®
HLA Wipe Test – Negative Control
102.101-01/01u
36V
February 2014
Rev. No: 00
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
Gel Documentation Form and Worksheet
HLA Wipe Test Negative Control (102.101-01/01u)
Lot No: 36V
Expiry Date: 2016-August-01
Name:___________________
Test Date: ______________
Review Date:________________
Sample ID:_______________
Tested By: ______________
Reviewed By:________________
DNA Conc.(ng/ul):_________
Gel Picture
PHOTO DOCUMENT
Interpretation:_______________
Failed lanes: ___________
Comments___________________
Notes:
For interpretation always use the Product Insert.
In the positive control lane one or several PCR products should be seen. The sizes may range from 75 to 430 bp.
In the negative control lane no PCR product should be seen. Primer oligomer artifacts, approximately 40 to 50 bp in size, may
be seen. This does not represent contamination.
Presence of PCR product(s) 75 to 430 bp in size in the sample lanes without Positive Control DNA indicates contamination.
Absence of PCR product in the sample lanes without Positive Control DNA shows that no detectable contamination is present.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 1 of 2
®
HLA Wipe Test – Negative Control
102.101-01/01u
36V
Worksheet
February 2014
Rev. No: 00
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
HLA-specific PCR product sizes range from 75 to 200 base pairs.
The PCR product generated by the positive control primer pair is 430 base pairs.
Length of PCR
product
105
1
200
164
5'-primer
5'
-CAC3'
105
340
5'
-Agg3'
80
440
5'
-TTA3'
75
45
5'
-Tgg3'
80
45
5'
-Tgg 3'
85
43
5'
-Tgg3'
36
5'
-TAC3'
5'
-TAT3'
36
3'-primer2
231
5'
-TgC
3'
2nd I
5'
-AAA3'
507
5'
-TTg 3'
59
5'
-CTC3'
58
5'
-ggC3'
57
5'
-CTC3'
47
5'
-ACA3'
48
5'
-gCA3'
5'
-gCC3'
48
52
5'
A*
B*
C*
DRB1
DRB3
DRB5
DQB1
DPB1
DQA1
+
+
+
+
+
+
-TgT 3'
+
+
+
+
+
+
+
+
+
+
+
1The
nucleotide position for HLA class I genes and the codon for HLA class II genes, in the 2nd or 3rd exon, matching the specificitydetermining 3'-end of the primer is given. Nucleotide and codonnumbering as on the www.ebi.ac.uk/imgt/hla web site. The sequence
of the 3 terminal nucleotides of the primer is given.
2The nucleotide position for HLA class I genes and the codon for HLA class II genes, in the 2nd or 3rd exon or the 2nd intron,
matching the specificity-determining 3'-end of the primer is given in the anti-sense direction. Nucleotide and codon numbering as
on the www.ebi.ac.uk/imgt/hla web site. The sequence of the 3 terminal nucleotides of the primer is given.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 2 of 2