®
HLA-B*81
101.553-06/06u
99X
April 2015
Rev. No: 00
Visit www.olerup-ssp.com
for “Instructions for Use”
(IFU) and detailed Product
Inserts
Gel Documentation Form and Worksheet
HLA-B*81 (101.553-06/06u)
Lot: 99X
Expiry Date: 2017-October-01
Name:__________________
Test Date: ____________
Review Date:_______________
Sample ID:______________
Tested By: ____________
Reviewed By:_______________
DNA Conc.(ng/ul):________
Interpretation:___________
Failed lanes: ____________
Comments:________________
Gel Picture
PHOTO DOCUMENT
1
2
3
4
175
410
405
285
AmpS
Well No.
Specific
Reaction
5
6
7
8
9
800 1070 1070 1070 1070 1070 1070 1070
1
2
3
4
105
80
165
105
5
6
165
140
NC
Well No.
ICB
7
8
9
‘ICB’ Internal Control Band,
‘AmpS’ Amplicon size
Notes: Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table.
This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table.
HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR products.
Primer mixes 3 and 5 may have tendencies of unspecific amplification.
Primer mix 9 contains a negative control, which will amplify more than 95% of HLA amplicons as well as the amplicons
generated by the control primer pairs matching the human growth hormone gene. HLA-specific PCR product sizes range
from 75 to 200 base pairs and the PCR product generated by the HGH positive control primer pair is 430 base pairs.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 1 of 3
®
HLA-B*81
101.553-06/06u
99X
Worksheet
Well No.
HLA-B allele 1,2,3
*81:01
4
*81:02, 07:174, 07:202, 07:222
*81:03
*81:04N
*81:05
*81:06
*81:07
*07:02:01-07:02:38, 07:02:40-07:02:45, 07:04-07:06, 07:09-07:11, 07:15, 07:17, 07:2007:26, 07:28-07:31, 07:34-07:36, 07:39, 07:41-07:46, 07:48-07:49N, 07:51-07:52, 07:5407:59, 07:61-07:64, 07:66-07:68:03, 07:70-07:76, 07:79-07:82, 07:86-07:99, 07:10107:109, 07:111N-07:121, 07:123-07:136:02, 07:138, 07:140-07:161N, 07:164, 07:16607:173, 07:175-07:179, 07:181N-07:192, 07:194-07:195, 07:199-07:201N, 07:203-07:206,
07:208-07:213, 07:215-07:218, 07:220-07:221, 07:223-07:224, 07:226, 07:229-07:231N,
07:233-07:234, 07:236-07:238, 07:240-07:241, 42:05:01-42:05:02
*07:02:39, 07:07, 07:12, 07:14, 07:18:01-07:19, 07:33:01-07:33:03, 07:40, 07:53, 07:60,
07:77-07:78, 07:84, 07:100, 07:122, 07:137, 07:139, 07:162-07:163, 07:165, 07:193,
07:196-07:198, 07:214, 07:225, 07:227, 07:235, 07:239, 08:123, 08:132, 14:21, 38:26,
42:01:01-42:02, 42:04, 42:06, 42:08-42:10, 42:12-42:20, 45:06, 67:01:01-67:01:03, 67:0367:04, 73:01-73:02
*07:03, 07:08, 07:16, 07:27, 07:32, 07:37-07:38, 07:47, 07:50, 07:69, 07:85, 07:180,
07:207, 07:219, 07:228, 07:232, 08:79, 37:07, 40:15-40:16, 40:32, 40:98, 48:05, 48:08,
48:10, 48:12, 48:14
*07:13, 07:110
*07:65
*07:83
