 1997. 55. Nowak, G., Pestic-Dragovich, L., Hozak, P., Philimonenko, A., Simerly, C., Schatten, G. and de Lanerolle, P. Evidence for the presence of myosin I in the nucleus. J. Biol. Chem., 272: 17,176-17,181, 1997. 56. Majumdar, M., Seaholtz, T.M., Goldstein, D., de Lanerolle, P. and Brown, J.H. Thrombin-induced cell rounding is mediated through Rho and requires myosin light chain phosphory-lation. J. Biol. Chem., 273: 10,099-10,106, 1998. 57. Simerly, C., Nowak, G., de Lanerolle, P. and Schatten, G. Differential expression and functions of cortical myosin IIA and IIB isotypes during meiotic maturation, fertilization and mitosis in mouse oocytes and embryos. Mol. Biol. Cell 9: 2509-2525, 1998. 58. Cai, S., Pestic-Dragovich, L., O'Donnell, M.E., Wang, N., Ingber, D., Elson, E. and de Lanerolle, P. Regulation of cytoskeletal dynamics and cell growth by myosin light chain phosphorylation. Am. J. Physiol., 275: C1349-C1356, 1998. 59. Sanders, L., Matsumura, F., Bokoch, G.M. and de Lanerolle, P. Inhibition of myosin light chain kinase by p21-activated kinase (PAK) during cell spreading. Science 283: 2083-2085, 1999. 60. Pestic-Dragovich, L., Stojiljkovic, L., Philimonenko, A., Nowak, G., Ke, Y., Settlage, R.E., Shabanowitz, J., Hunt, D.F., Hozak, P. and de Lanerolle, P. A myosin I isoform in the nucleus. Science 290: 337-341, 2000 61. Stojiljkovic, L., Pestic-Dragovich, L. and de Lanerolle, P. Nuclear myosin I ß. GenBank Accession Number AY 007255, 2000. 62. Obara, K., Nobe, K., Nobe, H., Kolodney, M.S., de Lanerolle, P. and Paul, R.J. Nonmuscle motility: Differential effects of microfilament disruption on force, velocity, stiffness and [Ca2+]I in a reconstituted fibroblast fiber. Am. J. Physiol., 279: C785C790, 2000 63. Tinsley, J.H., de Lanerolle, P., Wilson, E. and Yuan, S.Y. Myosin light chain kinase redistribution and activation of myosin light chain regulate endothelial barrier function. Am. J. Physiol., 279: C1285-C1289, 2000. 64. Rathman, M., de Lanerolle, P., Ohayon, H., Gounon, P. and Sansonetti, P. Myosin light chain kinase plays an essential role in S. flexneri dissemination. J. Cell Science, 113: 3375-3386, 2000. 65. Cornwell, T.L., Li, J., Sellak, H., de Lanerolle, P., Rodgers, W.H., Miller, R.T. and Word, R.A. Regulation of cyclic guanosine monophosphate-dependent protein kinase in human uterine tissues during the menstrual cycle. Biol. of Reproduction 64: 857864, 2001. 66. Carr, A.N., Sutliff, R.L., Weber, C.S., Allen, P.B., Greengard, P., de Lanerolle, P., Kranias, E.G. and Paul, R.J. Is myosin phosphatase regulated in vivo by inhibitor-1? Evidence from inhibitor-1 knockout mice. J. Physiology, in press. 67. Qu., J., Cammarano, M.S., Shi, Q, Ha, K.C., de Lanerolle, P. and Minden, A. Activated PAK 4 regulates cell adhesion and anchorage independent growth. Mol. Cell. Biol., 21: 3523-33, 2001. 68. Nobe, H., Nobe, K., Fazal, F., de Lanerolle, P. and Paul, R.J. Rho-associated kinase mediates calf serum induced contraction of NIH 3T3 fibroblast fibers without increasing myosin light chain phosphorylation. Submitted for publication. 69. de Lanerolle, P., Fazal, F., Lee, B-S.B., Bokoch, G.M., Ficarro, S.B., Shabanowitz, J. and Hunt, D.F. Phosphorylation and regulation of myosin light chain kinase by PAK and ERK. In preparation. 70. Stojiljkovic, L., Fan, J-L., Philimonenko, V.V., Kysela, K.,Goodrich, J.A., Lessard, J.L., Hope, T.J., Hozak, P. and de Lanerolle, P. A role for nuclear myosin I and ß-actin in transcription by RNA polymerase II. In preparation. 