Standardized Syllabus for the College of Engineering
BME 3323L Cellular Engineering Laboratory
(Finalized syllabus will be distributed on first day of class)
1. Catalog Description
(3 credit hours)
The Cellular Engineering Laboratory will teach students the fundamentals of cell
culture for use in Biomedical Engineering investigations. Students will acquire skills
in cell culture, quantitative analyses, notebook keeping, report writing and oral
presentation.
2. Pre-requisites and Co-requisites
Pre-requisites:
BSC 2010, CHM 2046
Co-requisites:
BCH 4024
PCB 3XXX Cell & Systems Physiology
3. Course Objectives
1) To introduce the student to the concept of cell culture as a research tool.
2) To introduce the student to the concept of growing healthy and happy cells in an in
vitro environment, that is critical to conducting successful in vitro research.
3) To give student hands-on experience growing cells in an in vitro environment.
4) To introduce the student to the concept of designing, executing and analyzing and
summarizing data derived from an experiment.
4. Contribution of course to meeting the professional component (ABET only)
5. Relationship of course to program outcomes (ABET only)
6. Instructor - TBA
a. Office location
b. Telephone
c. E-mail address
d. Web site
e. Office hours
7. Teaching Assistant - TBA
a. Office location
b. Telephone
c. E-mail address
d. Office hours
8. Meeting Times
Course meets thrice per week, schedule TBA
9. Class/laboratory schedule
Class meets for:
a) 1 - 50 min lecture per week (Tues – Time TBA)
b) 1 – 230 min laboratory (Thursday – Time TBA)
c) Short time periods on Monday to tend to cells (arranged with TA)
10. Meeting Location – TBA
11. Material and Supply Fees – TBA
12. Textbooks and Software Required
Culture of Animal Cells
R. Ian Freshney
2005, 5th Edition
ISBN 0471453293
Laboratory Manual (course notes to be supplied by instructor):
Laboratory Manual for Cellular Engineering
Brandi K. Ormerod and Crystal L. Stephens
2009
Instructor prepared and distributed
Other Requirements:
Standard laboratory notebook with lined paper
Hair ties (elastic bands) to tie back long hair
Safety Glasses
13. Recommended Reading
(see 12 above)
14. Course Outline
Week 1 – Lecture (Tues-1hr): History and Introduction to Cell Culture
Laboratory (Thurs-4hr)
Week 2 – Lecture (Tues – 1hr): Types of Cell Culture
Laboratory (Thurs-4hr)
Week 3 – Lecture (Tues-1hr): Isolation of Cells for Culture
Laboratory (Thurs-4hr)
Week 4 – Lecture (Tues-1hr): Subculture
Laboratory (Thurs-4hr)
Week 5 – Lecture (Tues-1hr): Cryopreservation
Laboratory (Thurs-4hr)
Week 6 – Lecture (Tues-1hr): Contamination
Laboratory (Thurs-4hr)
Week 7 – Lecture (Tues-1hr): Record Keeping
Laboratory (Thurs-4hr)
Report #1 Due
Week 8 – Lecture (Tues-1hr): Review (Weeks 1-7)
Laboratory (Thurs-4hr): Exam#1 (Weeks 1-7)
Week 9 – Lecture (Tues-1hr): Preparation of Primary Cells
Laboratory (Thurs-4hrs)
Week 10 – Lecture (Tues-1hr): Characterization
Laboratory (Thurs-4hr)
Week 11 – Lecture (Tues-1hr): Differentiation
Laboratory (Thurs-4hr)
Week 12 – Lecture (Tues-1hr): Quantitation
Laboratory (Thurs-4hr)
Week 13 – Lecture (Tues-1hr): Specific Cell Lines
Laboratory (Thurs-4hr)
Week 14 – Lecture (Tues-1hr): Specific Cell Lines
Laboratory (Thurs-4hr)
Report #2 Due
Week 15 – Lecture (Tues-1hr): Review
Laboratory (Thurs-4hr): Exam# 2 (Weeks 8-14)
15. Attendance and Expectations
Class participation is required and part of the final grade Grading – methods of
evaluation (e.g., quizzes 20%, homework 15%, term paper 30%, final exam 35%)
16. Grading
Report #1 – 15%; Report #2 – 15%, Exam#1 – 25%, Exam#2 - 25%, Lab Note Book
– 20%
17. Grading Scale
A
AB+
B
B-
C+
C
C-
D+
D
D-
E
> 90
80-83
77-79
74-76
70-73
67-69
64-66
60-63
57-59
< 56
87-89
84-86
18. Make-up Exam Policy
Midterm and Final Exam can be made up in extreme circumstances if a Doctor’s note
is presented or death certificate (regarding someone in the immediate family) is
presented before the start of the exam.
19. Honesty Policy
All students admitted to the University of Florida have signed a statement of
academic honesty committing themselves to be honest in all academic work and
understanding that failure to comply with this commitment will result in disciplinary
action. This statement is a reminder to uphold your obligation as a UF student and to
be honest in all work submitted and exams taken in this course and all others.
20. Accommodation for Students with Disabilities
Students Requesting classroom accommodation must first register with the Dean of
Students Office. That office will provide the student with documentation that he/she
must provide to the course instructor when requesting accommodation.
21. UF Counseling Services
Resources are available on-campus for students having personal problems or lacking
clear career and academic goals. The resources include:
- University Counseling Center, 301 Peabody Hall, 392-1575, Personal and
Career Counseling.
- SHCC mental Health, Student Health Care Center, 392-1171, Personal and
Counseling.
- Center for Sexual Assault/Abuse Recovery and Education (CARE), Student
Health Care Center, 392-1161, sexual assault counseling.
- Career Resource Center, Reitz Union, 392-1601, career development assistance
and counseling.
22. Software Use
All faculty, staff and student of the University are required and expected to obey the
laws and legal agreements governing software use. Failure to do so can lead to
monetary damages and/or criminal penalties for the individual violator. Because such
violations are also against University policies and rules, disciplinary action will be
taken as appropriate. We, the members of the University of Florida community,
pledge to uphold ourselves and our peers to the highest standards of honesty and
integrity.