*15:20, 15:228, 15:305, 35:01:01:01-35:01:27, 35:01:29-35:30, 35:32:01-35:34, 35:3635:42:02, 35:44-35:45, 35:47-35:56, 35:58-35:59, 35:61:01-35:65Q, 35:67-35:72, 35:7435:75, 35:77-35:78, 35:80-35:86, 35:88-35:184, 35:187-35:228, 35:230-35:278, 48:02:0148:02:03, 51:01:01:01-51:24:05, 51:26-51:41N, 51:43-51:46, 51:48-51:156, 51:158:0151:163, 51:165-51:185, 52:01:01:01-52:06:02, 52:08-52:44, 53:01:01-53:39, 58:01:0158:02, 58:04-58:10N, 58:12-58:19, 58:21-58:29, 58:31N-58:69, 78:01:01-78:08
*27:01-27:11, 27:13-27:15, 27:17, 27:19-27:21, 27:25-27:28, 27:30-27:38, 27:40-27:58,
27:60-27:76, 27:78-27:91, 27:93-27:118, 27:120-27:128, 27:130-27:135, 44:90, 44:97,
54:01:01-54:01:06, 54:03-54:29, 54:31-54:34, 55:01:01-55:05, 55:07-55:17, 55:19-55:70,
56:01:01:01-56:04, 56:06-56:16, 56:18-56:20:02, 56:22, 56:24-56:32, 56:34-56:35, 56:3756:41, 56:43, 56:45, 82:01-82:03
*27:24
*35:66
*35:76, 35:186, 35:229, 56:05:01-56:05:02, 56:21, 56:36
*37:52, 38:11, C*03:137, C*03:182, C*07:351, C*12:82
*40:31, 40:45, 40:80, 48:01:01-48:01:06, 48:06-48:07, 48:09, 48:11, 48:15-48:16, 48:1848:20, 48:22, 48:27-48:35
*48:04, 48:24
*48:36
*54:02
*67:02
*67:05
*83:01
C*07:102
Well No.
Visit www.olerup-ssp.com
for “Instructions for Use”
(IFU) and detailed Product
Inserts
1
2
1
1
1
1
1
1
1
2
?
?
?
?
?
3
4
3
?
?
?
?
?
4
4
4
4
4
4
4
3
4
5
6
7
8
9
5
5
6
6
7
8
3
4
3
2
3
4
4
4
Negative Control
April 2015
Rev. No: 00
7
5
6
5
2
1
2
4
2
5
5
7
1
4
1
1
4
7
7
7
2
3
3
5
?
2
1
2
3
4
5
6
w
7
8
9
1HLA-B*81
alleles in bold lettering are listed as confirmed alleles on the on the IMGT/HLA web page
www.ebi.ac.uk/imgt/hla, release 3.19.0, January 2015.
2Alleles that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last
IMGT/HLA database release can be retrieved from web page http://hla.alleles.org/alleles/deleted.html.
3The B*81:02 and B*07:174, B*07:202 and B*07:2224 alleles will give rise to identical amplification patterns. These alleles
can e.g. be distinguished by the HLA-B low resolution kit and/or HLA-B*07 high resolution kit.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 2 of 3
®
April 2015
Rev. No: 00
HLA-B*81
101.553-06/06u
99X
Worksheet
Visit www.olerup-ssp.com
for “Instructions for Use”
(IFU) and detailed Product
Inserts
4Primer
mix 5: Specific PCR fragment of 105 bp in the B*81:05 and the B*07:83 alleles. Specific PCR fragment of 165
bp in the B*81:03 and the B*15:20, 15:228, 15:305, 35:01:01:01-35:01:27, 35:01:29-35:30, 35:32:01-35:34, 35:3635:42:02, 35:44-35:45, 35:47-35:56, 35:58-35:59, 35:61:01-35:72, 35:74-35:78, 35:80-35:86, 35:88-35:184,
35:186-35:278, 48:02:01-48:02:03, 51:01:01:01-51:24:05, 51:26-51:41N, 51:43-51:46, 51:48-51:156, 51:158:0151:163, 51:165-51:185, 52:01:01:01-52:06:02, 52:08-52:44, 53:01:01-53:39, 56:05:01-56:05:02, 56:21, 56:36,
58:01:01-58:02, 58:04-58:10N, 58:12-58:19, 58:21-58:29, 58:31N-58:69, 67:05, 78:01:01-78:08 and 83:01? alleles.
Primer mix 6: Specific PCR fragment of 80 bp in the B*81:04N allele. Specific PCR fragment of 105 bp in the B*81:05
and the B*07:83 alleles.
‘w’, may be weakly amplified.
‘?’, the nucleotide sequence of the primer matching region is not known.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 3 of 3