71. Nobe, H., Nobe, K., Fazal, F., de Lanerolle, P. and Paul, R.J. Rho-associated kinase mediates calf serum induced contraction of NIH 3T3 fibroblast fibers without increasing myosin light chain phosphorylation. Am J Physiol. 284: C599-606, 2003. 72. Wirth, A., Schroeter, M., Manser, E., de Lanerolle, P. and Pfitzer, G. Inhibition of smooth muscle contraction and myosin light chain phosphorylation by p21-activated kinase PAK1. J. Physiol., 549: 489-500, 2003. 73. Pfitzer, G., Wirth, A., Lucius, C., Brkic-Koric, D., Manser, E., de Lanerolle, P. and Arner, A. Regulation of smooth muscle contraction by calcium, monomeric GTPases of the Rho subfamily and their effector kinases. Adv Exp Med Biol. 538: 89-99, 2003. 74. Chou, C-L., Christensen, B.M., Frische, S., Vorum, H., Desai, R.A., Hoffert, J.D., de Lanerolle, P., Nielsen, S. and Knepper, M.A. Nonmuscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting duct. J. Biol. Chem., 279: 49,026-49.035, 2004. 75. Hofmann, W.A., Ljuba Stojiljkovic, L., Fuchsova, B., Vargas, G.M., Mavrommatis, E., Philimonenko, V., Kysela, K., Goodrich, J.A., Lessard, J.L., Hope, T.J., Hozak, P. and de Lanerolle, P. Actin is part of pre-initiation complexes and necessary for transcription by RNA polymerase II. Nature Cell Biology 6: 1094-1101, 2004. 76. Philimonenko, V.V., Zhao, J., Iben, S., Dingova, H., Kysela, K., Kahle, M., Zentgraf, H., Hofmann, W.A., de Lanerolle, P., Hozak, P. and Grummt, I. Nuclear actin and myosin I are required for RNA polymerase I transcription. Nature Cell Biology 6: 1165-1172, 2004. 77. de Lanerolle, P. Top ten tips for success in graduate school. Nature 433: 442, 2005 78. Fazal, F., Gu, L-Z., Ihnatovych, I., Han, YJ., Antic, N., Hu, W-Y., Carriera, F., Blomquist, J.F., Hope, T.J., Ucker, D. and de Lanerolle, P. Inhibiting myosin light chain kinase induces apoptosis in vitro and in vivo. Mol. Cell. Biology 25: 6259-6266, 2005. 79. de Lanerolle, P., Johnson, T. and Hofmann, W.A. Actin and myosin I in the nucleus: What next? Nature Struct. and Molec. Biol., 12: 292-296, 2005. 80. Hofmann, W. and de Lanerolle, P. Nuclear actin: To polymerize or not to polymerize. J. Cell Biology, 172: 495-496, 2006. 81. Chuang, C-H., Carpenter, A.E., Fuchsova, B., Johnson, T., de Lanerolle, P. and Belmont, A.S. Long-range directional movement of an interphase chromosome site. Current Biology 16: 825-831, 2006. 82. Hofmann, W.A. and de Lanerolle, P. "Actin, Actin-Related Proteins and Actin-Binding Proteins in Transcriptional Control" in Gene Expression and Regulation (ed. J. Ma), a Current Scientific Frontiers Book, Higher Education Press (Beijing), 2006. 83. Gu, L., Hu, W-Y., Antic, N., Mehta, R., Turner. J.R. and de Lanerolle, P. Inhibiting Myosin Light Chain Kinase Retards the Growth of Mammary and Prostate Cancer Cells, In Vitro and In Vivo. European Journal of Cancer 42: 948-957, 2006. Epub Mar 30, 2006. 84. Hofmann, W.A., Vargas, G.M., Ramchandran, R., Goodrich, J.A. and de Lanerolle, P. Nuclear myosin I is necessary for the formation of the first phosphodiester bond during transcription initiation by RNA polymerase II. J. Cellular Biochemistry, in press. 85. Hofmann, W.A., Johnson, T., Fan, J-L, Klapczynski, M. and de Lanerolle, P. From transcription to transport: Emerging roles for nuclear myosin I. Biochemistry and Cell Biology, in press. 86. Han, Y-J., Hu, W-Y., Antic, N., Gu, L., Gupta, M., Piano, M. and de Lanerolle, P. Increased myosin light chain kinase expression in hypertension: Regulation by SRF via an insertion mutation in the promoter. Submitted. 87. Hu, W-Y., Han, Y-J., Gu, L-Z., Piano, M. and de Lanerolle, P. Involvement of myosin light chain phosphorylation in the captopril effects in spontaneously hypertensive rats. Submitted.