® HLA-B*82 101.552-06/06u 71Y August 2015 Rev. No: 00 Visit www.olerup-ssp.com for “Instructions for Use” (IFU) and detailed Product Inserts Gel Documentation Form and Worksheet HLA-B*82 (101.552-06/06u) Lot: 71Y Expiry Date: 2018-February-01 Name:____________________ Test Date: _______________ Review Date:_______________ Sample ID:________________ Tested By: _______________ Reviewed By:_______________ DNA Conc.(ng/ul):___________ Interpretation:___________ Failed lanes: ___________ Comments:_________________ Gel Picture PHOTO DOCUMENT ICB Am pS Well No. Specific Reaction 1 2 3 4 5 6 800 800 1070 1070 1070 195 140 230 195 155 NC Well No. 1 2 3 4 5 6 ‘ICB’ Internal Control Band, ‘AmpS’ Amplicon size Notes: Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table. This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table. Primer mixes 2 and 3 may have tendencies of unspecific amplifications. Primer mix 2 has a tendency to giving rise to primer oligomer formation. Primer mix 6 contains a negative control, which will amplify more than 95% of HLA amplicons as well as the amplicons generated by the control primer pairs matching the human growth hormone gene. HLA-specific PCR product sizes range from 75 to 200 base pairs and the PCR product generated by the HGH positive control primer pair is 430 base pairs. Olerup SSP AB Franzengatan 5 SE-112 51 Stockholm Sweden For In Vitro Diagnostic Use Page 1 of 2 ® HLA-B*82 101.552-06/06u 71Y Worksheet Well No. HLA-B allele1,2 *82:01 *82:02:01-82:02:02 *82:03 *15:06, 15:27:01-15:27:03, 15:84, 15:109, 15:195, 15:327, 15:344, C*03:89, C*03:271, C*04:08, C*04:34, C*04:147, C*18:08 *44:10, 44:15, 44:18, 44:140, 45:01:0145:01:02, 45:05-45:07, 45:11-45:15, 49:20, 50:02 *51:01:28 Well No. 1 2 1 1 1 2 3 4 5 6 3 4 4 4 5 Negative Control August 2015 Rev. No: 00 4 4 5 6 2 3 1 1 2 3 Visit www.olerup-ssp.com for “Instructions for Use” (IFU) and detailed Product Inserts 1HLA-B*82 alleles in bold lettering are listed as confirmed alleles on the IMGT/HLA web page www.ebi.ac.uk/imgt/hla, release 3.20.0 April 2015. 2Alleles that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last IMGT/HLA database release can be retrieved from web page http://hla.alleles.org/alleles/deleted.html. The HLA-B*82 subtyping kit cannot distinguish the following silent mutations: the B*82:02:01 and 82:02:02 alleles. Olerup SSP AB Franzengatan 5 SE-112 51 Stockholm Sweden For In Vitro Diagnostic Use Page 2 of 2