HLA-B82 2015 71Y worksheet

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®
HLA-B*82
101.552-06/06u
71Y
August 2015
Rev. No: 00
Visit www.olerup-ssp.com
for “Instructions for Use”
(IFU) and detailed Product
Inserts
Gel Documentation Form and Worksheet
HLA-B*82 (101.552-06/06u)
Lot: 71Y
Expiry Date: 2018-February-01
Name:____________________
Test Date: _______________
Review Date:_______________
Sample ID:________________
Tested By: _______________
Reviewed By:_______________
DNA Conc.(ng/ul):___________
Interpretation:___________
Failed lanes: ___________
Comments:_________________
Gel Picture
PHOTO DOCUMENT
ICB
Am pS
Well No.
Specific
Reaction
1
2
3
4
5
6
800
800 1070 1070 1070
195
140
230
195
155
NC
Well No.
1
2
3
4
5
6
‘ICB’ Internal Control Band,
‘AmpS’ Amplicon size
Notes: Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table.
This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table.
Primer mixes 2 and 3 may have tendencies of unspecific amplifications.
Primer mix 2 has a tendency to giving rise to primer oligomer formation.
Primer mix 6 contains a negative control, which will amplify more than 95% of HLA amplicons as well as the amplicons
generated by the control primer pairs matching the human growth hormone gene. HLA-specific PCR product sizes range
from 75 to 200 base pairs and the PCR product generated by the HGH positive control primer pair is 430 base pairs.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 1 of 2
®
HLA-B*82
101.552-06/06u
71Y
Worksheet
Well No.
HLA-B allele1,2
*82:01
*82:02:01-82:02:02
*82:03
*15:06, 15:27:01-15:27:03, 15:84,
15:109, 15:195, 15:327, 15:344,
C*03:89, C*03:271, C*04:08, C*04:34,
C*04:147, C*18:08
*44:10, 44:15, 44:18, 44:140, 45:01:0145:01:02, 45:05-45:07, 45:11-45:15,
49:20, 50:02
*51:01:28
Well No.
1
2
1
1
1
2
3
4
5
6
3
4
4
4
5
Negative Control
August 2015
Rev. No: 00
4
4
5
6
2
3
1
1
2
3
Visit www.olerup-ssp.com
for “Instructions for Use”
(IFU) and detailed Product
Inserts
1HLA-B*82
alleles in bold lettering are listed as confirmed alleles on the IMGT/HLA web page
www.ebi.ac.uk/imgt/hla, release 3.20.0 April 2015.
2Alleles that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last
IMGT/HLA database release can be retrieved from web page http://hla.alleles.org/alleles/deleted.html.
The HLA-B*82 subtyping kit cannot distinguish the following silent mutations: the B*82:02:01 and 82:02:02 alleles.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 2 of 2
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